DOI: 10.1126/science.7043735. Ribosomal Crystalline Arrays of Large Subunits from <i>Escherichia coli</i>

Ribosomal Crystalline Arrays of Large Subunits from Escherichia coli

10.1126/science.7043735

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Crystalline sheets of the 50 S ribosomal subunits of Escherichia coli have been formed in vitro. Electron micrographs of these arrays diffract to 35-angstrom resolution. The lattice parameters of the crystals are a = 330 ± 20 angstroms, b = 330 ± 30 angstroms, and α = 123° ± 5°, and the space group is most likely p21. These arrays of ribosomal subunits are sufficiently ordered to resolve such known features of the large ribosomal subunit as the L7/L12 stalk and the central protuberance.

Bibliography

Clark, M. W., Leonard, K., & Lake, J. A. (1982). Ribosomal Crystalline Arrays of Large Subunits from Escherichia coli. Science, 216(4549), 999â1001.

Authors 3

Michael W. Clark (first)
Kevin Leonard (additional)
James A. Lake (additional)

References 11 Referenced 19

CLARK, M.W., HELICAL ARRAYS OF ESCHERICHIA-COLI SMALL RIBOSOMAL-SUBUNITS PRODUCED INVITRO, JOURNAL OF MOLECULAR BIOLOGY 135: 507 (1979). (10.1016/0022-2836(79)90450-9) / JOURNAL OF MOLECULAR BIOLOGY (1979)
10.1038/217130a0
FULLER, S.D., STRUCTURE OF CYTOCHROME-C OXIDASE IN DEOXYCHOLATE-DERIVED 2-DIMENSIONAL CRYSTALS, JOURNAL OF MOLECULAR BIOLOGY 134: 305 (1979). (10.1016/0022-2836(79)90037-8) / JOURNAL OF MOLECULAR BIOLOGY (1979)
GESTELAND, R.F., UNFOLDING OF ESCHERICHIA COLI RIBOSOMES BY REMOVAL OF MAGNESIUM, JOURNAL OF MOLECULAR BIOLOGY 18: 356 (1966). (10.1016/S0022-2836(66)80253-X) / JOURNAL OF MOLECULAR BIOLOGY (1966)
LAKE, J.A., RIBOSOMAL-PROTEINS L1, L17 AND L27 FROM ESCHERICHIA-COLI LOCALIZED AT SINGLE SITES ON THE LARGE SUBUNIT BY IMMUNE ELECTRON-MICROSCOPY, JOURNAL OF MOLECULAR BIOLOGY 153: 979 (1981). (10.1016/0022-2836(81)90462-9) / JOURNAL OF MOLECULAR BIOLOGY (1981)
LAKE, J.A., 3-DIMENSIONAL STRUCTURE OF CHROMATOID BODY HELIX OF ENTAMOEBA-INVADENS, JOURNAL OF MOLECULAR BIOLOGY 66: 271 (1972). (10.1016/0022-2836(72)90478-0) / JOURNAL OF MOLECULAR BIOLOGY (1972)
LAKE, J.A., RIBOSOMES STRUCTURE: 207 (1979). / RIBOSOMES STRUCTURE (1979)
STRYCHARZ, W.A., RIBOSOMAL PROTEINS-L7-L12 LOCALIZED AT A SINGLE REGION OF THE LARGE SUBUNIT BY IMMUNE ELECTRON-MICROSCOPY, JOURNAL OF MOLECULAR BIOLOGY 126: 123 (1978). (10.1016/0022-2836(78)90355-8) / JOURNAL OF MOLECULAR BIOLOGY (1978)
UNWIN, PNT, 3-DIMENSIONAL MODEL OF MEMBRANE-BOUND RIBOSOMES OBTAINED BY ELECTRON-MICROSCOPY, NATURE 269: 118 (1977). (10.1038/269118a0) / NATURE (1977)
WINKELMANN, D, RIBOSOMES STRUCTURE: 255 (1979). / RIBOSOMES STRUCTURE (1979)
YONATH, A.E., CRYSTALLIZATION OF THE LARGE RIBOSOMAL-SUBUNITS FROM BACILLUS-STEAROTHERMOPHILUS, BIOCHEMISTRY INTERNATIONAL 1: 428 (1980). / BIOCHEMISTRY INTERNATIONAL (1980)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 2:30 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 6:32 p.m.)
Indexed	11 months, 1 week ago (Sept. 15, 2024, 6:15 p.m.)
Issued	43 years, 2 months ago (May 28, 1982)
Published	43 years, 2 months ago (May 28, 1982)
Published Print	43 years, 2 months ago (May 28, 1982)

Funders 0

None

BibTeX

@article{Clark_1982, title={Ribosomal Crystalline Arrays of Large Subunits from Escherichia coli}, volume={216}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7043735}, DOI={10.1126/science.7043735}, number={4549}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Clark, Michael W. and Leonard, Kevin and Lake, James A.}, year={1982}, month=may, pages={999–1001} }

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