DOI: 10.1126/science.7618104. Telomerase in Yeast

Telomerase in Yeast

10.1126/science.7618104

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The ribonucleoprotein enzyme telomerase synthesizes telomeric DNA by copying an internal RNA template sequence. The telomerase activities of the yeasts Saccharomyces castellii and Saccharomyces cerevisiae —with regular and irregular telomeric sequences, respectively—have now been identified and characterized. The S. cerevisiae activity required the telomerase RNA gene TLC1 but not the EST1 gene, both of which are required for normal telomere maintenance in vivo. This activity exhibited low processivity and produced no regularly repeated products. An inherently high stalling frequency of the S. cerevisiae telomerase may account for its in vitro properties and for the irregular telomeric sequences of this yeast.

Bibliography

Cohn, M., & Blackburn, E. H. (1995). Telomerase in Yeast. Science, 269(5222), 396â400.

Authors 2

Marita Cohn (first)
Elizabeth H. Blackburn (additional)

References 30 Referenced 229

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BLACKBURN, E.H., THE MOLECULAR-STRUCTURE OF CENTROMERES AND TELOMERES, ANNUAL REVIEW OF BIOCHEMISTRY 53: 163 (1984). (10.1146/annurev.bi.53.070184.001115) / ANNUAL REVIEW OF BIOCHEMISTRY (1984)
BLACKBURN, E.H., TELOMERES - NO END IN SIGHT, CELL 77: 621 (1994). (10.1016/0092-8674(94)90046-9) / CELL (1994)
COHN M unpublished data.
COLLINS, K, TETRAHYMENA TELOMERASE CATALYZES NUCLEOLYTIC CLEAVAGE AND NONPROCESSIVE ELONGATION, GENES & DEVELOPMENT 7: 1364 (1993). (10.1101/gad.7.7b.1364) / GENES & DEVELOPMENT (1993)
Gilley, D., Genes & Development 9:2214 (1995). (10.1101/gad.9.18.2214) / Genes & Development (1995)
GREIDER, C.W., IDENTIFICATION OF A SPECIFIC TELOMERE TERMINAL TRANSFERASE-ACTIVITY IN TETRAHYMENA EXTRACTS, CELL 43: 405 (1985). (10.1016/0092-8674(85)90170-9) / CELL (1985)
GREIDER, C.W., THE TELOMERE TERMINAL TRANSFERASE OF TETRAHYMENA IS A RIBONUCLEOPROTEIN ENZYME WITH 2 KINDS OF PRIMER SPECIFICITY, CELL 51: 887 (1987). (10.1016/0092-8674(87)90576-9) / CELL (1987)
GREIDER, C.W., A TELOMERIC SEQUENCE IN THE RNA OF TETRAHYMENA TELOMERASE REQUIRED FOR TELOMERE REPEAT SYNTHESIS, NATURE 337: 331 (1989). (10.1038/337331a0) / NATURE (1989)
HARRINGTON, L.A., TELOMERASE PRIMER SPECIFICITY AND CHROMOSOME HEALING, NATURE 353: 451 (1991). (10.1038/353451a0) / NATURE (1991)
LEE, M.S., SEQUENCE-SPECIFIC DNA PRIMER EFFECTS ON TELOMERASE POLYMERIZATION ACTIVITY, MOLECULAR AND CELLULAR BIOLOGY 13: 6586 (1993). / MOLECULAR AND CELLULAR BIOLOGY (1993)
LIN, J.J., AN IN-VITRO ASSAY FOR SACCHAROMYCES TELOMERASE REQUIRES EST1, CELL 81: 1127 (1995). (10.1016/S0092-8674(05)80017-0) / CELL (1995)
LINGNER, J, TELOMERASE RNAS OF DIFFERENT CILIATES HAVE A COMMON SECONDARY STRUCTURE AND A PERMUTED TEMPLATE, GENES & DEVELOPMENT 8: 1984 (1994). (10.1101/gad.8.16.1984) / GENES & DEVELOPMENT (1994)
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LUNDBLAD, V, RNA-DEPENDENT POLYMERASE MOTIFS IN EST1 - TENTATIVE IDENTIFICATION OF A PROTEIN-COMPONENT OF AN ESSENTIAL YEAST TELOMERASE, CELL 60: 529 (1990). (10.1016/0092-8674(90)90653-V) / CELL (1990)
LUNDBLAD, V, AN ALTERNATIVE PATHWAY FOR YEAST TELOMERE MAINTENANCE RESCUES EST1- SENESCENCE, CELL 73: 347 (1993). (10.1016/0092-8674(93)90234-H) / CELL (1993)
MANTELL, L.L., TELOMERASE ACTIVITY IN GERMLINE AND EMBRYONIC-CELLS OF XENOPUS, EMBO JOURNAL 13: 3211 (1994). (10.1002/j.1460-2075.1994.tb06620.x) / EMBO JOURNAL (1994)
McEachern , M. J., Nature 376: 403 (1995). (10.1038/376403a0) / Nature (1995)
MORIN, G.B., THE HUMAN TELOMERE TERMINAL TRANSFERASE ENZYME IS A RIBONUCLEOPROTEIN THAT SYNTHESIZES TTAGGG REPEATS, CELL 59: 521 (1989). (10.1016/0092-8674(89)90035-4) / CELL (1989)
PROWSE, K.R., IDENTIFICATION OF A NONPROCESSIVE TELOMERASE ACTIVITY FROM MOUSE CELLS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 90: 1493 (1993). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1993)
SHAMPAY, J, DNA-SEQUENCES OF TELOMERES MAINTAINED IN YEAST, NATURE 310: 154 (1984). (10.1038/310154a0) / NATURE (1984)
SHIPPENLENTZ, D, TELOMERE TERMINAL TRANSFERASE-ACTIVITY FROM EUPLOTES-CRASSUS ADDS LARGE NUMBERS OF TTTTGGGG REPEATS ONTO TELOMERIC PRIMERS, MOLECULAR AND CELLULAR BIOLOGY 9: 2761 (1989). / MOLECULAR AND CELLULAR BIOLOGY (1989)
10.1126/science.1689074
10.1126/science.7545955
WALMSLEY, R.M., UNUSUAL DNA-SEQUENCES ASSOCIATED WITH THE ENDS OF YEAST CHROMOSOMES, NATURE 310: 157 (1984). (10.1038/310157a0) / NATURE (1984)
WANG, S.S., SEQUENCING OF SACCHAROMYCES TELOMERES CLONED USING T4-DNA POLYMERASE REVEALS 2 DOMAINS, MOLECULAR AND CELLULAR BIOLOGY 10: 4415 (1990). / MOLECULAR AND CELLULAR BIOLOGY (1990)
WANG, S.S., TELOMERE TELOMERE RECOMBINATION PROVIDES AN EXPRESS PATHWAY FOR TELOMERE ACQUISITION, NATURE 345: 456 (1990). (10.1038/345456a0) / NATURE (1990)
YU, G.L., DEVELOPMENTALLY PROGRAMMED HEALING OF CHROMOSOMES BY TELOMERASE IN TETRAHYMENA, CELL 67: 823 (1991). (10.1016/0092-8674(91)90077-C) / CELL (1991)
YU, G.L., INVIVO ALTERATION OF TELOMERE SEQUENCES AND SENESCENCE CAUSED BY MUTATED TETRAHYMENA-TELOMERASE RNAS, NATURE 344: 126 (1990). (10.1038/344126a0) / NATURE (1990)
ZAHLER, A.M., TELOMERE TERMINAL TRANSFERASE-ACTIVITY IN THE HYPOTRICHOUS CILIATE OXYTRICHA-NOVA AND A MODEL FOR REPLICATION OF THE ENDS OF LINEAR DNA-MOLECULES, NUCLEIC ACIDS RESEARCH 16: 6953 (1988). (10.1093/nar/16.14.6953) / NUCLEIC ACIDS RESEARCH (1988)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 2:19 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:56 p.m.)
Indexed	2 months, 1 week ago (June 20, 2025, 12:05 a.m.)
Issued	30 years, 1 month ago (July 21, 1995)
Published	30 years, 1 month ago (July 21, 1995)
Published Print	30 years, 1 month ago (July 21, 1995)

Funders 0

None

BibTeX

@article{Cohn_1995, title={Telomerase in Yeast}, volume={269}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7618104}, DOI={10.1126/science.7618104}, number={5222}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Cohn, Marita and Blackburn, Elizabeth H.}, year={1995}, month=jul, pages={396–400} }

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    "issue": "5222",
    "published-print": {
      "date-parts": [
        [
          1995,
          7,
          21
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.7618104"
  ],
  "URL": "http://dx.doi.org/10.1126/science.7618104",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1995,
        7,
        21
      ]
    ]
  }
}