DOI: 10.1126/science.7530860. Aminoacyl-RNA Synthesis Catalyzed by an RNA

Aminoacyl-RNA Synthesis Catalyzed by an RNA

10.1126/science.7530860

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

An RNA has been selected that rapidly aminoacylates its 2′(3′) terminus when provided with phenylalanyl-adenosine monophosphate. That is, the RNA accelerates the same aminoacyl group transfer catalyzed by protein aminoacyl-transfer RNA synthetases. The best characterized RNA reaction requires both Mg 2+ and Ca 2+ . These results confirm a necessary prediction of the RNA world hypothesis and represent efficient RNA reaction (≥ 10 5 times accelerated) at a carbonyl carbon, exemplifying a little explored type of RNA catalysis.

Bibliography

Illangasekare, M., Sanchez, G., Nickles, T., & Yarus, M. (1995). Aminoacyl-RNA Synthesis Catalyzed by an RNA. Science, 267(5198), 643â647.

Authors 4

Mali Illangasekare (first)
Giselle Sanchez (additional)
Tim Nickles (additional)
Michael Yarus (additional)

References 28 Referenced 276

10.1038/308820a0
10.1126/science.8128224
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BROWN, E.A., CALCIUM-BINDING TO NUCLEOTIDES - STRUCTURE OF A HYDRATED CALCIUM SALT OF INOSINE 5'-MONOPHOSPHATE, ACTA CRYSTALLOGRAPHICA SECTION B-STRUCTURAL SCIENCE 36: 2597 (1980). (10.1107/S0567740880009508) / ACTA CRYSTALLOGRAPHICA SECTION B-STRUCTURAL SCIENCE (1980)
CAVARELLI, J, THE ACTIVE-SITE OF YEAST ASPARTYL-TRANSFER-RNA SYNTHETASE - STRUCTURAL AND FUNCTIONAL-ASPECTS OF THE AMINOACYLATION REACTION, EMBO JOURNAL 13: 327 (1994). (10.1002/j.1460-2075.1994.tb06265.x) / EMBO JOURNAL (1994)
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10.1126/science.7513905
FERSHT, A.R., DISSECTION OF THE STRUCTURE AND ACTIVITY OF THE TYROSYL-TRANSFER RNA-SYNTHETASE BY SITE-DIRECTED MUTAGENESIS, BIOCHEMISTRY 26: 8031 (1987). (10.1021/bi00399a001) / BIOCHEMISTRY (1987)
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MCLAUGHLIN, C.S., CHEMICAL STUDIES ON AMINO ACID ACCEPTOR RIBONUCLEIC ACIDS .4. POSITION OF AMINO ACID RESIDUE IN AMINOACYL S-RNA - CHEMICAL APPROACH, BIOCHEMISTRY 4: 1442 (1965). (10.1021/bi00883a032) / BIOCHEMISTRY (1965)
MILLIGAN, J.F., OLIGORIBONUCLEOTIDE SYNTHESIS USING T7 RNA-POLYMERASE AND SYNTHETIC DNA TEMPLATES, NUCLEIC ACIDS RESEARCH 15: 8783 (1987). (10.1093/nar/15.21.8783) / NUCLEIC ACIDS RESEARCH (1987)
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10.1126/science.3381099

Dates

Type	When
Created	18 years, 9 months ago (Oct. 27, 2006, 2:19 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 10:58 p.m.)
Indexed	1 month, 2 weeks ago (July 7, 2025, 5:26 a.m.)
Issued	30 years, 6 months ago (Feb. 3, 1995)
Published	30 years, 6 months ago (Feb. 3, 1995)
Published Print	30 years, 6 months ago (Feb. 3, 1995)

Funders 0

None

BibTeX

@article{Illangasekare_1995, title={Aminoacyl-RNA Synthesis Catalyzed by an RNA}, volume={267}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7530860}, DOI={10.1126/science.7530860}, number={5198}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Illangasekare, Mali and Sanchez, Giselle and Nickles, Tim and Yarus, Michael}, year={1995}, month=feb, pages={643–647} }

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