DOI: 10.1126/science.7384789. Therapy of Spontaneous Metastases by Intravenous Injection of Liposomes Containing Lymphokines

Therapy of Spontaneous Metastases by Intravenous Injection of Liposomes Containing Lymphokines

10.1126/science.7384789

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Mice of two different strains were injected subcutaneously with spontaneously metastasizing syngeneic melanomas. After 4 to 6 weeks, the local tumors were removed and, 3 days after surgery, treatment of the metastases was initiated. The treatment consisted of intravenous injections of liposomes containing lymphokines or control supernatant fluids. Liposomes were injected twice weekly for 3 weeks, and the mice were killed 2 weeks later. Seventy-three percent of the mice injected with liposomes containing lymphokines were free of metastases, whereas only 10 percent of the mice treated with control liposomes were tumor-free. These experiments suggest that this form of therapy may provide a valuable addition to the more conventional approaches to the eradication of cancer metastases.

Bibliography

Fidler, I. J. (1980). Therapy of Spontaneous Metastases by Intravenous Injection of Liposomes Containing Lymphokines. Science, 208(4451), 1469â1471.

Authors 1

Isaiah J. Fidler (first)

References 16 Referenced 151

ALLISON, A.C., MODE OF ACTION OF IMMUNOLOGICAL ADJUVANTS, JOURNAL OF THE RETICULOENDOTHELIAL SOCIETY 26: 619 (1979). / JOURNAL OF THE RETICULOENDOTHELIAL SOCIETY (1979)
Fidler I. J. Cancer Chemo- and Immunopharmacology (1980).
FIDLER, I.J., INHIBITION OF PULMONARY METASTASIS BY INTRAVENOUS-INJECTION OF SPECIFICALLY ACTIVATED MACROPHAGES, CANCER RESEARCH 34: 1074 (1974). / CANCER RESEARCH (1974)
FIDLER, I.J., TUMORICIDAL PROPERTIES OF MOUSE MACROPHAGES ACTIVATED WITH MEDIATORS FROM RAT LYMPHOCYTES STIMULATED WITH CONCANAVALIN-A, CANCER RESEARCH 36: 3608 (1976). / CANCER RESEARCH (1976)
FINKELSTEIN, M, INTRODUCTION OF ENZYMES INTO CELLS BY MEANS OF LIPOSOMES, JOURNAL OF LIPID RESEARCH 19: 289 (1978). (10.1016/S0022-2275(20)41300-8) / JOURNAL OF LIPID RESEARCH (1978)
HART, I.R., SELECTION AND CHARACTERIZATION OF AN INVASIVE VARIANT OF THE B-16 MELANOMA, AMERICAN JOURNAL OF PATHOLOGY 97: 587 (1979). / AMERICAN JOURNAL OF PATHOLOGY (1979)
Hart, I. R., Cancer Immunology, Immunotherapy 10: 157 (1981). / Cancer Immunology, Immunotherapy (1981)
KERBEL, R.S., IMPLICATIONS OF IMMUNOLOGICAL HETEROGENEITY OF TUMORS, NATURE 280: 358 (1979). (10.1038/280358a0) / NATURE (1979)
KRIPKE, M.L., PRODUCTION OF SPECIFIC MACROPHAGE ACTIVATING FACTOR BY LYMPHOCYTES FROM TUMOR-BEARING MICE, CELLULAR IMMUNOLOGY 30: 341 (1977). (10.1016/0008-8749(77)90077-6) / CELLULAR IMMUNOLOGY (1977)
KRIPKE, M.L., SPECULATIONS ON THE ROLE OF ULTRAVIOLET-RADIATION IN THE DEVELOPMENT OF MALIGNANT-MELANOMA, JOURNAL OF THE NATIONAL CANCER INSTITUTE 63: 541 (1979). (10.1093/jnci/63.3.541) / JOURNAL OF THE NATIONAL CANCER INSTITUTE (1979)
LIOTTA, L.A., REDUCTION OF TUMOR-CELL ENTRY INTO VESSELS BY BCG-ACTIVATED MACROPHAGES, BRITISH JOURNAL OF CANCER 36: 639 (1977). (10.1038/bjc.1977.242) / BRITISH JOURNAL OF CANCER (1977)
PAGANO, R.E., INTERACTIONS OF LIPOSOMES WITH MAMMALIAN-CELLS, ANNUAL REVIEW OF BIOPHYSICS AND BIOENGINEERING 7: 435 (1978). (10.1146/annurev.bb.07.060178.002251) / ANNUAL REVIEW OF BIOPHYSICS AND BIOENGINEERING (1978)
POSTE, G, ACTIVATION OF TUMORICIDAL PROPERTIES IN MOUSE MACROPHAGES BY LYMPHOKINES ENCAPSULATED IN LIPOSOMES, CANCER RESEARCH 39: 881 (1979). / CANCER RESEARCH (1979)
POSTE, G, PATHOGENESIS OF CANCER METASTASIS, NATURE 283: 139 (1980). (10.1038/283139a0) / NATURE (1980)
POSTE, G, LIPID VESICLES AS CARRIERS FOR INTRODUCING MATERIALS INTO CULTURED-CELLS - INFLUENCE OF VESICLE LIPID-COMPOSITION ON MECHANISM(S) OF VESICLE INCORPORATION INTO CELLS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 73: 1603 (1976). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1976)
SIEGEL, S, NONPARAMETRIC STAT B: 116 (1956). / NONPARAMETRIC STAT B (1956)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 1:45 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 10:45 p.m.)
Indexed	3 weeks, 5 days ago (Aug. 1, 2025, 11:58 p.m.)
Issued	45 years, 2 months ago (June 27, 1980)
Published	45 years, 2 months ago (June 27, 1980)
Published Print	45 years, 2 months ago (June 27, 1980)

Funders 0

None

BibTeX

@article{Fidler_1980, title={Therapy of Spontaneous Metastases by Intravenous Injection of Liposomes Containing Lymphokines}, volume={208}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7384789}, DOI={10.1126/science.7384789}, number={4451}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Fidler, Isaiah J.}, year={1980}, month=jun, pages={1469–1471} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        2
      ]
    ],
    "date-time": "2025-08-02T03:58:40Z",
    "timestamp": 1754107120171
  },
  "reference-count": 16,
  "publisher": "American Association for the Advancement of Science (AAAS)",
  "issue": "4451",
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1980,
        6,
        27
      ]
    ]
  },
  "abstract": "<jats:p>Mice of two different strains were injected subcutaneously with spontaneously metastasizing syngeneic melanomas. After 4 to 6 weeks, the local tumors were removed and, 3 days after surgery, treatment of the metastases was initiated. The treatment consisted of intravenous injections of liposomes containing lymphokines or control supernatant fluids. Liposomes were injected twice weekly for 3 weeks, and the mice were killed 2 weeks later. Seventy-three percent of the mice injected with liposomes containing lymphokines were free of metastases, whereas only 10 percent of the mice treated with control liposomes were tumor-free. These experiments suggest that this form of therapy may provide a valuable addition to the more conventional approaches to the eradication of cancer metastases.</jats:p>",
  "DOI": "10.1126/science.7384789",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        10,
        5
      ]
    ],
    "date-time": "2006-10-05T17:45:58Z",
    "timestamp": 1160070358000
  },
  "page": "1469-1471",
  "source": "Crossref",
  "is-referenced-by-count": 151,
  "title": "Therapy of Spontaneous Metastases by Intravenous Injection of Liposomes Containing Lymphokines",
  "prefix": "10.1126",
  "volume": "208",
  "author": [
    {
      "given": "Isaiah J.",
      "family": "Fidler",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Cancer Metastasis and Treatment Laboratory, Frederick Cancer Research Center, Frederick, Maryland 21701"
        }
      ]
    }
  ],
  "member": "221",
  "reference": [
    {
      "key": "e_1_2_1_1_1",
      "first-page": "619",
      "volume": "26",
      "year": "1979",
      "unstructured": "ALLISON, A.C., MODE OF ACTION OF IMMUNOLOGICAL ADJUVANTS, JOURNAL OF THE RETICULOENDOTHELIAL SOCIETY 26: 619 (1979).",
      "journal-title": "JOURNAL OF THE RETICULOENDOTHELIAL SOCIETY"
    },
    {
      "key": "e_1_2_1_2_1",
      "unstructured": "Fidler I. J. Cancer Chemo- and Immunopharmacology (1980)."
    },
    {
      "key": "e_1_2_1_3_1",
      "first-page": "1074",
      "volume": "34",
      "year": "1974",
      "unstructured": "FIDLER, I.J., INHIBITION OF PULMONARY METASTASIS BY INTRAVENOUS-INJECTION OF SPECIFICALLY ACTIVATED MACROPHAGES, CANCER RESEARCH 34: 1074 (1974).",
      "journal-title": "CANCER RESEARCH"
    },
    {
      "key": "e_1_2_1_4_1",
      "first-page": "3608",
      "volume": "36",
      "year": "1976",
      "unstructured": "FIDLER, I.J., TUMORICIDAL PROPERTIES OF MOUSE MACROPHAGES ACTIVATED WITH MEDIATORS FROM RAT LYMPHOCYTES STIMULATED WITH CONCANAVALIN-A, CANCER RESEARCH 36: 3608 (1976).",
      "journal-title": "CANCER RESEARCH"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "crossref",
      "first-page": "289",
      "DOI": "10.1016/S0022-2275(20)41300-8",
      "volume": "19",
      "year": "1978",
      "unstructured": "FINKELSTEIN, M, INTRODUCTION OF ENZYMES INTO CELLS BY MEANS OF LIPOSOMES, JOURNAL OF LIPID RESEARCH 19: 289 (1978).",
      "journal-title": "JOURNAL OF LIPID RESEARCH"
    },
    {
      "key": "e_1_2_1_6_1",
      "first-page": "587",
      "volume": "97",
      "year": "1979",
      "unstructured": "HART, I.R., SELECTION AND CHARACTERIZATION OF AN INVASIVE VARIANT OF THE B-16 MELANOMA, AMERICAN JOURNAL OF PATHOLOGY 97: 587 (1979).",
      "journal-title": "AMERICAN JOURNAL OF PATHOLOGY"
    },
    {
      "key": "e_1_2_1_7_1",
      "first-page": "157",
      "volume": "10",
      "year": "1981",
      "unstructured": "Hart, I. R., Cancer Immunology, Immunotherapy 10: 157 (1981).",
      "journal-title": "Cancer Immunology, Immunotherapy"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "crossref",
      "first-page": "358",
      "DOI": "10.1038/280358a0",
      "volume": "280",
      "year": "1979",
      "unstructured": "KERBEL, R.S., IMPLICATIONS OF IMMUNOLOGICAL HETEROGENEITY OF TUMORS, NATURE 280: 358 (1979).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "crossref",
      "first-page": "341",
      "DOI": "10.1016/0008-8749(77)90077-6",
      "volume": "30",
      "year": "1977",
      "unstructured": "KRIPKE, M.L., PRODUCTION OF SPECIFIC MACROPHAGE ACTIVATING FACTOR BY LYMPHOCYTES FROM TUMOR-BEARING MICE, CELLULAR IMMUNOLOGY 30: 341 (1977).",
      "journal-title": "CELLULAR IMMUNOLOGY"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "crossref",
      "first-page": "541",
      "DOI": "10.1093/jnci/63.3.541",
      "volume": "63",
      "year": "1979",
      "unstructured": "KRIPKE, M.L., SPECULATIONS ON THE ROLE OF ULTRAVIOLET-RADIATION IN THE DEVELOPMENT OF MALIGNANT-MELANOMA, JOURNAL OF THE NATIONAL CANCER INSTITUTE 63: 541 (1979).",
      "journal-title": "JOURNAL OF THE NATIONAL CANCER INSTITUTE"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "crossref",
      "first-page": "639",
      "DOI": "10.1038/bjc.1977.242",
      "volume": "36",
      "year": "1977",
      "unstructured": "LIOTTA, L.A., REDUCTION OF TUMOR-CELL ENTRY INTO VESSELS BY BCG-ACTIVATED MACROPHAGES, BRITISH JOURNAL OF CANCER 36: 639 (1977).",
      "journal-title": "BRITISH JOURNAL OF CANCER"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "crossref",
      "first-page": "435",
      "DOI": "10.1146/annurev.bb.07.060178.002251",
      "volume": "7",
      "year": "1978",
      "unstructured": "PAGANO, R.E., INTERACTIONS OF LIPOSOMES WITH MAMMALIAN-CELLS, ANNUAL REVIEW OF BIOPHYSICS AND BIOENGINEERING 7: 435 (1978).",
      "journal-title": "ANNUAL REVIEW OF BIOPHYSICS AND BIOENGINEERING"
    },
    {
      "key": "e_1_2_1_13_1",
      "first-page": "881",
      "volume": "39",
      "year": "1979",
      "unstructured": "POSTE, G, ACTIVATION OF TUMORICIDAL PROPERTIES IN MOUSE MACROPHAGES BY LYMPHOKINES ENCAPSULATED IN LIPOSOMES, CANCER RESEARCH 39: 881 (1979).",
      "journal-title": "CANCER RESEARCH"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "crossref",
      "first-page": "139",
      "DOI": "10.1038/283139a0",
      "volume": "283",
      "year": "1980",
      "unstructured": "POSTE, G, PATHOGENESIS OF CANCER METASTASIS, NATURE 283: 139 (1980).",
      "journal-title": "NATURE"
    },
    {
      "key": "e_1_2_1_15_1",
      "first-page": "1603",
      "volume": "73",
      "year": "1976",
      "unstructured": "POSTE, G, LIPID VESICLES AS CARRIERS FOR INTRODUCING MATERIALS INTO CULTURED-CELLS - INFLUENCE OF VESICLE LIPID-COMPOSITION ON MECHANISM(S) OF VESICLE INCORPORATION INTO CELLS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 73: 1603 (1976).",
      "journal-title": "PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"
    },
    {
      "key": "e_1_2_1_16_1",
      "first-page": "116",
      "year": "1956",
      "unstructured": "SIEGEL, S, NONPARAMETRIC STAT B: 116 (1956).",
      "journal-title": "NONPARAMETRIC STAT B"
    }
  ],
  "container-title": "Science",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://www.science.org/doi/pdf/10.1126/science.7384789",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        1,
        15
      ]
    ],
    "date-time": "2024-01-15T03:45:31Z",
    "timestamp": 1705290331000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://www.science.org/doi/10.1126/science.7384789"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1980,
        6,
        27
      ]
    ]
  },
  "references-count": 16,
  "journal-issue": {
    "issue": "4451",
    "published-print": {
      "date-parts": [
        [
          1980,
          6,
          27
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1126/science.7384789"
  ],
  "URL": "http://dx.doi.org/10.1126/science.7384789",
  "relation": {},
  "ISSN": [
    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1980,
        6,
        27
      ]
    ]
  }
}