DOI: 10.1126/science.7053567. Liposomes as Gene Carriers: Efficient Transformation of Mouse L Cells by Thymidine Kinase Gene

Liposomes as Gene Carriers: Efficient Transformation of Mouse L Cells by Thymidine Kinase Gene

10.1126/science.7053567

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Stable transformation of mouse L cells deficient in thymidine kinase was achieved by liposome-mediated transfer of a recombinant plasmid carrying the thymidine kinase gene. Ten percent of the recipient cells expressed thymidine kinase activity. The transformed phenotype (for example, 200 out of 10 6 cells) was stable under selective and nonselective conditions. The liposome technique is compared with other methods currently used for gene transfer.

Bibliography

Schaefer-Ridder, M., Wang, Y., & Hofschneider, P. H. (1982). Liposomes as Gene Carriers: Efficient Transformation of Mouse L Cells by Thymidine Kinase Gene. Science, 215(4529), 166â168.

Authors 3

Maria Schaefer-Ridder (first)
Yuan Wang (additional)
Peter Hans Hofschneider (additional)

References 18 Referenced 126

CAPECCHI, M.R., HIGH-EFFICIENCY TRANSFORMATION BY DIRECT MICRO-INJECTION OF DNA INTO CULTURED MAMMALIAN-CELLS, CELL 22: 479 (1980). (10.1016/0092-8674(80)90358-X) / CELL (1980)
COLBEREGARAPIN, F, CLONING OF THE ACTIVE THYMIDINE KINASE GENE OF HERPES-SIMPLEX VIRUS TYPE-1 IN ESCHERICHIA-COLI K-12, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 76: 3755 (1979). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1979)
DIACUMAKOS, E.G., METHOD CELL BIOL 7: 287 (1973). / METHOD CELL BIOL (1973)
FORGE, A, MORPHOLOGY OF EGG PHOSPHATIDYLCHOLINE-CHOLESTEROL SINGLE-BILAYER VESICLES, STUDIED BY FREEZE-ETCH ELECTRON-MICROSCOPY, JOURNAL OF MEMBRANE BIOLOGY 41: 249 (1978). (10.1007/BF01870432) / JOURNAL OF MEMBRANE BIOLOGY (1978)
FRALEY, R, INTRODUCTION OF LIPOSOME-ENCAPSULATED SV40 DNA INTO CELLS, JOURNAL OF BIOLOGICAL CHEMISTRY 255: 431 (1980). / JOURNAL OF BIOLOGICAL CHEMISTRY (1980)
10.1016/0042-6822(73)90341-3
GREGORIADIS, G, TAILORING LIPOSOME STRUCTURE, NATURE 283: 814 (1980). / NATURE (1980)
HAMER, D.H., SPLICING AND THE FORMATION OF STABLE RNA, CELL 18: 1299 (1979). (10.1016/0092-8674(79)90240-X) / CELL (1979)
HOEKSTRA, D, EFFECT OF FETAL CALF SERUM AND SERUM-PROTEIN FRACTIONS ON THE UPTAKE OF LIPOSOMAL PHOSPHATIDYLCHOLINE BY RAT HEPATOCYTES IN PRIMARY MONOLAYER-CULTURE, BIOCHIMICA ET BIOPHYSICA ACTA 551: 109 (1979). (10.1016/0005-2736(79)90357-2) / BIOCHIMICA ET BIOPHYSICA ACTA (1979)
KIRBY, C, EFFECT OF THE CHOLESTEROL CONTENT OF SMALL UNILAMELLAR LIPOSOMES ON THEIR STABILITY INVIVO AND INVITRO, BIOCHEMICAL JOURNAL 186: 591 (1980). (10.1042/bj1860591) / BIOCHEMICAL JOURNAL (1980)
Milman G. Banbury Conference Cold Spring Harbor Laboratory Cold Spring Harbor N.Y. 11 December (1980).
MULLIGAN, R.C., SYNTHESIS OF RABBIT BETA-GLOBIN IN CULTURED MONKEY KIDNEY CELLS FOLLOWING INFECTION WITH A SV40 BETA-GLOBIN RECOMBINANT GENOME, NATURE 277: 108 (1979). (10.1038/277108a0) / NATURE (1979)
PELLICER, A, TRANSFER AND STABLE INTEGRATION OF HSV THYMIDINE KINASE GENE INTO MOUSE CELLS, CELL 14: 133 (1978). (10.1016/0092-8674(78)90308-2) / CELL (1978)
RIGBY, PWJ, LABELING DEOXYRIBONUCLEIC-ACID TO HIGH SPECIFIC ACTIVITY INVITRO BY NICK TRANSLATION WITH DNA-POLYMERASE I, JOURNAL OF MOLECULAR BIOLOGY 113: 237 (1977). (10.1016/0022-2836(77)90052-3) / JOURNAL OF MOLECULAR BIOLOGY (1977)
STEIN, G.H., METHODS IN ENZYMOLOGY 58: 279 (1979). (10.1016/S0076-6879(79)58143-9) / METHODS IN ENZYMOLOGY (1979)
SZOKA, F, PROCEDURE FOR PREPARATION OF LIPOSOMES WITH LARGE INTERNAL AQUEOUS SPACE AND HIGH CAPTURE BY REVERSE-PHASE EVAPORATION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 75: 4194 (1978). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1978)
WIGLER, M, TRANSFER OF PURIFIED HERPES-VIRUS THYMIDINE KINASE GENE TO CULTURED MOUSE CELLS, CELL 11: 223 (1977). (10.1016/0092-8674(77)90333-6) / CELL (1977)
WONG, T.K., APPEARANCE OF BETA-LACTAMASE ACTIVITY IN ANIMAL-CELLS UPON LIPOSOME-MEDIATED GENE-TRANSFER, GENE 10: 87 (1980). (10.1016/0378-1119(80)90126-2) / GENE (1980)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 2:30 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 5:16 p.m.)
Indexed	3 months ago (May 22, 2025, 5:48 a.m.)
Issued	43 years, 7 months ago (Jan. 8, 1982)
Published	43 years, 7 months ago (Jan. 8, 1982)
Published Print	43 years, 7 months ago (Jan. 8, 1982)

Funders 0

None

BibTeX

@article{Schaefer_Ridder_1982, title={Liposomes as Gene Carriers: Efficient Transformation of Mouse L Cells by Thymidine Kinase Gene}, volume={215}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7053567}, DOI={10.1126/science.7053567}, number={4529}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Schaefer-Ridder, Maria and Wang, Yuan and Hofschneider, Peter Hans}, year={1982}, month=jan, pages={166–168} }

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