DOI: 10.1126/science.7716545. Adaptive Mutation in <i>Escherichia coli</i> : a Role for Conjugation

Adaptive Mutation in Escherichia coli : a Role for Conjugation

10.1126/science.7716545

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

When subjected to selective conditions that impose starvation, a bacterial population can accumulate mutations, called adaptive, that allow colony formation. Here, the reversion of a lac allele under selective conditions, in a model system using Escherichia coli with the lac mutation on an F' plasmid, was shown to require the conjugational capacity of the plasmid. Reversion associated with transfer was shown, and when the same lac allele was chromosomal, reversion to Lac + was 25 to 50 times less frequent. Postplating reversion was 25 times less when mating was inhibited by the presence of detergent. Mutability associated with conjugation provides new ways of thinking about the origin of adaptive mutations.

Bibliography

Radicella, J. P., Park, P. U., & Fox, M. S. (1995). Adaptive Mutation in Escherichia coli â¯: a Role for Conjugation. Science, 268(5209), 418â420.

Authors 3

J. Pablo Radicella (first)
Peter U. Park (additional)
Maurice S. Fox (additional)

References 34 Referenced 95

ACHTMAN, M, CELL-CELL INTERACTIONS IN CONJUGATING ESCHERICHIA-COLI - ROLE OF F-PILI AND FATE OF MATING AGGREGATES, JOURNAL OF BACTERIOLOGY 135: 1053 (1978). (10.1128/jb.135.3.1053-1061.1978) / JOURNAL OF BACTERIOLOGY (1978)
10.1093/genetics/128.4.695
10.1038/335142a0
CHARLESWORTH, D, ORIGIN OF MUTANTS DISPUTED, NATURE 336: 525 (1988). (10.1038/336525b0) / NATURE (1988)
FOSTER, P.L., ADAPTIVE MUTATION - THE USES OF ADVERSITY, ANNUAL REVIEW OF MICROBIOLOGY 47: 467 (1993). (10.1146/annurev.mi.47.100193.002343) / ANNUAL REVIEW OF MICROBIOLOGY (1993)
10.1093/genetics/131.4.783
FOSTER, P.L., POPULATION-DYNAMICS OF A LAC(-) STRAIN OF ESCHERICHIA-COLI DURING SELECTION FOR LACTOSE UTILIZATION, GENETICS 138: 253 (1994). (10.1093/genetics/138.2.253) / GENETICS (1994)
Foster, P. L., Proceedings of the National Academy of Sciences of the United States of America 92: 5487 (1995). / Proceedings of the National Academy of Sciences of the United States of America (1995)
10.1126/science.8023164
FOX, M, MOLECULAR & GENERAL GENETICS 240: 355 (1993). (10.1007/BF00280386) / MOLECULAR & GENERAL GENETICS (1993)
10.1126/science.7716546
10.1126/science.8146657
HAYES, W, RECOMBINATION IN BACT-COLI K-12 - UNIDIRECTIONAL TRANSFER OF GENETIC MATERIAL, NATURE 169: 118 (1952). / NATURE (1952)
LEDERBERG, J, SEX COMPATIBILITY IN ESCHERICHIA-COLI, GENETICS 37: 720 (1952). (10.1093/genetics/37.6.720) / GENETICS (1952)
LEDERBERG, J, REPLICA PLATING AND INDIRECT SELECTION OF BACTERIAL MUTANTS, JOURNAL OF BACTERIOLOGY 63: 399 (1952). (10.1128/jb.63.3.399-406.1952) / JOURNAL OF BACTERIOLOGY (1952)
LENSKI, R.E., NATURE 336: 123 (1988). (10.1038/336123a0) / NATURE (1988)
10.1126/science.7678468
10.1126/science.260.5112.1222
LICHTEN, M, EFFECTS OF NONHOMOLOGY ON BACTERIOPHAGE-LAMBDA RECOMBINATION, GENETICS 103: 5 (1983). (10.1093/genetics/103.1.5) / GENETICS (1983)
10.1093/genetics/28.6.491
Miller, J. H., Experiments in Molecular Genetics: 431 (1972). / Experiments in Molecular Genetics (1972)
NOVICK, A, EXPERIMENTS WITH THE CHEMOSTAT ON SPONTANEOUS MUTATIONS OF BACTERIA, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 36: 708 (1950). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1950)
PARTRIDGE, L, IS BACTERIAL EVOLUTION RANDOM OR SELECTIVE, NATURE 336: 22 (1988). (10.1038/336022a0) / NATURE (1988)
PETERS, J.E., REDUNDANT TRANSFER OF F' PLASMIDS OCCURS BETWEEN ESCHERICHIA-COLI-CELLS DURING NONLETHAL SELECTIONS, JOURNAL OF BACTERIOLOGY 177: 847 (1995). (10.1128/jb.177.3.847-850.1995) / JOURNAL OF BACTERIOLOGY (1995)
10.1126/science.8023163
RYAN, F.J., BACTERIAL MUTATION IN A STATIONARY PHASE AND THE QUESTION OF CELL TURNOVER, JOURNAL OF GENERAL MICROBIOLOGY 21: 530 (1959). (10.1099/00221287-21-3-530) / JOURNAL OF GENERAL MICROBIOLOGY (1959)
RYAN, F.J., IS DNA REPLICATION A NECESSARY CONDITION FOR SPONTANEOUS MUTATION, ZEITSCHRIFT FUR VERERBUNGSLEHRE 92: 38 (1961). / ZEITSCHRIFT FUR VERERBUNGSLEHRE (1961)
SHAPIRO, J. A., MOLECULAR & GENERAL GENETICS 194: 79 (1984). (10.1007/BF00383501) / MOLECULAR & GENERAL GENETICS (1984)
SINGER, M, A COLLECTION OF STRAINS CONTAINING GENETICALLY LINKED ALTERNATING ANTIBIOTIC-RESISTANCE ELEMENTS FOR GENETIC-MAPPING OF ESCHERICHIA-COLI, MICROBIOLOGICAL REVIEWS 53: 1 (1989). (10.1128/mr.53.1.1-24.1989) / MICROBIOLOGICAL REVIEWS (1989)
STAHL, F.W., BACTERIAL GENETICS - A UNICORN IN THE GARDEN, NATURE 335: 112 (1988). / NATURE (1988)
STAHL, F.W., UNICORNS REVISITED, GENETICS 132: 865 (1992). (10.1093/genetics/132.4.865) / GENETICS (1992)
STEWART, F.M., FLUCTUATION ANALYSIS - THE PROBABILITY-DISTRIBUTION OF THE NUMBER OF MUTANTS UNDER DIFFERENT CONDITIONS, GENETICS 124: 175 (1990). (10.1093/genetics/124.1.175) / GENETICS (1990)
SYMONDS, N, EVOLUTION - ANTICIPATORY MUTAGENESIS, NATURE 337: 119 (1989). (10.1038/337119a0) / NATURE (1989)
Tessman, I., Nature 336:527 (1988). (10.1038/336527b0) / Nature (1988)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 2:19 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:41 p.m.)
Indexed	4 weeks, 2 days ago (Aug. 7, 2025, 4:29 p.m.)
Issued	30 years, 4 months ago (April 21, 1995)
Published	30 years, 4 months ago (April 21, 1995)
Published Print	30 years, 4 months ago (April 21, 1995)

Funders 0

None

BibTeX

@article{Radicella_1995, title={Adaptive Mutation in Escherichia coli  : a Role for Conjugation}, volume={268}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7716545}, DOI={10.1126/science.7716545}, number={5209}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Radicella, J. Pablo and Park, Peter U. and Fox, Maurice S.}, year={1995}, month=apr, pages={418–420} }

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