DOI: 10.1126/science.6857285. Synthesis and Secretion of the Plasmid-Coded Heat-Labile Enterotoxin of <i>Escherichia coli</i> in <i>Vibrio cholerae</i>

Synthesis and Secretion of the Plasmid-Coded Heat-Labile Enterotoxin of Escherichia coli in Vibrio cholerae

10.1126/science.6857285

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Both cholera toxin and heat-labile enterotoxin were made and secreted into culture supernatants by Vibrio cholerae containing the enterotoxin plasmid pCG86. Several regulatory mutations in V. cholerae that increased or decreased the synthesis of cholera toxin did not affect production of heat-labile enterotoxin. In contrast, a mutation in V. cholerae that interfered with the secretion of cholera toxin also decreased the secretion of heat-labile enterotoxin, indicating that they are processed by a common secretory pathway. Vibrio cholerae should be useful as a model system for analyzing the secretion of true extracellular proteins by Gram-negative bacteria.

Bibliography

Neill, R. J., Ivins, B. E., & Holmes, R. K. (1983). Synthesis and Secretion of the Plasmid-Coded Heat-Labile Enterotoxin of Escherichia coli in Vibrio cholerae. Science, 221(4607), 289â291.

Authors 3

Roger J. Neill (first)
Bruce E. Ivins (additional)
Randall K. Holmes (additional)

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Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 2:37 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 5:31 p.m.)
Indexed	1 year, 3 months ago (May 29, 2024, 7:08 p.m.)
Issued	42 years, 1 month ago (July 15, 1983)
Published	42 years, 1 month ago (July 15, 1983)
Published Print	42 years, 1 month ago (July 15, 1983)

Funders 0

None

BibTeX

@article{Neill_1983, title={Synthesis and Secretion of the Plasmid-Coded Heat-Labile Enterotoxin of Escherichia coli in Vibrio cholerae}, volume={221}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.6857285}, DOI={10.1126/science.6857285}, number={4607}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Neill, Roger J. and Ivins, Bruce E. and Holmes, Randall K.}, year={1983}, month=jul, pages={289–291} }

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