DOI: 10.1126/science.7112126. Fluorescence Microscopy: Reduced Photobleaching of Rhodamine and Fluorescein Protein Conjugates by <i>n</i> -Propyl Gallate

Fluorescence Microscopy: Reduced Photobleaching of Rhodamine and Fluorescein Protein Conjugates by n -Propyl Gallate

10.1126/science.7112126

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

n -Propyl gallate (0.1 to 0.25 molar, in glycerol) reduces by a factor of 10 the rate of fading of fluorescence of cell structures labeled with tetramethylrhodamine or fluorescein-conjugated antibodies. Hence, prolonged photographic exposure of immunofluorescently labeled cells in the fluorescence microscope yields images with increased sensitivity, making feasible multiple data collection, as with serial optical sectioning.

Bibliography

Giloh, H., & Sedat, J. W. (1982). Fluorescence Microscopy: Reduced Photobleaching of Rhodamine and Fluorescein Protein Conjugates by n -Propyl Gallate. Science, 217(4566), 1252â1255.

Authors 2

Haim Giloh (first)
John W. Sedat (additional)

References 22 Referenced 643

AGARD, D.A., PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS 264: 110 (1980). / PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (1980)
BURRIDGE, K, MICRO-INJECTION AND LOCALIZATION OF A 130K-PROTEIN IN LIVING FIBROBLASTS - RELATIONSHIP TO ACTIN AND FIBRONECTIN, CELL 19: 587 (1980). (10.1016/S0092-8674(80)80035-3) / CELL (1980)
ECHALIER, G, IN-VITRO CULTURE OF DROSOPHILA-MELANOGASTER EMBRYONIC CELLS, IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION 6: 162 (1970). / IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION (1970)
GILL, D, INHIBITION OF FADING IN FLUORESCENCE MICROSCOPY OF FIXED CELLS, EXPERIENTIA 35: 400 (1979). (10.1007/BF01964376) / EXPERIENTIA (1979)
HIJMANS W ANNALS OF THE NEW YORK ACADEMY OF SCIENCES 254 (1975). (10.1111/j.1749-6632.1975.tb29150.x)
JOHNSON, G.D., A SIMPLE METHOD OF REDUCING THE FADING OF IMMUNOFLUORESCENCE DURING MICROSCOPY, JOURNAL OF IMMUNOLOGICAL METHODS 43: 349 (1981). (10.1016/0022-1759(81)90183-6) / JOURNAL OF IMMUNOLOGICAL METHODS (1981)
JOHNSON, L.V., MONITORING OF RELATIVE MITOCHONDRIAL-MEMBRANE POTENTIAL IN LIVING CELLS BY FLUORESCENCE MICROSCOPY, JOURNAL OF CELL BIOLOGY 88: 526 (1981). (10.1083/jcb.88.3.526) / JOURNAL OF CELL BIOLOGY (1981)
Kawamura A. Fluorescent Antibody Techniques and Their Applications (1969).
KEITH, C.H., DIRECT VISUALIZATION OF FLUORESCEIN-LABELED MICROTUBULES INVITRO AND IN MICRO-INJECTED FIBROBLASTS, JOURNAL OF CELL BIOLOGY 88: 234 (1981). (10.1083/jcb.88.1.234) / JOURNAL OF CELL BIOLOGY (1981)
KREIS, T.E., INVIVO DISTRIBUTION AND TURNOVER OF FLUORESCENTLY LABELED ACTIN MICROINJECTED INTO HUMAN-FIBROBLASTS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 76: 3814 (1979). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1979)
Kuo, C.-H., Experimental Cell Research 142: 141 (1982). (10.1016/0014-4827(82)90418-9) / Experimental Cell Research (1982)
Langer H. Biochemistry and Physiology of Visual Pigments (1973). (10.1007/978-3-642-85769-0)
LIN, JJC, DISRUPTION OF THE INVIVO DISTRIBUTION OF THE INTERMEDIATE FILAMENTS IN FIBROBLASTS THROUGH THE MICRO-INJECTION OF A SPECIFIC MONOCLONAL-ANTIBODY, CELL 24: 185 (1981). (10.1016/0092-8674(81)90514-6) / CELL (1981)
ROBB, J.A., MAINTENANCE OF IMAGINAL DISCS OF DROSOPHILA MELANOGASTER IN CHEMICALLY DEFINED MEDIA, JOURNAL OF CELL BIOLOGY 41: 876 (1969). (10.1083/jcb.41.3.876) / JOURNAL OF CELL BIOLOGY (1969)
Sedat, J., Cold Spring Harbor Symposia on Quantitative Biology 42: 331 (1978). (10.1101/SQB.1978.042.01.035) / Cold Spring Harbor Symposia on Quantitative Biology (1978)
STACEY, D.W., EVIDENCE FOR AUTOPHAGY OF MICROINJECTED PROTEINS IN HELA-CELLS, JOURNAL OF CELL BIOLOGY 75: 807 (1977). (10.1083/jcb.75.3.807) / JOURNAL OF CELL BIOLOGY (1977)
STEWART, W.W., LUCIFER DYES - HIGHLY FLUORESCENT DYES FOR BIOLOGICAL TRACING, NATURE 292: 17 (1981). (10.1038/292017a0) / NATURE (1981)
TAYLOR, D.L., MOLECULAR CYTOCHEMISTRY - INCORPORATION OF FLUORESCENTLY LABELED ACTIN INTO LIVING CELLS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 75: 857 (1978). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1978)
VAUGHAN, W.M., OXYGEN QUENCHING OF PYRENEBUTYRIC ACID FLUORESCENCE IN WATER . A DYNAMIC PROBE OF MICROENVIRONMENT, BIOCHEMISTRY 9: 464 (1970). (10.1021/bi00805a003) / BIOCHEMISTRY (1970)
WANG, Y.L., JOURNAL OF CELL BIOLOGY 82: 672 (1979). / JOURNAL OF CELL BIOLOGY (1979)
WEBER, G, DETERMINATION OF THE ABSOLUTE QUANTUM YIELD OF FLUORESCENT SOLUTIONS, TRANSACTIONS OF THE FARADAY SOCIETY 53: 646 (1957). (10.1039/tf9575300646) / TRANSACTIONS OF THE FARADAY SOCIETY (1957)
10.1126/science.6159680

Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 2:30 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 5:56 p.m.)
Indexed	2 days, 2 hours ago (Sept. 4, 2025, 9:14 a.m.)
Issued	42 years, 11 months ago (Sept. 24, 1982)
Published	42 years, 11 months ago (Sept. 24, 1982)
Published Print	42 years, 11 months ago (Sept. 24, 1982)

Funders 0

None

BibTeX

@article{Giloh_1982, title={Fluorescence Microscopy: Reduced Photobleaching of Rhodamine and Fluorescein Protein Conjugates by n -Propyl Gallate}, volume={217}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7112126}, DOI={10.1126/science.7112126}, number={4566}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Giloh, Haim and Sedat, John W.}, year={1982}, month=sep, pages={1252–1255} }

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