DOI: 10.1126/science.7423203. Antibody Targeting of Liposomes: Cell Specificity Obtained by Conjugation of F(ab′) <sub>2</sub> to Vesicle Surface

Antibody Targeting of Liposomes: Cell Specificity Obtained by Conjugation of F(ab′) 2 to Vesicle Surface

10.1126/science.7423203

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A method devised for conjugating liposomes with protein resulted in the binding of up to 200 micrograms of immunoglobulin G per micromole of lipid. The coupling of antibody to human erythrocyte F(ab′) 2 in vesicles (140 molecules per vesicle) by this method caused a 200-fold increase in the binding of vesicles to human erythrocytes and resulted in about 80 percent of the vesicle lipid and contents being associated with cells.

Bibliography

Heath, T. D., Fraley, R. T., & Papahdjopoulos, D. (1980). Antibody Targeting of Liposomes: Cell Specificity Obtained by Conjugation of F(abâ²) 2 to Vesicle Surface. Science, 210(4469), 539â541.

Authors 3

Timothy D. Heath (first)
Robert T. Fraley (additional)
Demetrios Papahdjopoulos (additional)

References 15 Referenced 206

BLIGH, E.G., A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION, CANADIAN JOURNAL OF BIOCHEMISTRY AND PHYSIOLOGY 37: 911 (1959). (10.1139/y59-099) / CANADIAN JOURNAL OF BIOCHEMISTRY AND PHYSIOLOGY (1959)
DUNNICK, J.K., VESICLE INTERACTIONS WITH POLYAMINO ACIDS AND ANTIBODY - INVITRO AND INVIVO STUDIES, JOURNAL OF NUCLEAR MEDICINE 16: 483 (1975). / JOURNAL OF NUCLEAR MEDICINE (1975)
GREGORIADIS, G, HOMING OF LIPOSOMES TO TARGET-CELLS, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 65: 537 (1975). (10.1016/S0006-291X(75)80180-X) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1975)
Heath, T. D., Biochimica et Biophysica Acta (BBA) - Biomembranes 640: 66 (1981). (10.1016/0005-2736(81)90532-0) / Biochimica et Biophysica Acta (BBA) - Biomembranes (1981)
HEATH, T.D., COVALENT ATTACHMENT OF HORSERADISH-PEROXIDASE TO THE OUTER SURFACE OF LIPOSOMES, BIOCHIMICA ET BIOPHYSICA ACTA 599: 42 (1980). (10.1016/0005-2736(80)90055-3) / BIOCHIMICA ET BIOPHYSICA ACTA (1980)
JENTOFT, N, LABELING OF PROTEINS BY REDUCTIVE METHYLATION USING SODIUM CYANOBOROHYDRIDE, JOURNAL OF BIOLOGICAL CHEMISTRY 254: 4359 (1979). (10.1016/S0021-9258(17)30016-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1979)
JULIANO, R.L., LECTIN-MEDIATED ATTACHMENT OF GLYCOPROTEIN-BEARING LIPOSOMES TO CELLS, NATURE 261: 235 (1976). (10.1038/261235a0) / NATURE (1976)
LESERMAN, L.D., BINDING OF ANTIGEN-BEARING FLUORESCENT LIPOSOMES TO THE MURINE MYELOMA TUMOR MOPC 315, JOURNAL OF IMMUNOLOGY 122: 585 (1979). (10.4049/jimmunol.122.2.585) / JOURNAL OF IMMUNOLOGY (1979)
MAGEE, W.E., ANN NY ACAD SCI 308: 308 (1978). (10.1111/j.1749-6632.1978.tb22032.x) / ANN NY ACAD SCI (1978)
Papahadjopoulos, D., Annual Reports in Medicinal Chemistry 14: 250 (1979). (10.1016/S0065-7743(08)61369-X) / Annual Reports in Medicinal Chemistry (1979)
SZOKA, F, PROCEDURE FOR PREPARATION OF LIPOSOMES WITH LARGE INTERNAL AQUEOUS SPACE AND HIGH CAPTURE BY REVERSE-PHASE EVAPORATION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 75: 4194 (1978). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1978)
TORCHILIN, V.P., PRESERVATION OF ANTIMYOSIN ANTIBODY-ACTIVITY AFTER COVALENT COUPLING TO LIPOSOMES, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 89: 1114 (1979). (10.1016/0006-291X(79)92123-5) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1979)
TYRELL, D.A., BIOCHIM BIOPHYS ACTA 457: 259 (1976). (10.1016/0304-4157(76)90002-2) / BIOCHIM BIOPHYS ACTA (1976)
WEINSTEIN, J.N., ANTIBODY-MEDIATED TARGETING OF LIPOSOMES - BINDING TO LYMPHOCYTES DOES NOT ENSURE INCORPORATION OF VESICLE CONTENTS INTO CELLS, BIOCHIMICA ET BIOPHYSICA ACTA 509: 272 (1978). (10.1016/0005-2736(78)90047-0) / BIOCHIMICA ET BIOPHYSICA ACTA (1978)
WEISSMANN, G, GENERAL METHOD FOR INTRODUCTION OF ENZYMES BY MEANS OF IMMUNOGLOBULIN-COATED LIPOSOMES, INTO LYSOSOMES OF DEFICIENT CELLS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 72: 88 (1975). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1975)

Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 1:45 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:32 p.m.)
Indexed	1 day, 7 hours ago (Sept. 4, 2025, 9:24 a.m.)
Issued	44 years, 10 months ago (Oct. 31, 1980)
Published	44 years, 10 months ago (Oct. 31, 1980)
Published Print	44 years, 10 months ago (Oct. 31, 1980)

Funders 0

None

BibTeX

@article{Heath_1980, title={Antibody Targeting of Liposomes: Cell Specificity Obtained by Conjugation of F(ab′) 2 to Vesicle Surface}, volume={210}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7423203}, DOI={10.1126/science.7423203}, number={4469}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Heath, Timothy D. and Fraley, Robert T. and Papahdjopoulos, Demetrios}, year={1980}, month=oct, pages={539–541} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        9,
        4
      ]
    ],
    "date-time": "2025-09-04T13:24:59Z",
    "timestamp": 1756992299086
  },
  "reference-count": 15,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "4469",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1980,
        10,
        31
      ]
    ]
  },
  "abstract": "<jats:p>\n            A method devised for conjugating liposomes with protein resulted in the binding of up to 200 micrograms of immunoglobulin G per micromole of lipid. The coupling of antibody to human erythrocyte F(ab\u2032)\n            <jats:sub>2</jats:sub>\n            in vesicles (140 molecules per vesicle) by this method caused a 200-fold increase in the binding of vesicles to human erythrocytes and resulted in about 80 percent of the vesicle lipid and contents being associated with cells.\n          </jats:p>",
  "DOI": "10.1126/science.7423203",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        10,
        5
      ]
    ],
    "date-time": "2006-10-05T17:45:58Z",
    "timestamp": 1160070358000
  },
  "page": "539-541",
  "source": "Crossref",
  "is-referenced-by-count": 206,
  "title": "Antibody Targeting of Liposomes: Cell Specificity Obtained by Conjugation of F(ab\u2032)\n            <sub>2</sub>\n            to Vesicle Surface",
  "prefix": "10.1126",
  "volume": "210",
  "author": [
    {
      "given": "Timothy D.",
      "family": "Heath",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Division of Biology, Chester Beatty Research Institute, London, England"
        },
        {
          "name": "Cancer Research Institute, University of California, San Francisco 94143"
        }
      ]
    },
    {
      "given": "Robert T.",
      "family": "Fraley",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Cancer Research Institute, University of California, San Francisco 94143"
        }
      ]
    },
    {
      "given": "Demetrios",
      "family": "Papahdjopoulos",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Pharmacology, Cancer Research Institute, University of California, San Francisco 94143"
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_2_1_1_1",
      "doi-asserted-by": "crossref",
      "first-page": "911",
      "DOI": "10.1139/y59-099",
      "volume": "37",
      "year": "1959",
      "unstructured": "BLIGH, E.G., A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATION, CANADIAN JOURNAL OF BIOCHEMISTRY AND PHYSIOLOGY 37: 911 (1959).",
      "journal-title": "CANADIAN JOURNAL OF BIOCHEMISTRY AND PHYSIOLOGY"
    },
    {
      "key": "e_1_2_1_2_1",
      "first-page": "483",
      "volume": "16",
      "year": "1975",
      "unstructured": "DUNNICK, J.K., VESICLE INTERACTIONS WITH POLYAMINO ACIDS AND ANTIBODY - INVITRO AND INVIVO STUDIES, JOURNAL OF NUCLEAR MEDICINE 16: 483 (1975).",
      "journal-title": "JOURNAL OF NUCLEAR MEDICINE"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "crossref",
      "first-page": "537",
      "DOI": "10.1016/S0006-291X(75)80180-X",
      "volume": "65",
      "year": "1975",
      "unstructured": "GREGORIADIS, G, HOMING OF LIPOSOMES TO TARGET-CELLS, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 65: 537 (1975).",
      "journal-title": "BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "crossref",
      "first-page": "66",
      "DOI": "10.1016/0005-2736(81)90532-0",
      "volume": "640",
      "year": "1981",
      "unstructured": "Heath, T. D., Biochimica et Biophysica Acta (BBA) - Biomembranes 640: 66 (1981).",
      "journal-title": "Biochimica et Biophysica Acta (BBA) - Biomembranes"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "crossref",
      "first-page": "42",
      "DOI": "10.1016/0005-2736(80)90055-3",
      "volume": "599",
      "year": "1980",
      "unstructured": "HEATH, T.D., COVALENT ATTACHMENT OF HORSERADISH-PEROXIDASE TO THE OUTER SURFACE OF LIPOSOMES, BIOCHIMICA ET BIOPHYSICA ACTA 599: 42 (1980).",
      "journal-title": "BIOCHIMICA ET BIOPHYSICA ACTA"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "crossref",
      "first-page": "4359",
      "DOI": "10.1016/S0021-9258(17)30016-9",
      "volume": "254",
      "year": "1979",
      "unstructured": "JENTOFT, N, LABELING OF PROTEINS BY REDUCTIVE METHYLATION USING SODIUM CYANOBOROHYDRIDE, JOURNAL OF BIOLOGICAL CHEMISTRY 254: 4359 (1979).",
      "journal-title": "JOURNAL OF BIOLOGICAL CHEMISTRY"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "crossref",
      "first-page": "235",
      "DOI": "10.1038/261235a0",
      "volume": "261",
      "year": "1976",
      "unstructured": "JULIANO, R.L., LECTIN-MEDIATED ATTACHMENT OF GLYCOPROTEIN-BEARING LIPOSOMES TO CELLS, NATURE 261: 235 (1976).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "crossref",
      "first-page": "585",
      "DOI": "10.4049/jimmunol.122.2.585",
      "volume": "122",
      "year": "1979",
      "unstructured": "LESERMAN, L.D., BINDING OF ANTIGEN-BEARING FLUORESCENT LIPOSOMES TO THE MURINE MYELOMA TUMOR MOPC 315, JOURNAL OF IMMUNOLOGY 122: 585 (1979).",
      "journal-title": "JOURNAL OF IMMUNOLOGY"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "crossref",
      "first-page": "308",
      "DOI": "10.1111/j.1749-6632.1978.tb22032.x",
      "volume": "308",
      "year": "1978",
      "unstructured": "MAGEE, W.E., ANN NY ACAD SCI 308: 308 (1978).",
      "journal-title": "ANN NY ACAD SCI"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "crossref",
      "first-page": "250",
      "DOI": "10.1016/S0065-7743(08)61369-X",
      "volume": "14",
      "year": "1979",
      "unstructured": "Papahadjopoulos, D., Annual Reports in Medicinal Chemistry 14: 250 (1979).",
      "journal-title": "Annual Reports in Medicinal Chemistry"
    },
    {
      "key": "e_1_2_1_11_1",
      "first-page": "4194",
      "volume": "75",
      "year": "1978",
      "unstructured": "SZOKA, F, PROCEDURE FOR PREPARATION OF LIPOSOMES WITH LARGE INTERNAL AQUEOUS SPACE AND HIGH CAPTURE BY REVERSE-PHASE EVAPORATION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 75: 4194 (1978).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "crossref",
      "first-page": "1114",
      "DOI": "10.1016/0006-291X(79)92123-5",
      "volume": "89",
      "year": "1979",
      "unstructured": "TORCHILIN, V.P., PRESERVATION OF ANTIMYOSIN ANTIBODY-ACTIVITY AFTER COVALENT COUPLING TO LIPOSOMES, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 89: 1114 (1979).",
      "journal-title": "BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "crossref",
      "first-page": "259",
      "DOI": "10.1016/0304-4157(76)90002-2",
      "volume": "457",
      "year": "1976",
      "unstructured": "TYRELL, D.A., BIOCHIM BIOPHYS ACTA 457: 259 (1976).",
      "journal-title": "BIOCHIM BIOPHYS ACTA"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "crossref",
      "first-page": "272",
      "DOI": "10.1016/0005-2736(78)90047-0",
      "volume": "509",
      "year": "1978",
      "unstructured": "WEINSTEIN, J.N., ANTIBODY-MEDIATED TARGETING OF LIPOSOMES - BINDING TO LYMPHOCYTES DOES NOT ENSURE INCORPORATION OF VESICLE CONTENTS INTO CELLS, BIOCHIMICA ET BIOPHYSICA ACTA 509: 272 (1978).",
      "journal-title": "BIOCHIMICA ET BIOPHYSICA ACTA"
    },
    {
      "key": "e_1_2_1_15_1",
      "first-page": "88",
      "volume": "72",
      "year": "1975",
      "unstructured": "WEISSMANN, G, GENERAL METHOD FOR INTRODUCTION OF ENZYMES BY MEANS OF IMMUNOGLOBULIN-COATED LIPOSOMES, INTO LYSOSOMES OF DEFICIENT CELLS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 72: 88 (1975).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.7423203",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        15
      ]
    ],
    "date-time": "2024-01-15T04:32:47Z",
    "timestamp": 1705293167000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.7423203"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1980,
        10,
        31
      ]
    ]
  },
  "references-count": 15,
  "journal-issue": {
    "issue": "4469",
    "published-print": {
      "date-parts": [
        [
          1980,
          10,
          31
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.7423203"
  ],
  "URL": "http://dx.doi.org/10.1126/science.7423203",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1980,
        10,
        31
      ]
    ]
  }
}