DOI: 10.1126/science.7682336. Spatially Resolved Dynamics of cAMP and Protein Kinase A Subunits in <i>Aplysia</i> Sensory Neurons

Spatially Resolved Dynamics of cAMP and Protein Kinase A Subunits in Aplysia Sensory Neurons

10.1126/science.7682336

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Cyclic adenosine monophosphate (cAMP)-dependent protein kinase, labeled with fluorescein and rhodamine on the catalytic and regulatory subunits, respectively, was injected into Aplysia sensory neurons either in culture or in intact cell clusters. Energy transfer between the subunits, a measure of cytosolic cAMP concentration ([cAMP]), and compartmentation of the dissociated subunits were monitored by confocal fluorescence microscopy. Bath application of serotonin produced a much greater elevation of [cAMP] in the processes than in the central bodies of the neurons. The resulting gradients must drive a sizable centripetal flux of cAMP because direct microinjection of cAMP showed that it diffused readily. Perinuclear increases in [cAMP] slowly caused the translocation of the freed catalytic subunit into the nucleus to an extent proportional to the percentage of its dissociation from the regulatory subunit.

Bibliography

Bacskai, B. J., Hochner, B., Mahaut-Smith, M., Adams, S. R., Kaang, B.-K., Kandel, E. R., & Tsien, R. Y. (1993). Spatially Resolved Dynamics of cAMP and Protein Kinase A Subunits in Aplysia Sensory Neurons. Science, 260(5105), 222â226.

Authors 7

Brian J. Bacskai (first)
Binyamin Hochner (additional)
Martyn Mahaut-Smith (additional)
Stephen R. Adams (additional)
Bong-Kiun Kaang (additional)
Eric R. Kandel (additional)
Roger Y. Tsien (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 7:05 p.m.)
Deposited	1 year, 7 months ago (Jan. 15, 2024, 12:28 a.m.)
Indexed	1 month, 4 weeks ago (July 1, 2025, 6:30 a.m.)
Issued	32 years, 4 months ago (April 9, 1993)
Published	32 years, 4 months ago (April 9, 1993)
Published Print	32 years, 4 months ago (April 9, 1993)

Funders 0

None

BibTeX

@article{Bacskai_1993, title={Spatially Resolved Dynamics of cAMP and Protein Kinase A Subunits in Aplysia Sensory Neurons}, volume={260}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7682336}, DOI={10.1126/science.7682336}, number={5105}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Bacskai, Brian J. and Hochner, Binyamin and Mahaut-Smith, Martyn and Adams, Stephen R. and Kaang, Bong-Kiun and Kandel, Eric R. and Tsien, Roger Y.}, year={1993}, month=apr, pages={222–226} }

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