DOI: 10.1126/science.6836293. Electron Microscope Tomography: Transcription in Three Dimensions

Electron Microscope Tomography: Transcription in Three Dimensions

10.1126/science.6836293

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Three-dimensional reconstruction of an asymmetric biological ultrastructure has been achieved by tomographic analysis of electron micrographs of sections tilted on a goniometer specimen stage. Aligned micrographs could be displayed as red-green three-dimensional movies. The techniques have been applied to portions of in situ transcription units of a Balbiani ring in the polytene chromosomes of the midge Chironomus tentans . Current data suggest a DNA compaction of about 8 to 1 in a transcription unit. Nascent ribonucleoprotein granules display an imperfect sixfold helical arrangement around the chromatin axis.

Bibliography

Olins, D. E., Olins, A. L., Levy, H. A., Durfee, R. C., Margle, S. M., Tinnel, E. P., & Dover, S. D. (1983). Electron Microscope Tomography: Transcription in Three Dimensions. Science, 220(4596), 498â500.

Authors 7

Donald E. Olins (first)
Ada L. Olins (additional)
Henri A. Levy (additional)
Richard C. Durfee (additional)
Stephen M. Margle (additional)
Ed P. Tinnel (additional)
S. David Dover (additional)

References 17 Referenced 85

ANDERSSON, K, THE INSITU STRUCTURE OF THE ACTIVE 75-S RNA GENES IN BALBIANI RINGS OF CHIRONOMUS-TENTANS, EXPERIMENTAL CELL RESEARCH 130: 313 (1980). (10.1016/0014-4827(80)90008-7) / EXPERIMENTAL CELL RESEARCH (1980)
BEERMANN, W, RESULTS AND PROBLEMS IN CELL DIFFERENTIATION 4: 1 (1972). (10.1007/978-3-540-37164-9_1) / RESULTS AND PROBLEMS IN CELL DIFFERENTIATION (1972)
10.1126/science.215.4533.619
CROWTHER, R.A., RECONSTRUCTION OF 3 DIMENSIONAL STRUCTURE FROM PROJECTIONS AND ITS APPLICATION TO ELECTRON MICROSCOPY, PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES A-MATHEMATICAL AND PHYSICAL SCIENCES 317: 319 (1970). / PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES A-MATHEMATICAL AND PHYSICAL SCIENCES (1970)
DANEHOLT B INSECT ULTRASTRUCTUR (1982).
DOVER, S.D., 3-DIMENSIONAL RECONSTRUCTION FROM IMAGES OF TILTED SPECIMENS - THE PARAMYOSIN FILAMENT, JOURNAL OF MICROSCOPY-OXFORD 122: 23 (1981). (10.1111/j.1365-2818.1981.tb01239.x) / JOURNAL OF MICROSCOPY-OXFORD (1981)
EDSTROM, J.E., CELL NUCLEUS 2: 293 (1974). / CELL NUCLEUS (1974)
HERMAN G.T. IMAGE RECONSTRUCTION (1980).
HOPPE, W, TOPICS IN CURRENT PHYSICS 13: 127 (1980). (10.1007/978-3-642-81381-8_4) / TOPICS IN CURRENT PHYSICS (1980)
LAMB, M.M., CHARACTERIZATION OF ACTIVE TRANSCRIPTION UNITS IN BALBIANI RINGS OF CHIRONOMUS TENTANS, CELL 17: 835 (1979). (10.1016/0092-8674(79)90324-6) / CELL (1979)
LEVY H.A. unpublished data.
LEZZI, M, HEAT-SHOCK PHENOMENA IN CHIRONOMUS-TENTANS .1. INVIVO EFFECTS OF HEAT, OVER-HEAT, AND QUENCHING ON SALIVARY CHROMOSOME PUFFING, CHROMOSOMA 83: 327 (1981). (10.1007/BF00327356) / CHROMOSOMA (1981)
MAHR, R, ACTIVATION OF BALBIANI RING GENES IN CHIRONOMUS-TENTANS AFTER A PILOCARPINE-INDUCED DEPLETION OF THE SECRETORY PRODUCTS FROM THE SALIVARY-GLAND LUMEN, DEVELOPMENTAL BIOLOGY 80: 409 (1980). (10.1016/0012-1606(80)90415-7) / DEVELOPMENTAL BIOLOGY (1980)
MELLAMA, J.E., TOPICS CURRENT PHYSI 13: 89 (1980). (10.1007/978-3-642-81381-8_3) / TOPICS CURRENT PHYSI (1980)
Meyer, B., Developmental Biology 98: 265 (1983). (10.1016/0012-1606(83)90357-3) / Developmental Biology (1983)
OLINS, A.L., STEREO-ELECTRON MICROSCOPY OF NUCLEOLI, BALBIANI RINGS AND ENDOPLASMIC-RETICULUM IN CHIRONOMUS SALIVARY-GLAND CELLS, EUROPEAN JOURNAL OF CELL BIOLOGY 22: 714 (1980). / EUROPEAN JOURNAL OF CELL BIOLOGY (1980)
OLINS, A.L., ULTRASTRUCTURAL STUDIES OF CHIRONOMUS SALIVARY-GLAND CELLS IN DIFFERENT STATES OF BALBIANI RING ACTIVITY, EUROPEAN JOURNAL OF CELL BIOLOGY 27: 161 (1982). / EUROPEAN JOURNAL OF CELL BIOLOGY (1982)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 2:37 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 5:23 p.m.)
Indexed	3 weeks, 1 day ago (Aug. 6, 2025, 8:34 a.m.)
Issued	42 years, 4 months ago (April 29, 1983)
Published	42 years, 4 months ago (April 29, 1983)
Published Print	42 years, 4 months ago (April 29, 1983)

Funders 0

None

BibTeX

@article{Olins_1983, title={Electron Microscope Tomography: Transcription in Three Dimensions}, volume={220}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.6836293}, DOI={10.1126/science.6836293}, number={4596}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Olins, Donald E. and Olins, Ada L. and Levy, Henri A. and Durfee, Richard C. and Margle, Stephen M. and Tinnel, Ed P. and Dover, S. David}, year={1983}, month=apr, pages={498–500} }

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