DOI: 10.1126/science.7624799. Cloning and Characterization of a G Protein-Activated Human Phosphoinositide-3 Kinase

Cloning and Characterization of a G Protein-Activated Human Phosphoinositide-3 Kinase

10.1126/science.7624799

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Phosphoinositide-3 kinase activity is implicated in diverse cellular responses triggered by mammalian cell surface receptors and in the regulation of protein sorting in yeast. Receptors with intrinsic and associated tyrosine kinase activity recruit heterodimeric phosphoinositide-3 kinases that consist of p110 catalytic subunits and p85 adaptor molecules containing Src homology 2 (SH2) domains. A phosphoinositide-3 kinase isotype, p110γ, was cloned and characterized. The p110γ enzyme was activated in vitro by both the α and βγ subunits of heterotrimeric guanosine triphosphate (GTP)-binding proteins (G proteins) and did not interact with p85. A potential pleckstrin homology domain is located near its amino terminus. The p110γ isotype may link signaling through G protein-coupled receptors to the generation of phosphoinositide second messengers phosphorylated in the D-3 position.

Bibliography

Stoyanov, B., Volinia, S., Hanck, T., Rubio, I., Loubtchenkov, M., Malek, D., Stoyanova, S., Vanhaesebroeck, B., Dhand, R., NÃ¼rnberg, B., Gierschik, P., Seedorf, K., Hsuan, J. J., Waterfield, M. D., & Wetzker, R. (1995). Cloning and Characterization of a G Protein-Activated Human Phosphoinositide-3 Kinase. Science, 269(5224), 690â693.

Authors 15

Borislav Stoyanov (first)
Stefano Volinia (additional)
Theodor Hanck (additional)
Ignacio Rubio (additional)
Michael Loubtchenkov (additional)
Daria Malek (additional)
Stefka Stoyanova (additional)
Bart Vanhaesebroeck (additional)
Ritu Dhand (additional)
Bernd Nürnberg (additional)
Peter Gierschik (additional)
Klaus Seedorf (additional)
J. Justin Hsuan (additional)
Michael D. Waterfield (additional)
Reinhard Wetzker (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 2:19 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:51 p.m.)
Indexed	1 month ago (July 26, 2025, 4:41 a.m.)
Issued	30 years ago (Aug. 4, 1995)
Published	30 years ago (Aug. 4, 1995)
Published Print	30 years ago (Aug. 4, 1995)

Funders 0

None

BibTeX

@article{Stoyanov_1995, title={Cloning and Characterization of a G Protein-Activated Human Phosphoinositide-3 Kinase}, volume={269}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7624799}, DOI={10.1126/science.7624799}, number={5224}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Stoyanov, Borislav and Volinia, Stefano and Hanck, Theodor and Rubio, Ignacio and Loubtchenkov, Michael and Malek, Daria and Stoyanova, Stefka and Vanhaesebroeck, Bart and Dhand, Ritu and Nürnberg, Bernd and Gierschik, Peter and Seedorf, Klaus and Hsuan, J. Justin and Waterfield, Michael D. and Wetzker, Reinhard}, year={1995}, month=aug, pages={690–693} }

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