DOI: 10.1126/science.6691142. Heterogeneity of Normal Human Diploid Fibroblasts: Isolation and Characterization of One Phenotype

Heterogeneity of Normal Human Diploid Fibroblasts: Isolation and Characterization of One Phenotype

10.1126/science.6691142

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Cultures of human diploid fibroblasts contain cells that respond to exposure to the first component of complement (C1) by initiating DNA synthesis and growth. The plasma membranes of these cells have specific binding sites for the C1q subcomponent of C1. A fluorescence-activated cell sorter was used to isolate a subset of cells with a high affinity for C1q, and the growth and synthesis activities of these high-affinity cells were studied after numerous replications in vitro. These cells synthesize DNA and grow faster than the parent cultures and low-affinity cells, and they produce two to three times as much protein. About 40 percent of their total protein synthesis activity is directed to collagen production, unusually high proportions of collagen types III and V being produced. These properties and the high affinity of the cells for C1q are retained for at least six cell transfers. This phenotype has the properties expected of fibroblasts in healing wounds and inflamed tissues.

Bibliography

Bordin, S., Page, R. C., & Narayanan, A. S. (1984). Heterogeneity of Normal Human Diploid Fibroblasts: Isolation and Characterization of One Phenotype. Science, 223(4632), 171â173.

Authors 3

Sandra Bordin (first)
R. C. Page (additional)
A. S. Narayanan (additional)

References 27 Referenced 141

BORDIN, S, J IMMUNOL 130: 1871 (1982). / J IMMUNOL (1982)
BOTSTEIN, G.R., FIBROBLAST SELECTION IN SCLERODERMA - AN ALTERNATIVE MODEL OF FIBROSIS, ARTHRITIS AND RHEUMATISM 25: 189 (1982). (10.1002/art.1780250212) / ARTHRITIS AND RHEUMATISM (1982)
BRINCKERHOFF, C.E., COLLAGENASE PRODUCTION BY CLONED POPULATIONS OF RABBIT SYNOVIAL FIBROBLASTS, COLLAGEN AND RELATED RESEARCH 1: 433 (1981). (10.1016/S0174-173X(81)80027-1) / COLLAGEN AND RELATED RESEARCH (1981)
CARPENTER, G, EPIDERMAL GROWTH-FACTOR, ANNUAL REVIEW OF BIOCHEMISTRY 48: 193 (1979). (10.1146/annurev.bi.48.070179.001205) / ANNUAL REVIEW OF BIOCHEMISTRY (1979)
DIEGELMANN, R.F., EFFECT OF MACROPHAGES ON FIBROBLAST DNA-SYNTHESIS AND PROLIFERATION, PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE 169: 445 (1982). (10.3181/00379727-169-41373) / PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE (1982)
DIEGELMANN, R.F., GROWTH KINETICS AND COLLAGEN-SYNTHESIS OF NORMAL SKIN, NORMAL SCAR AND KELOID FIBROBLASTS INVITRO, JOURNAL OF CELLULAR PHYSIOLOGY 98: 341 (1979). (10.1002/jcp.1040980210) / JOURNAL OF CELLULAR PHYSIOLOGY (1979)
GLENN, K, PLATELET-DERIVED GROWTH-FACTOR .3. IDENTIFICATION OF A PLATELET-DERIVED GROWTH-FACTOR RECEPTOR BY AFFINITY LABELING, JOURNAL OF BIOLOGICAL CHEMISTRY 257: 5172 (1982). (10.1016/S0021-9258(18)34651-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1982)
GORDEN, P, EPIDERMAL GROWTH-FACTOR - MORPHOLOGICAL DEMONSTRATION OF BINDING, INTERNALIZATION, AND LYSOSOMAL ASSOCIATION IN HUMAN FIBROBLASTS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 75: 5025 (1978). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1978)
HASSELL, T.M., DIPHENYLHYDANTOIN (DILANTIN) GINGIVAL HYPERPLASIA - DRUG-INDUCED ABNORMALITY OF CONNECTIVE-TISSUE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 73: 2909 (1976). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1976)
HOLLENBERG, M.D., EPIDERMAL GROWTH-FACTOR - RECEPTORS IN HUMAN FIBROBLASTS AND MODULATION OF ACTION BY CHOLERA TOXIN, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 70: 2964 (1973). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1973)
HOLLEY, R.W., CONTROL OF GROWTH OF MAMMALIAN-CELLS IN CELL-CULTURE, NATURE 258: 487 (1975). (10.1038/258487a0) / NATURE (1975)
HUEY, J, EFFECT OF EPIDERMAL GROWTH-FACTOR ON THE SYNTHETIC ACTIVITY OF HUMAN-FIBROBLASTS, BIOCHIMICA ET BIOPHYSICA ACTA 632: 227 (1980). (10.1016/0304-4165(80)90080-X) / BIOCHIMICA ET BIOPHYSICA ACTA (1980)
JOHNSON, R.L., LYMPHOKINE STIMULATION OF COLLAGEN ACCUMULATION, JOURNAL OF CLINICAL INVESTIGATION 58: 240 (1976). (10.1172/JCI108455) / JOURNAL OF CLINICAL INVESTIGATION (1976)
KO, S.D., FIBROBLAST HETEROGENEITY AND PROSTAGLANDIN REGULATION OF SUBPOPULATIONS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 74: 3429 (1977). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1977)
KORN, Y.H., JOURNAL OF CLINICAL INVESTIGATION 71: 1240 (1983). (10.1172/JCI110873) / JOURNAL OF CLINICAL INVESTIGATION (1983)
KOROTZER, T.I., COMPLEMENT-DEPENDENT INDUCTION OF DNA-SYNTHESIS AND PROLIFERATION OF HUMAN-DIPLOID FIBROBLASTS, JOURNAL OF CELLULAR PHYSIOLOGY 105: 503 (1980). (10.1002/jcp.1041050314) / JOURNAL OF CELLULAR PHYSIOLOGY (1980)
KOROTZER, T.I., REGULATION OF GROWTH OF HUMAN-DIPLOID FIBROBLASTS BY FACTORS ELABORATED BY ACTIVATED LYMPHOID-CELLS, JOURNAL OF CELLULAR PHYSIOLOGY 111: 247 (1982). (10.1002/jcp.1041110305) / JOURNAL OF CELLULAR PHYSIOLOGY (1982)
LEROY, E.C., CONNECTIVE-TISSUE SYNTHESIS BY SCLERODERMA SKIN FIBROBLASTS IN CELL-CULTURE, JOURNAL OF EXPERIMENTAL MEDICINE 135: 1351 (1972). (10.1084/jem.135.6.1351) / JOURNAL OF EXPERIMENTAL MEDICINE (1972)
MARTIN, G.M., CLONAL SELECTION, ATTENUATION AND DIFFERENTIATION IN AN IN-VITRO MODEL OF HYPERPLASIA, AMERICAN JOURNAL OF PATHOLOGY 74: 137 (1974). / AMERICAN JOURNAL OF PATHOLOGY (1974)
NARAYANAN, A.S., CONNECTIVE TISSUES OF THE PERIODONTIUM - A SUMMARY OF CURRENT WORK, COLLAGEN AND RELATED RESEARCH 3: 33 (1983). (10.1016/S0174-173X(83)80048-X) / COLLAGEN AND RELATED RESEARCH (1983)
NARAYANAN, A.S., J BIOL CHEM 251: 5414 (1976). (10.1016/S0021-9258(17)33177-0) / J BIOL CHEM (1976)
Narayanan, A. S., Journal of Biological Chemistry 258: 11694 (1983). (10.1016/S0021-9258(17)44284-0) / Journal of Biological Chemistry (1983)
NARAYANAN, A.S., COLLAGENS SYNTHESIZED INVITRO BY DIPLOID FIBROBLASTS OBTAINED FROM CHRONICALLY INFLAMED HUMAN CONNECTIVE-TISSUE, LABORATORY INVESTIGATION 39: 61 (1978). / LABORATORY INVESTIGATION (1978)
PARDEE, A.B., ANIMAL-CELL CYCLE, ANNUAL REVIEW OF BIOCHEMISTRY 47: 715 (1978). (10.1146/annurev.bi.47.070178.003435) / ANNUAL REVIEW OF BIOCHEMISTRY (1978)
PEITILA, K, ATHEROSCLEROSIS 37: 11 (1980). (10.1016/0021-9150(80)90089-1) / ATHEROSCLEROSIS (1980)
ROSS, R, PLATELET-DERIVED GROWTH-FACTOR, CELL 14: 203 (1978). (10.1016/0092-8674(78)90107-1) / CELL (1978)
10.1126/science.7350644

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 3:41 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 6:15 p.m.)
Indexed	2 days, 8 hours ago (Aug. 28, 2025, 8:42 a.m.)
Issued	41 years, 7 months ago (Jan. 13, 1984)
Published	41 years, 7 months ago (Jan. 13, 1984)
Published Print	41 years, 7 months ago (Jan. 13, 1984)

Funders 0

None

BibTeX

@article{Bordin_1984, title={Heterogeneity of Normal Human Diploid Fibroblasts: Isolation and Characterization of One Phenotype}, volume={223}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.6691142}, DOI={10.1126/science.6691142}, number={4632}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Bordin, Sandra and Page, R. C. and Narayanan, A. S.}, year={1984}, month=jan, pages={171–173} }

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