DOI: 10.1126/science.6635659. Human Endothelial Cells: Use of Heparin in Cloning and Long-Term Serial Cultivation

Human Endothelial Cells: Use of Heparin in Cloning and Long-Term Serial Cultivation

10.1126/science.6635659

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Endothelial cells from human blood vessels were cultured in vitro, with doubling times of 17 to 21 hours for 42 to 79 population doublings. Cloned human endothelial cell strains were established for the first time and had similar proliferative capacities. This vigorous cell growth was achieved by addition of heparin to culture medium containing reduced concentrations of endothelial cell growth factor. The routine cloning and long-term culture of human endothelial cells will facilitate studying the human endothelium in vitro.

Bibliography

Thornton, S. C., Mueller, S. N., & Levine, E. M. (1983). Human Endothelial Cells: Use of Heparin in Cloning and Long-Term Serial Cultivation. Science, 222(4624), 623â625.

Authors 3

Susan C. Thornton (first)
Stephen N. Mueller (additional)
Elliot M. Levine (additional)

References 31 Referenced 568

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FRY, G.L., CIRCULATION 86: 177 (1982). / CIRCULATION (1982)
GAMSE, G, METABOLISM OF SULFATED GLYCOSAMINOGLYCANS IN CULTURED ENDOTHELIAL CELLS AND SMOOTH-MUSCLE CELLS FROM BOVINE AORTA, BIOCHIMICA ET BIOPHYSICA ACTA 544: 514 (1978). (10.1016/0304-4165(78)90326-4) / BIOCHIMICA ET BIOPHYSICA ACTA (1978)
GARDAIS, A, GLYCOSAMINOGLYCAN (GAG) DISTRIBUTION IN AORTIC WALL FROM 5 SPECIES, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY 44: 507 (1973). / COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY (1973)
GIMBRONE, M.A., HUMAN VASCULAR ENDOTHELIAL CELLS IN CULTURE - GROWTH AND DNA-SYNTHESIS, JOURNAL OF CELL BIOLOGY 60: 673 (1974). (10.1083/jcb.60.3.673) / JOURNAL OF CELL BIOLOGY (1974)
GLASSBERG, M.K., CULTURED ENDOTHELIAL-CELLS DERIVED FROM THE HUMAN ILIAC ARTERIES, IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION 18: 859 (1982). / IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION (1982)
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JOHNSON, A.R., HUMAN PULMONARY ENDOTHELIAL-CELLS IN CULTURE - ACTIVITIES OF CELLS FROM ARTERIES AND CELLS FROM VEINS, JOURNAL OF CLINICAL INVESTIGATION 65: 841 (1980). (10.1172/JCI109736) / JOURNAL OF CLINICAL INVESTIGATION (1980)
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KRAEMER, P.M., HIGH MOLECULAR-WEIGHT HEPARAN SULFATE FROM CELL-SURFACE, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 56: 423 (1974). (10.1016/0006-291X(74)90859-6) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1974)
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10.1126/science.7355268
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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 2:37 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 5:52 p.m.)
Indexed	1 week ago (Aug. 24, 2025, 7:03 p.m.)
Issued	41 years, 9 months ago (Nov. 11, 1983)
Published	41 years, 9 months ago (Nov. 11, 1983)
Published Print	41 years, 9 months ago (Nov. 11, 1983)

Funders 0

None

BibTeX

@article{Thornton_1983, title={Human Endothelial Cells: Use of Heparin in Cloning and Long-Term Serial Cultivation}, volume={222}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.6635659}, DOI={10.1126/science.6635659}, number={4624}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Thornton, Susan C. and Mueller, Stephen N. and Levine, Elliot M.}, year={1983}, month=nov, pages={623–625} }

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