DOI: 10.1126/science.7046050. Lethal Disruption of the Yeast Actin Gene by Integrative DNA Transformation

Lethal Disruption of the Yeast Actin Gene by Integrative DNA Transformation

10.1126/science.7046050

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A mutant allele of the chromosomal locus corresponding to the cloned actin gene of the yeast Saccharomyces cerevisiae has been constructed by DNA transformation with a hybrid plasmid which integrates into, and thereby disrupts, the protein-encoding sequences of the gene. In a diploid strain of yeast, disruption of the actin gene on one chromosome results in a mutation that segregates as a recessive lethal tightly linked to a selectable genetic marker on the integrated plasmid. The actin gene, therefore, must encode an essential function for yeast cell growth.

Bibliography

Shortle, D., Haber, J. E., & Botstein, D. (1982). Lethal Disruption of the Yeast Actin Gene by Integrative DNA Transformation. Science, 217(4557), 371â373.

Authors 3

David Shortle (first)
James E. Haber (additional)
David Botstein (additional)

References 15 Referenced 189

BOTSTEIN, D, STERILE HOST YEASTS (SHY) - EUKARYOTIC SYSTEM OF BIOLOGICAL CONTAINMENT FOR RECOMBINANT DNA EXPERIMENTS, GENE 8: 17 (1979). (10.1016/0378-1119(79)90004-0) / GENE (1979)
DAVIS R.W. MANUAL GENETIC ENG A (1980).
GALLWITZ, D, MOLECULAR-CLONING OF THE ACTIN GENE FROM YEAST SACCHAROMYCES CEREVISIAE, NUCLEIC ACIDS RESEARCH 8: 1043 (1980). (10.1093/nar/8.5.1043) / NUCLEIC ACIDS RESEARCH (1980)
Gallwitz, D., Proceedings of the National Academy of Sciences of the United States of America 77: 2546 (1980). / Proceedings of the National Academy of Sciences of the United States of America (1980)
GOLDMAN R CELL MOTILITY 3 (1976).
HINNEN, A, TRANSFORMATION OF YEAST, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 75: 1929 (1978). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1978)
KLECKNER, N, GENETIC ENGINEERING INVIVO USING TRANSLOCATABLE DRUG-RESISTANCE ELEMENTS - NEW METHODS IN BACTERIAL GENETICS, JOURNAL OF MOLECULAR BIOLOGY 116: 125 (1977). (10.1016/0022-2836(77)90123-1) / JOURNAL OF MOLECULAR BIOLOGY (1977)
KORN, E.D., BIOCHEMISTRY OF ACTOMYOSIN-DEPENDENT CELL MOTILITY - REVIEW, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 75: 588 (1978). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1978)
MORTIMER, R.K., YEASTS 1: 385 (1969). / YEASTS (1969)
Ng, R., Proceedings of the National Academy of Sciences of the United States of America 77: 3912 (1980). / Proceedings of the National Academy of Sciences of the United States of America (1980)
Orr-Weaver, T. L., Proceedings of the National Academy of Sciences of the United States of America 78: 6354 (1981). / Proceedings of the National Academy of Sciences of the United States of America (1981)
SHORTLE D unpublished data.
10.1016/S0022-2836(75)80083-0
THOMAS J unpublished data.
WATER, R.D., IDENTIFICATION OF AN ACTIN-LIKE PROTEIN AND OF ITS MESSENGER RIBONUCLEIC-ACID IN SACCHAROMYCES-CEREVISIAE, JOURNAL OF BACTERIOLOGY 144: 1143 (1980). (10.1128/jb.144.3.1143-1151.1980) / JOURNAL OF BACTERIOLOGY (1980)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 2:30 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 6:02 p.m.)
Indexed	1 year, 4 months ago (April 7, 2024, 1:52 a.m.)
Issued	43 years ago (July 23, 1982)
Published	43 years ago (July 23, 1982)
Published Print	43 years ago (July 23, 1982)

Funders 0

None

BibTeX

@article{Shortle_1982, title={Lethal Disruption of the Yeast Actin Gene by Integrative DNA Transformation}, volume={217}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7046050}, DOI={10.1126/science.7046050}, number={4557}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Shortle, David and Haber, James E. and Botstein, David}, year={1982}, month=jul, pages={371–373} }

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