DOI: 10.1126/science.7036343. Cytoplasmic Inclusion Bodies in <i>Escherichia coli</i> Producing Biosynthetic Human Insulin Proteins

Cytoplasmic Inclusion Bodies in Escherichia coli Producing Biosynthetic Human Insulin Proteins

10.1126/science.7036343

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Escherichia coli that has been genetically manipulated by recombinant DNA technology to synthesize human insulin polypeptides (A chain, B chain, or proinsulin) contains prominent cytoplasmic inclusion bodies. The amount of inclusion product within the cells corresponds to the quantity of chimeric protein formed by the bacteria. At peak production, the inclusion bodies may occupy as much as 20 percent of the Escherichia coli cellular volume.

Bibliography

Williams, D. C., Van Frank, R. M., Muth, W. L., & Burnett, J. P. (1982). Cytoplasmic Inclusion Bodies in Escherichia coli Producing Biosynthetic Human Insulin Proteins. Science, 215(4533), 687â689.

Authors 4

Daniel C. Williams (first)
Richard M. Van Frank (additional)
William L. Muth (additional)
J. Paul Burnett (additional)

References 12 Referenced 258

COSTERTON, J.W., ROLE OF ELECTRON-MICROSCOPY IN THE ELUCIDATION OF BACTERIAL STRUCTURE AND FUNCTION, ANNUAL REVIEW OF MICROBIOLOGY 33: 459 (1979). (10.1146/annurev.mi.33.100179.002331) / ANNUAL REVIEW OF MICROBIOLOGY (1979)
CREA, R, CHEMICAL SYNTHESIS OF GENES FOR HUMAN INSULIN, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 75: 5765 (1978). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1978)
LAISHLEY, E.J., ISOLATION AND MORPHOLOGY OF NATIVE INTRACELLULAR POLYGLUCOSE GRANULES FROM CLOSTRIDIUM-PASTEURIANUM, CANADIAN JOURNAL OF MICROBIOLOGY 19: 991 (1973). (10.1139/m73-157) / CANADIAN JOURNAL OF MICROBIOLOGY (1973)
10.1016/S0021-9258(19)41496-8
PAUL D.C. unpublished data.
PFISTER, R.M., ELECTRON MICROSCOPY OF POLYRIBOSOMES WITHIN BACILLUS CEREUS, JOURNAL OF BACTERIOLOGY 88: 1119 (1964). (10.1128/jb.88.4.1119-1129.1964) / JOURNAL OF BACTERIOLOGY (1964)
PROUTY, W.F., DEGRADATION OF ABNORMAL PROTEINS IN ESCHERICHIA-COLI FORMATION OF PROTEIN INCLUSIONS IN CELLS EXPOSED TO AMINO-ACID ANALOGS, JOURNAL OF BIOLOGICAL CHEMISTRY 250: 1112 (1975). (10.1016/S0021-9258(19)41897-8) / JOURNAL OF BIOLOGICAL CHEMISTRY (1975)
RABINOVITZ, M, AMINO ACID ANTAGONIST DEATH IN ESCHERICHIA COLI, JOURNAL OF BACTERIOLOGY 99: 336 (1969). (10.1128/jb.99.1.336-338.1969) / JOURNAL OF BACTERIOLOGY (1969)
SCHACHTELE, F.C., J MOL BIOL 33: 861 (1968). (10.1016/0022-2836(68)90324-0) / J MOL BIOL (1968)
SHIVELY, J.M., INCLUSION-BODIES OF PROKARYOTES, ANNUAL REVIEW OF MICROBIOLOGY 28: 167 (1974). (10.1146/annurev.mi.28.100174.001123) / ANNUAL REVIEW OF MICROBIOLOGY (1974)
WEIBEL, E.R., PRINCIPLES TECHNIQUE 3: 237 (1973). / PRINCIPLES TECHNIQUE (1973)
WILLIAMS, D.C., J CELL BIOL 92: A217 (1981). / J CELL BIOL (1981)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 2:30 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 5:10 p.m.)
Indexed	3 weeks, 4 days ago (Aug. 2, 2025, 12:23 a.m.)
Issued	43 years, 6 months ago (Feb. 5, 1982)
Published	43 years, 6 months ago (Feb. 5, 1982)
Published Print	43 years, 6 months ago (Feb. 5, 1982)

Funders 0

None

BibTeX

@article{Williams_1982, title={Cytoplasmic Inclusion Bodies in Escherichia coli Producing Biosynthetic Human Insulin Proteins}, volume={215}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.7036343}, DOI={10.1126/science.7036343}, number={4533}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Williams, Daniel C. and Van Frank, Richard M. and Muth, William L. and Burnett, J. Paul}, year={1982}, month=feb, pages={687–689} }

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