DOI: 10.1126/science.7518616. Specific Interaction of Type I Receptors of the TGF-β Family with the Immunophilin FKBP-12

Specific Interaction of Type I Receptors of the TGF-β Family with the Immunophilin FKBP-12

10.1126/science.7518616

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Transforming growth factor-β (TGF-β) family members bind to receptors that consist of heteromeric serine-threonine kinase subunits (type I and type II). In a yeast genetic screen, the immunophilin FKBP-12, a target of the macrolides FK506 and rapamycin, interacted with the type I receptor for TGF-β and with other type I receptors. Deletion, point mutation, and co-immunoprecipitation studies further demonstrated the specificity of the interaction. Excess FK506 competed with type I receptors for binding to FKBP-12, which suggests that these receptors share or overlap the macrolide binding site on FKBP-12, and therefore they may represent its natural ligand. The specific interaction between the type I receptors and FKBP-12 suggests that FKBP-12 may play a role in type I receptor-mediated signaling.

Bibliography

Wang, T., Donahoe, P. K., & Zervos, A. S. (1994). Specific Interaction of Type I Receptors of the TGF-Î² Family with the Immunophilin FKBP-12. Science, 265(5172), 674â676.

Authors 3

Tongwen Wang (first)
Patricia K. Donahoe (additional)
Antonis S. Zervos (additional)

References 21 Referenced 265

ATTISANO, L, IDENTIFICATION OF HUMAN ACTIVIN AND TGF-BETA TYPE-I RECEPTORS THAT FORM HETEROMERIC KINASE COMPLEXES WITH TYPE-II RECEPTORS, CELL 75: 671 (1993). (10.1016/0092-8674(93)90488-C) / CELL (1993)
10.1126/science.8272871
10.1126/science.8388126
10.1126/science.8235612
10.1038/340245a0
FRAZEN, P, CELL 75: 681 (1993). (10.1016/0092-8674(93)90489-D) / CELL (1993)
GEORGI, L.L., DAF-1, A C-ELEGANS GENE CONTROLLING DAUER LARVA DEVELOPMENT, ENCODES A NOVEL RECEPTOR PROTEIN-KINASE, CELL 61: 635 (1990). (10.1016/0092-8674(90)90475-T) / CELL (1990)
10.1016/0092-8674(93)90498-F
HE, W.W., DEVELOPMENTAL EXPRESSION OF 4 NOVEL SERINE THREONINE KINASE RECEPTORS HOMOLOGOUS TO THE ACTIVIN TRANSFORMING GROWTH-FACTOR-BETA TYPE-II RECEPTOR FAMILY, DEVELOPMENTAL DYNAMICS 196: 133 (1993). (10.1002/aja.1001960207) / DEVELOPMENTAL DYNAMICS (1993)
LIN, H.Y., CELL 68: 1 (1992). (10.1016/0092-8674(92)90198-L) / CELL (1992)
LIN, H.Y., TRENDS CELL BIOL 3: 14 (1993). (10.1016/0962-8924(93)90195-7) / TRENDS CELL BIOL (1993)
MATHEWS, L.S., EXPRESSION CLONING OF AN ACTIVIN RECEPTOR, A PREDICTED TRANSMEMBRANE SERINE KINASE, CELL 65: 973 (1991). (10.1016/0092-8674(91)90549-E) / CELL (1991)
MATSUZAKI, K, A WIDELY EXPRESSED TRANSMEMBRANE SERINE THREONINE KINASE THAT DOES NOT BIND ACTIVIN, INHIBIN, TRANSFORMING GROWTH-FACTOR-BETA, OR BONE MORPHOGENIC FACTOR, JOURNAL OF BIOLOGICAL CHEMISTRY 268: 12719 (1993). (10.1016/S0021-9258(18)31447-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1993)
10.1126/science.8140412
TSUCHIDA, K, CLONING AND CHARACTERIZATION OF A TRANSMEMBRANE SERINE KINASE THAT ACTS AS AN ACTIVIN TYPE-I RECEPTOR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 90: 11242 (1993). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1993)
VANDUYNE, G.D., ATOMIC-STRUCTURE OF THE RAPAMYCIN HUMAN IMMUNOPHILIN FKBP-12 COMPLEX, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 113: 7433 (1991). (10.1021/ja00019a057) / JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (1991)
WANG T unpublished data.
WITTENBERG, C, CONTROL OF THE YEAST-CELL CYCLE IS ASSOCIATED WITH ASSEMBLY DISASSEMBLY OF THE CDC28 PROTEIN-KINASE COMPLEX, CELL 54: 1061 (1988). (10.1016/0092-8674(88)90121-3) / CELL (1988)
10.1016/0092-8674(92)90395-S
YAMAZAKI, Y, DNA SEQUENCE 3: 297 (1993). (10.3109/10425179309020827) / DNA SEQUENCE (1993)
ZERVOS, A.S., MXI1, A PROTEIN THAT SPECIFICALLY INTERACTS WITH MAX TO BIND MYC-MAX RECOGNITION SITES, CELL 72: 223 (1993). (10.1016/0092-8674(93)90662-A) / CELL (1993)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 8:01 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:03 p.m.)
Indexed	1 week, 6 days ago (Aug. 22, 2025, 12:47 a.m.)
Issued	31 years, 1 month ago (July 29, 1994)
Published	31 years, 1 month ago (July 29, 1994)
Published Print	31 years, 1 month ago (July 29, 1994)

Funders 0

None

BibTeX

@article{Wang_1994, title={Specific Interaction of Type I Receptors of the TGF-β Family with the Immunophilin FKBP-12}, volume={265}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7518616}, DOI={10.1126/science.7518616}, number={5172}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Wang, Tongwen and Donahoe, Patricia K. and Zervos, Antonis S.}, year={1994}, month=jul, pages={674–676} }

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