DOI: 10.1126/science.6729453. Enzymatic Catalysis in Organic Media at 100°C

Enzymatic Catalysis in Organic Media at 100°C

10.1126/science.6729453

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Porcine pancreatic lipase catalyzes the transesterification reaction between tributyrin and various primary and secondary alcohols in a 99 percent organic medium. Upon further dehydration, the enzyme becomes extremely thermostable. Not only can the dry lipase withstand heating at 100°C for many hours, but it exhibits a high catalytic activity at that temperature. Reduction in water content also alters the substrate specificity of the lipase: in contrast to its wet counterpart, the dry enzyme does not react with bulky tertiary alcohols.

Bibliography

Zaks, A., & Klibanov, A. M. (1984). Enzymatic Catalysis in Organic Media at 100Â°C. Science, 224(4654), 1249â1251.

Authors 2

Aleksey Zaks (first)
Alexander M. Klibanov (additional)

References 22 Referenced 935

ANTONINI, E, ENZYME AND MICROBIAL TECHNOLOGY 3: 291 (1981). (10.1016/0141-0229(81)90002-8) / ENZYME AND MICROBIAL TECHNOLOGY (1981)
Brockerhoff H. Lypolytic Enzymes (1974). (10.1021/ba-1974-0136.ch005)
Cambou, B., Journal of the American Chemical Society 106: 2687 (1984). (10.1021/ja00321a033) / Journal of the American Chemical Society (1984)
Cantor C. R. BIOPHYSICAL CHEMISTRY (1980).
CARERI, G, CORRELATION OF IR-SPECTROSCOPIC, HEAT-CAPACITY, DIAMAGNETIC SUSCEPTIBILITY AND ENZYMATIC MEASUREMENTS ON LYSOZYME POWDER, NATURE 284: 572 (1980). (10.1038/284572a0) / NATURE (1980)
Desnuelle, P., The Enzymes 7: 575 (1972). (10.1016/S1874-6047(08)60463-1) / The Enzymes (1972)
Feeney, R. E., Chemical Deterioration of Proteins: 1 (1980). / Chemical Deterioration of Proteins (1980)
KAUZMANN, W, SOME FACTORS IN THE INTERPRETATION OF PROTEIN DENATURATION, ADVANCES IN PROTEIN CHEMISTRY 14: 1 (1959). (10.1016/S0065-3233(08)60608-7) / ADVANCES IN PROTEIN CHEMISTRY (1959)
KLIBANOV, A.M., STABILIZATION OF ENZYMES AGAINST THERMAL INACTIVATION, ADVANCES IN APPLIED MICROBIOLOGY 29: 1 (1983). (10.1016/S0065-2164(08)70352-6) / ADVANCES IN APPLIED MICROBIOLOGY (1983)
Laitinen H. A. Chemical Analysis 2: 361 (1975).
Lapanje S. Physico-chemical Aspects of Protein Denaturation (1978).
LILLY, M.D., 2-LIQUID-PHASE BIOCATALYTIC REACTIONS, JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 32: 162 (1982). (10.1002/jctb.5030320120) / JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY (1982)
MACRAE, A. R., JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY 60: 291 (1983). (10.1007/BF02543502) / JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY (1983)
10.1126/science.6753152
10.1126/science.223.4632.154
10.1126/science.6608147
POOLE, P.L., HYDRATION-INDUCED CONFORMATIONAL AND FLEXIBILITY CHANGES IN LYSOZYME AT LOW WATER-CONTENT, INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 5: 308 (1983). (10.1016/0141-8130(83)90047-8) / INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (1983)
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Schulz G. E. Principles of Protein Structure (1979). (10.1007/978-1-4612-6137-7)
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Tanford C. Physical Chemistry of Macromolecules (1961). (10.1149/1.2425390)
10.1126/science.6572017

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 3:41 p.m.)
Deposited	7 months, 2 weeks ago (Jan. 11, 2025, 2:27 a.m.)
Indexed	1 week ago (Aug. 24, 2025, 7:08 p.m.)
Issued	41 years, 2 months ago (June 15, 1984)
Published	41 years, 2 months ago (June 15, 1984)
Published Print	41 years, 2 months ago (June 15, 1984)

Funders 0

None

BibTeX

@article{Zaks_1984, title={Enzymatic Catalysis in Organic Media at 100°C}, volume={224}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.6729453}, DOI={10.1126/science.6729453}, number={4654}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Zaks, Aleksey and Klibanov, Alexander M.}, year={1984}, month=jun, pages={1249–1251} }

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