DOI: 10.1126/science.6419347. Purification and Sequence Analysis of Bioactive Atrial Peptides (Atriopeptins)

Purification and Sequence Analysis of Bioactive Atrial Peptides (Atriopeptins)

10.1126/science.6419347

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Mammalian cardiac atria have several biologically active peptides that exert profound effects on sodium excretion, urine volume, and smooth muscle tone. In the present study two such peptides of low molecular weight were purified and separated from each other on the basis of differences in charge, hydrophobicity, and biological profile. The first peptide, designated atriopeptin I, exhibits natriuretic and diuretic activity and selectivity relaxes intestinal smooth muscle but not vascular smooth muscle strips. The second peptide, atriopeptin II, is a potent natriuretic and diuretic that relaxes both intestinal and vascular strips. Sequence analysis of atriopeptin I indicates that it is composed of 21 amino acids, of which serine and glycine residues predominate. The amino terminal sequence of atriopeptin II up to residue 21 is the same as that of atriopeptin I, with the addition of the Phe-Arg extension at the carboxyl terminus. Both peptides appear to be derived from a common high molecular weight precursor (designated atriopeptigen); their biological selectivity and potency may be determined by the site of carboxyl terminal cleavage.

Bibliography

Currie, M. G., Geller, D. M., Cole, B. R., Siegel, N. R., Fok, K. F., Adams, S. P., Eubanks, S. R., Galluppi, G. R., & Needleman, P. (1984). Purification and Sequence Analysis of Bioactive Atrial Peptides (Atriopeptins). Science, 223(4631), 67â69.

Authors 9

Mark G. Currie (first)
David M. Geller (additional)
Barbara R. Cole (additional)
Ned R. Siegel (additional)
Kam F. Fok (additional)
Steven P. Adams (additional)
Shad R. Eubanks (additional)
Gerald R. Galluppi (additional)
Philip Needleman (additional)

References 14 Referenced 398

Currie, M. G., Proceedings of the National Academy of Sciences of the United States of America 81: 1230 (1984). / Proceedings of the National Academy of Sciences of the United States of America (1984)
10.1126/science.6857267
DEBOLD, A.J., A RAPID AND POTENT NATRIURETIC RESPONSE TO INTRAVENOUS-INJECTION OF ATRIAL MYOCARDIAL EXTRACT IN RATS, LIFE SCIENCES 28: 89 (1981). (10.1016/0024-3205(81)90370-2) / LIFE SCIENCES (1981)
DEBOLD, A.J., CARDIONATRIN-I - A NOVEL HEART PEPTIDE WITH POTENT DIURETIC AND NATRIURETIC PROPERTIES, LIFE SCIENCES 33: 297 (1983). (10.1016/0024-3205(83)90390-9) / LIFE SCIENCES (1983)
GRAMMER, R.T., RAT ATRIAL NATRIURETIC FACTOR - PURIFICATION AND VASORELAXANT ACTIVITY, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 116: 696 (1983). (10.1016/0006-291X(83)90581-8) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1983)
HAYASHI, R, METHODS IN ENZYMOLOGY 47: 84 (1977). (10.1016/0076-6879(77)47010-1) / METHODS IN ENZYMOLOGY (1977)
HILL, D.W., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF AMINO-ACIDS IN THE PICOMOLE RANGE, ANALYTICAL CHEMISTRY 51: 1338 (1979). (10.1021/ac50044a055) / ANALYTICAL CHEMISTRY (1979)
HUNKAPILLER, M.W., HIGH-SENSITIVITY SEQUENCING WITH A GAS-PHASE SEQUENATOR, METHODS IN ENZYMOLOGY 91: 399 (1983). (10.1016/S0076-6879(83)91038-8) / METHODS IN ENZYMOLOGY (1983)
HUNKAPILLER, M.W., ANALYSIS OF PHENYLTHIOHYDANTOINS BY ULTRASENSITIVE GRADIENT HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, METHODS IN ENZYMOLOGY 91: 486 (1983). (10.1016/S0076-6879(83)91045-5) / METHODS IN ENZYMOLOGY (1983)
KEELER, R, ATRIAL NATRIURETIC FACTOR HAS A DIRECT, PROSTAGLANDIN-INDEPENDENT ACTION ON KIDNEYS, CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY 60: 1078 (1982). (10.1139/y82-155) / CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (1982)
SANTOME, J.A., C-TERMINAL SEQUENCE OF AMINO ACIDS IN BOVINE GROWTH HORMONE, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 20: 482 (1965). (10.1016/0006-291X(65)90604-2) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1965)
SONNENBERG, H, INTRA-RENAL LOCALIZATION OF THE NATRIURETIC EFFECT OF CARDIAC ATRIAL EXTRACT, CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY 60: 1149 (1982). (10.1139/y82-166) / CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (1982)
THIBAULT, G, HYPERTENSION 5: I75 (1983). / HYPERTENSION (1983)
TRIPPODO, N.C., PARTIAL CHEMICAL CHARACTERIZATION OF A NATRIURETIC SUBSTANCE IN RAT ATRIAL HEART-TISSUE, PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE 170: 502 (1982). (10.3181/00379727-170-41465) / PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE (1982)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 3:41 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 6:16 p.m.)
Indexed	5 days, 10 hours ago (Aug. 23, 2025, 1:02 a.m.)
Issued	41 years, 7 months ago (Jan. 6, 1984)
Published	41 years, 7 months ago (Jan. 6, 1984)
Published Print	41 years, 7 months ago (Jan. 6, 1984)

Funders 0

None

BibTeX

@article{Currie_1984, title={Purification and Sequence Analysis of Bioactive Atrial Peptides (Atriopeptins)}, volume={223}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.6419347}, DOI={10.1126/science.6419347}, number={4631}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Currie, Mark G. and Geller, David M. and Cole, Barbara R. and Siegel, Ned R. and Fok, Kam F. and Adams, Steven P. and Eubanks, Shad R. and Galluppi, Gerald R. and Needleman, Philip}, year={1984}, month=jan, pages={67–69} }

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