DOI: 10.1126/science.7569928. Interaction of Tyrosine-Based Sorting Signals with Clathrin-Associated Proteins

Interaction of Tyrosine-Based Sorting Signals with Clathrin-Associated Proteins

10.1126/science.7569928

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Tyrosine-based signals within the cytoplasmic domain of integral membrane proteins mediate clathrin-dependent protein sorting in the endocytic and secretory pathways. A yeast two-hybrid system was used to identify proteins that bind to tyrosine-based signals. The medium chains (μ 1 and μ 2 ) of two clathrin-associated protein complexes (AP-1 and AP-2, respectively) specifically interacted with tyrosine-based signals of several integral membrane proteins. The interaction was confirmed by in vitro binding assays. Thus, it is likely that the medium chains serve as signal-binding components of the clathrin-dependent sorting machinery.

Bibliography

Ohno, H., Stewart, J., Fournier, M.-C., Bosshart, H., Rhee, I., Miyatake, S., Saito, T., Gallusser, A., Kirchhausen, T., & Bonifacino, J. S. (1995). Interaction of Tyrosine-Based Sorting Signals with Clathrin-Associated Proteins. Science, 269(5232), 1872â1875.

Authors 10

Hiroshi Ohno (first)
Jay Stewart (additional)
Marie-Christine Fournier (additional)
Herbert Bosshart (additional)
Ina Rhee (additional)
Shoichiro Miyatake (additional)
Takashi Saito (additional)
Andreas Gallusser (additional)
Tomas Kirchhausen (additional)
Juan S. Bonifacino (additional)

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Dates

Type	When
Created	18 years, 9 months ago (Oct. 27, 2006, 2:19 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:44 p.m.)
Indexed	2 days, 23 hours ago (Aug. 23, 2025, 1:02 a.m.)
Issued	29 years, 10 months ago (Sept. 29, 1995)
Published	29 years, 10 months ago (Sept. 29, 1995)
Published Print	29 years, 10 months ago (Sept. 29, 1995)

Funders 0

None

BibTeX

@article{Ohno_1995, title={Interaction of Tyrosine-Based Sorting Signals with Clathrin-Associated Proteins}, volume={269}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7569928}, DOI={10.1126/science.7569928}, number={5232}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Ohno, Hiroshi and Stewart, Jay and Fournier, Marie-Christine and Bosshart, Herbert and Rhee, Ina and Miyatake, Shoichiro and Saito, Takashi and Gallusser, Andreas and Kirchhausen, Tomas and Bonifacino, Juan S.}, year={1995}, month=sep, pages={1872–1875} }

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