DOI: 10.1126/science.7201167. Intracellular Oxidation-Reduction State Measured in Situ by a Multichannel Fiber-Optic Surface Fluorometer [Penn]

Intracellular Oxidation-Reduction State Measured in Situ by a Multichannel Fiber-Optic Surface Fluorometer

10.1126/science.7201167

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The principles of the measurement in vivo of the oxidation-reduction state of intramitochrondrial pyridine nucleotides were used in establishing a multichannel fluorometer-reflectometer. This approach made possible the study of changes of mitochrondrial redox states in four different organs (brain, liver, kidney, and testis) of the same animal, as well as the monitoring of four different cortical areas of the same brain hemisphere. In the measurement of reduced nicotinamide adenine dinucleotide fluorescence, oximetric and movement artifacts are negligible, but blood volume changes and tissue absorption properties are a source of error. The corrected fluorescence is obtained by subtracting the reflectance from the fluorescence signal in 1:1 ratio. During graded hypoxia, the corrected fluorescence showed a gradual increase and was maximal during anoxia in all four organs tested.

Bibliography

Mayevsky, A., & Chance, B. (1982). Intracellular Oxidation-Reduction State Measured in Situ by a Multichannel Fiber-Optic Surface Fluorometer. Science, 217(4559), 537â540.

Authors 2

Avraham Mayevsky (first)
Britton Chance (additional)

References 26 Referenced 190

AUBERT, X, P R SOC LOND B 160: 211 (1963). / P R SOC LOND B (1963)
CHANCE, B, VERSATILE TIME-SHARING MULTICHANNEL SPECTROPHOTOMETER, REFLECTOMETER, AND FLUOROMETER, ANALYTICAL BIOCHEMISTRY 66: 498 (1975). (10.1016/0003-2697(75)90617-X) / ANALYTICAL BIOCHEMISTRY (1975)
Chance, B., Journal of Biological Chemistry 217: 383 (1955). (10.1016/S0021-9258(19)57189-7) / Journal of Biological Chemistry (1955)
CHANCE, B, CORRELATION OF OXIDATION-REDUCTION CHANGES OF INTRACELLULAR REDUCED PYRIDINE NUCLEOTIDE AND CHANGES IN ELECTROENCEPHALOGRAM OF RAT IN ANOXIA, NATURE 195: 956 (1962). (10.1038/195956a0) / NATURE (1962)
CHANCE, B, OXYGEN TRANSPORT TIS 1: 277 (1973). / OXYGEN TRANSPORT TIS (1973)
10.1126/science.137.3529.499
DORA, E, REACTIVITY OF THE CEREBROCORTICAL VASCULATURE AND ENERGY-METABOLISM TO DIRECT CORTICAL STIMULATION IN HEMORRHAGIC-SHOCK, ACTA PHYSIOLOGICA ACADEMIAE SCIENTIARUM HUNGARICAE 54: 347 (1979). / ACTA PHYSIOLOGICA ACADEMIAE SCIENTIARUM HUNGARICAE (1979)
JOBSIS, F.F., EXTRA PULMONARY MANI: 63 (1978). / EXTRA PULMONARY MANI (1978)
JOBSIS, F.F., INTRACELLULAR REDOX CHANGES IN FUNCTIONING CEREBRAL CORTEX .1. METABOLIC EFFECTS OF EPILEPTIFORM ACTIVITY, JOURNAL OF NEUROPHYSIOLOGY 34: 735 (1971). (10.1152/jn.1971.34.5.735) / JOURNAL OF NEUROPHYSIOLOGY (1971)
KEDEM, J, AN EXPERIMENTAL APPROACH FOR EVALUATION OF THE O2 BALANCE IN LOCAL MYOCARDIAL REGIONS INVIVO, QUARTERLY JOURNAL OF EXPERIMENTAL PHYSIOLOGY AND COGNATE MEDICAL SCIENCES 66: 501 (1981). / QUARTERLY JOURNAL OF EXPERIMENTAL PHYSIOLOGY AND COGNATE MEDICAL SCIENCES (1981)
KOVACH, AGB, OXYGEN PHYSL FUNCTIO: 111 (1977). / OXYGEN PHYSL FUNCTIO (1977)
10.1126/science.223243
LAMANNA, J.C., EFFECT OF OUABAIN AND PHENOBARBITAL ON OXIDATIVE METABOLIC-ACTIVITY ASSOCIATED WITH SPREADING CORTICAL DEPRESSION IN CATS, BRAIN RESEARCH 88: 145 (1975). (10.1016/0006-8993(75)90963-4) / BRAIN RESEARCH (1975)
MAYEVSKY, A, BIBL ANAT 15: 307 (1977). / BIBL ANAT (1977)
MAYEVSKY, A, METABOLIC RESPONSES OF AWAKE CEREBRAL-CORTEX TO ANOXIA HYPOXIA SPREADING DEPRESSION AND EPILEPTIFORM ACTIVITY, BRAIN RESEARCH 98: 149 (1975). (10.1016/0006-8993(75)90515-6) / BRAIN RESEARCH (1975)
MAYEVSKY, A, THE EFFECTS OF UNILATERAL CAROTID OCCLUSION ON THE RESPONSES TO DECAPITATION IN THE GERBIL BRAIN, BRAIN RESEARCH 206: 155 (1981). (10.1016/0006-8993(81)90108-6) / BRAIN RESEARCH (1981)
MAYEVSKY, A, FACTORS AFFECTING THE OXYGEN BALANCE IN THE AWAKE CEREBRAL-CORTEX EXPOSED TO SPREADING DEPRESSION, BRAIN RESEARCH 236: 93 (1982). (10.1016/0006-8993(82)90037-3) / BRAIN RESEARCH (1982)
MAYEVSKY, A, FRONTIERS BIOENERGET: 1467 (1978). / FRONTIERS BIOENERGET (1978)
MAYEVSKY, A, THE INTERRELATION BETWEEN BRAIN PO2 AND NADH OXIDATION-REDUCTION STATE IN THE GERBIL, JOURNAL OF NEUROSCIENCE RESEARCH 5: 173 (1980). (10.1002/jnr.490050302) / JOURNAL OF NEUROSCIENCE RESEARCH (1980)
Mayevsky, A., Neurological Research 3: 307 (1981). (10.1080/01616412.1981.11758585) / Neurological Research (1981)
MAYEVSKY, A, NEUROL RES 1: 213 (1980). (10.1080/01616412.1980.11739554) / NEUROL RES (1980)
MAYEVSKY, A, OXYGEN TRANSPORT TIS 1: 239 (1973). / OXYGEN TRANSPORT TIS (1973)
MAYEVSKY, A, OXYGEN TRANSPORT TIS 2: 307 (1976). / OXYGEN TRANSPORT TIS (1976)
MAYEVSKY, A, OXYGEN TRANSPORT TIS 3: 761 (1978). / OXYGEN TRANSPORT TIS (1978)
MAYEVSKY A unpublished data.
ROSENTHAL, M, INTRACELLULAR REDOX CHANGES IN FUNCTIONING CEREBRAL CORTEX .2. EFFECTS OF DIRECT CORTICAL STIMULATION, JOURNAL OF NEUROPHYSIOLOGY 34: 750 (1971). (10.1152/jn.1971.34.5.750) / JOURNAL OF NEUROPHYSIOLOGY (1971)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 2:30 p.m.)
Deposited	1 year, 7 months ago (Jan. 15, 2024, 12:10 a.m.)
Indexed	6 days, 19 hours ago (Aug. 23, 2025, 9:18 p.m.)
Issued	43 years ago (Aug. 6, 1982)
Published	43 years ago (Aug. 6, 1982)
Published Print	43 years ago (Aug. 6, 1982)

Funders 0

None

BibTeX

@article{Mayevsky_1982, title={Intracellular Oxidation-Reduction State Measured in Situ by a Multichannel Fiber-Optic Surface Fluorometer}, volume={217}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7201167}, DOI={10.1126/science.7201167}, number={4559}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Mayevsky, Avraham and Chance, Britton}, year={1982}, month=aug, pages={537–540} }

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