DOI: 10.1126/science.7624781. Activation of Yeast PBS2 MAPKK by MAPKKKs or by Binding of an SH3-Containing Osmosensor

Activation of Yeast PBS2 MAPKK by MAPKKKs or by Binding of an SH3-Containing Osmosensor

10.1126/science.7624781

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The role of mitogen-activated protein (MAP) kinase cascades in integrating distinct upstream signals was studied in yeast. Mutants that were not able to activate PBS2 MAP kinase kinase (MAPKK; Pbs2p) at high osmolarity were characterized. Pbs2p was activated by two independent signals that emanated from distinct cell-surface osmosensors. Pbs2p was activated by MAP kinase kinase kinases (MAPKKKs) Ssk2p and Ssk22p that are under the control of the SLN1-SSK1 two-component osmosensor. Alternatively, Pbs2p was activated by a mechanism that involves the binding of its amino terminal proline-rich motif to the Src homology 3 (SH3) domain of a putative transmembrane osmosensor Sho1p.

Bibliography

Maeda, T., Takekawa, M., & Saito, H. (1995). Activation of Yeast PBS2 MAPKK by MAPKKKs or by Binding of an SH3-Containing Osmosensor. Science, 269(5223), 554â558.

Authors 3

Tatsuya Maeda (first)
Mutsuhiro Takekawa (additional)
Haruo Saito (additional)

References 23 Referenced 513

ALESSI, D.R., IDENTIFICATION OF THE SITES IN MAP KINASE KINASE-1 PHOSPHORYLATED BY P74(RAF-1), EMBO JOURNAL 13: 1610 (1994). (10.1002/j.1460-2075.1994.tb06424.x) / EMBO JOURNAL (1994)
BOGUSLAWSKI, G, PBS2, A YEAST GENE ENCODING A PUTATIVE PROTEIN-KINASE, INTERACTS WITH THE RAS2 PATHWAY AND AFFECTS OSMOTIC SENSITIVITY OF SACCHAROMYCES-CEREVISIAE, JOURNAL OF GENERAL MICROBIOLOGY 138: 2425 (1992). (10.1099/00221287-138-11-2425) / JOURNAL OF GENERAL MICROBIOLOGY (1992)
10.1126/science.7681220
CAIRNS, B.R., ORDER OF ACTION OF COMPONENTS IN THE YEAST PHEROMONE RESPONSE PATHWAY REVEALED WITH A DOMINANT ALLELE OF THE STE11-KINASE AND THE MULTIPLE PHOSPHORYLATION OF THE STE7-KINASE, GENES & DEVELOPMENT 6: 1305 (1992). (10.1101/gad.6.7.1305) / GENES & DEVELOPMENT (1992)
CHIEN, C.T., THE 2-HYBRID SYSTEM - A METHOD TO IDENTIFY AND CLONE GENES FOR PROTEINS THAT INTERACT WITH A PROTEIN OF INTEREST, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 88: 9578 (1991). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1991)
COHEN, G.B., MODULAR BINDING DOMAINS IN SIGNAL-TRANSDUCTION PROTEINS, CELL 80: 237 (1995). (10.1016/0092-8674(95)90406-9) / CELL (1995)
COOPER, J.A., MAP KINASE PATHWAYS - STRAIGHT AND NARROW OR TORTUOUS AND INTERSECTING, CURRENT BIOLOGY 4: 1118 (1994). (10.1016/S0960-9822(00)00251-7) / CURRENT BIOLOGY (1994)
DAVIS, R.J., MAPKS - NEW JNK EXPANDS THE GROUP, TRENDS IN BIOCHEMICAL SCIENCES 19: 470 (1994). (10.1016/0968-0004(94)90132-5) / TRENDS IN BIOCHEMICAL SCIENCES (1994)
10.1126/science.7839144
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10.1126/science.7914033
10.1016/0092-8674(95)90402-6
IRIE, K, MKK1 AND MKK2, WHICH ENCODE SACCHAROMYCES-CEREVISIAE MITOGEN-ACTIVATED PROTEIN KINASE-KINASE HOMOLOGS, FUNCTION IN THE PATHWAY MEDIATED BY PROTEIN-KINASE-C, MOLECULAR AND CELLULAR BIOLOGY 13: 3076 (1993). / MOLECULAR AND CELLULAR BIOLOGY (1993)
10.1016/0022-2836(82)90515-0
10.1038/369242a0
MAEDA T unpublished data.
10.1038/357038a0
10.1126/science.8211183
10.1038/373573a0
ROTHSTEIN, R, TARGETING, DISRUPTION, REPLACEMENT, AND ALLELE RESCUE - INTEGRATIVE DNA TRANSFORMATION IN YEAST, METHODS IN ENZYMOLOGY 194: 281 (1991). (10.1016/0076-6879(91)94022-5) / METHODS IN ENZYMOLOGY (1991)
10.1016/0092-8674(94)90277-1
10.1038/372794a0
10.1016/0092-8674(93)90307-C

Dates

Type	When
Created	18 years, 9 months ago (Oct. 27, 2006, 2:19 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:48 p.m.)
Indexed	4 days, 9 hours ago (Aug. 19, 2025, 6:11 a.m.)
Issued	30 years ago (July 28, 1995)
Published	30 years ago (July 28, 1995)
Published Print	30 years ago (July 28, 1995)

Funders 0

None

BibTeX

@article{Maeda_1995, title={Activation of Yeast PBS2 MAPKK by MAPKKKs or by Binding of an SH3-Containing Osmosensor}, volume={269}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7624781}, DOI={10.1126/science.7624781}, number={5223}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Maeda, Tatsuya and Takekawa, Mutsuhiro and Saito, Haruo}, year={1995}, month=jul, pages={554–558} }

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