DOI: 10.1126/science.6823553. Attogram Detection Limit for Aqueous Dye Samples by Laser-Induced Fluorescence

Attogram Detection Limit for Aqueous Dye Samples by Laser-Induced Fluorescence

10.1126/science.6823553

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A modified flow cytometer has been used to detect attogram quantities of aqueous rhodamine 6G by laser-induced fluorescence analysis. A detection limit of 28 attograms (35,000 molecules) was obtained, nearly two orders of magnitude better than earlier measurements. The detection limit in concentration units was 1.4 × 10 -13 mole per liter. During the 1-second measurement period, the total volume sampled was 0.42 microliter. On average, only half a rhodamine 6G molecule was present in the 6-picoliter probed volume.

Authors 4

Norman J. Dovichi (first)
John C. Martin (additional)
James H. Jett (additional)
Richard A. Keller (additional)

References 11 Referenced 105

10.1126/science.867038
DOVICHI N.J. unpublished data.
FOLESTAD, S, LASER-INDUCED FLUORESCENCE DETECTION FOR CONVENTIONAL AND MICROCOLUMN LIQUID-CHROMATOGRAPHY, ANALYTICAL CHEMISTRY 54: 925 (1982). (10.1021/ac00243a019) / ANALYTICAL CHEMISTRY (1982)
HERSHBERGER, L.W., SUB-MICROLITER FLOW-THROUGH CUVETTE FOR FLUORESCENCE MONITORING OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC EFFLUENTS, ANALYTICAL CHEMISTRY 51: 1444 (1979). (10.1021/ac50045a021) / ANALYTICAL CHEMISTRY (1979)
HIRSCHFELD, T, QUANTUM EFFICIENCY INDEPENDENCE OF TIME INTEGRATED EMISSION FROM A FLUORESCENT MOLECULE, APPLIED OPTICS 15: 3135 (1976). (10.1364/AO.15.003135) / APPLIED OPTICS (1976)
KELLY, T.A., FLUOROMETER FOR FLOW-INJECTION ANALYSIS WITH APPLICATION TO OXIDASE ENZYME DEPENDENT REACTIONS, ANALYTICAL CHEMISTRY 53: 2110 (1981). (10.1021/ac00236a038) / ANALYTICAL CHEMISTRY (1981)
MELAMED M.R. FLOW CYTOMETRY SORTI (1979).
REIFFEN, B, AN OPTIMUM DEMODULATOR FOR POISSON PROCESSES - PHOTON SOURCE DETECTORS, PROCEEDINGS OF THE IEEE 51: 1316 (1963). (10.1109/PROC.1963.2562) / PROCEEDINGS OF THE IEEE (1963)
ROBINSON, T.J., ERYTHROCYTES - NEW CELL TYPE FOR THE EVALUATION OF INSULIN RECEPTOR DEFECTS IN DIABETIC HUMANS, SCIENCE 205: 200 (1979). (10.1126/science.451590) / SCIENCE (1979)
SMITH, D.S., MODERN FLUORESCENCE 3: 143 (1981). / MODERN FLUORESCENCE (1981)
YEUNG, E.S., LASER FLUOROMETRIC DETECTION IN LIQUID-CHROMATOGRAPHY, ANALYTICAL CHEMISTRY 52: A1465 (1980). (10.1021/ac50063a804) / ANALYTICAL CHEMISTRY (1980)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 2:37 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 5:43 p.m.)
Indexed	2 months ago (June 28, 2025, 8:23 a.m.)
Issued	42 years, 6 months ago (Feb. 18, 1983)
Published	42 years, 6 months ago (Feb. 18, 1983)
Published Print	42 years, 6 months ago (Feb. 18, 1983)

Funders 0

None

BibTeX

@article{Dovichi_1983, title={Attogram Detection Limit for Aqueous Dye Samples by Laser-Induced Fluorescence}, volume={219}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.6823553}, DOI={10.1126/science.6823553}, number={4586}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Dovichi, Norman J. and Martin, John C. and Jett, James H. and Keller, Richard A.}, year={1983}, month=feb, pages={845–847} }

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