DOI: 10.1126/science.7604281. Conversion of Bacteriorhodopsin into a Chloride Ion Pump

Conversion of Bacteriorhodopsin into a Chloride Ion Pump

10.1126/science.7604281

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

In the light-driven proton pump bacteriorhodopsin, proton transfer from the retinal Schiff base to aspartate-85 is the crucial reaction of the transport cycle. In halorhodopsin, a light-driven chloride ion pump, the equivalent of residue 85 is threonine. When aspartate-85 was replaced with threonine, the mutated bacteriorhodopsin became a chloride ion pump when expressed in Halobacterium salinarium and, like halorhodopsin, actively transported chloride ions in the direction opposite from the proton pump. Chloride was bound to it, as revealed by large shifts of the absorption maximum of the chromophore, and its photointermediates included a red-shifted state in the millisecond time domain, with its amplitude and decay rate dependent on chloride concentration. Bacteriorhodopsin and halorhodopsin thus share a common transport mechanism, and the interaction of residue 85 with the retinal Schiff base determines the ionic specificity.

Bibliography

Sasaki, J., Brown, L. S., Chon, Y.-S., Kandori, H., Maeda, A., Needleman, R., & Lanyi, J. K. (1995). Conversion of Bacteriorhodopsin into a Chloride Ion Pump. Science, 269(5220), 73â75.

Authors 7

Jun Sasaki (first)
Leonid S. Brown (additional)
Young-Shin Chon (additional)
Hideki Kandori (additional)
Akio Maeda (additional)
Richard Needleman (additional)
Janos K. Lanyi (additional)

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Dates

Type	When
Created	18 years, 9 months ago (Oct. 27, 2006, 2:19 p.m.)
Deposited	1 year, 7 months ago (Jan. 15, 2024, 12:24 a.m.)
Indexed	1 month ago (July 26, 2025, 5:34 a.m.)
Issued	30 years, 1 month ago (July 7, 1995)
Published	30 years, 1 month ago (July 7, 1995)
Published Print	30 years, 1 month ago (July 7, 1995)

Funders 0

None

BibTeX

@article{Sasaki_1995, title={Conversion of Bacteriorhodopsin into a Chloride Ion Pump}, volume={269}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7604281}, DOI={10.1126/science.7604281}, number={5220}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Sasaki, Jun and Brown, Leonid S. and Chon, Young-Shin and Kandori, Hideki and Maeda, Akio and Needleman, Richard and Lanyi, Janos K.}, year={1995}, month=jul, pages={73–75} }

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