DOI: 10.1126/science.6328661. Adaptation of Lymphadenopathy Associated Virus (LAV) to Replication in EBV-Transformed B Lymphoblastoid Cell Lines

Adaptation of Lymphadenopathy Associated Virus (LAV) to Replication in EBV-Transformed B Lymphoblastoid Cell Lines

10.1126/science.6328661

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A strain of lymphadenopathy associated retrovirus (LAV) passaged in vitro was used to infect a lymphoblastoid cell line obtained by transformation with Epstein-Barr virus of B lymphocytes from a healthy donor. The virus produced from this line (B-LAV) was also able to grow at a high rate in some other lymphoblastoid lines and in a Burkitt lymphoma line. This adapted strain retained the biochemical, ultrastructural, and antigenic characteristics of the original strain, as well as its tropism for normal T4 + lymphocytes. It is thus possible to grow LAV in large quantities that can be used for the preparation of diagnostic reagents. The interaction between such a human retrovirus and Epstein-Barr virus, a DNA virus, may have some implication for the pathology of the acquired immunodeficiency syndrome and related diseases.

Bibliography

Montagnier, L., Gruest, J., Chamaret, S., Dauguet, C., Axler, C., GuÃ©tard, D., Nugeyre, M. T., BarrÃ©-Sinoussi, F., Chermann, J.-C., Brunet, J. B., Klatzmann, D., & Gluckman, J. C. (1984). Adaptation of Lymphadenopathy Associated Virus (LAV) to Replication in EBV-Transformed B Lymphoblastoid Cell Lines. Science, 225(4657), 63â66.

Authors 12

L. Montagnier (first)
J. Gruest (additional)
S. Chamaret (additional)
C. Dauguet (additional)
C. Axler (additional)
D. Guétard (additional)
M. T. Nugeyre (additional)
F. Barré-Sinoussi (additional)
J.-C. Chermann (additional)
J. B. Brunet (additional)
D. Klatzmann (additional)
J. C. Gluckman (additional)

References 18 Referenced 276

Morbidity and Mortality Weekly Report 31: 277 (1982). / Morbidity and Mortality Weekly Report (1982)
10.1126/science.6189183
BAYLISS, G.J., EPSTEIN-BARR VIRUS-INDUCED CELL-FUSION, NATURE 287: 164 (1980). (10.1038/287164a0) / NATURE (1980)
CHEN, ISY, LONG TERMINAL REPEATS OF HUMAN T-CELL LEUKEMIA VIRUS-II GENOME DETERMINE TARGET-CELL SPECIFICITY, NATURE 309: 276 (1984). (10.1038/309276a0) / NATURE (1984)
DESGRANGES, C, BIOMEDICINE EXPRESS 25: 349 (1976). / BIOMEDICINE EXPRESS (1976)
Ellrodt, A., Lancet 323: 1383 (1984). (10.1016/S0140-6736(84)91877-4) / Lancet (1984)
10.1126/science.6328660
KLEIN, E, T-CELL RESPONSE TO B-CELLS AND EPSTEIN-BARR VIRUS-ANTIGENS IN INFECTIOUS MONONUCLEOSIS, CANCER RESEARCH 41: 4210 (1981). / CANCER RESEARCH (1981)
LASKY, R.D., POSSIBLE DNA RNA TUMOR-VIRUS INTERACTION IN HUMAN LYMPHOMAS - EXPRESSION OF RETROVIRAL PROTEINS IN RAMOS LYMPHOMA LINES IS ENHANCED AFTER CONVERSION WITH EPSTEIN-BARR VIRUS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 81: 33 (1984). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1984)
LEANDERSON, T, B-CELL GROWTH-FACTOR - DISTINCTION FROM T-CELL GROWTH-FACTOR AND B-CELL MATURATION FACTOR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 79: 7455 (1982). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1982)
LENZ, J, DETERMINATION OF THE LEUKEMOGENICITY OF A MURINE RETROVIRUS BY SEQUENCES WITHIN THE LONG TERMINAL REPEAT, NATURE 308: 467 (1984). (10.1038/308467a0) / NATURE (1984)
MONTAGNIER, L, A NEW TYPE OF RETROVIRUS ISOLATED FROM PATIENTS PRESENTING WITH LYMPHADENOPATHY AND ACQUIRED IMMUNE-DEFICIENCY SYNDROME - STRUCTURAL AND ANTIGENIC RELATEDNESS WITH EQUINE INFECTIOUS-ANEMIA VIRUS, ANNALES DE VIROLOGIE 135: 119 (1984). / ANNALES DE VIROLOGIE (1984)
Montagnier L. Human T-Cell Leukemia/Lymphoma Virus (1984).
MONTAGNIER L unpublished data.
PEARSON, G.R., IDENTIFICATION OF POLYPEPTIDE COMPONENTS OF THE EPSTEIN-BARR VIRUS EARLY ANTIGEN COMPLEX WITH MONOCLONAL-ANTIBODIES, JOURNAL OF VIROLOGY 47: 193 (1983). (10.1128/jvi.47.1.193-201.1983) / JOURNAL OF VIROLOGY (1983)
10.1126/science.6200935
Rey, M. A., Biochemical and Biophysical Research Communications 121: 126 (1984). (10.1016/0006-291X(84)90696-X) / Biochemical and Biophysical Research Communications (1984)
VILMER, E, ISOLATION OF NEW LYMPHOTROPIC RETROVIRUS FROM 2 SIBLINGS WITH HEMOPHILIA-B, ONE WITH AIDS, LANCET 1: 753 (1984). / LANCET (1984)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 3:41 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 5:27 p.m.)
Indexed	1 month, 3 weeks ago (July 4, 2025, 2:11 a.m.)
Issued	41 years, 1 month ago (July 6, 1984)
Published	41 years, 1 month ago (July 6, 1984)
Published Print	41 years, 1 month ago (July 6, 1984)

Funders 0

None

BibTeX

@article{Montagnier_1984, title={Adaptation of Lymphadenopathy Associated Virus (LAV) to Replication in EBV-Transformed B Lymphoblastoid Cell Lines}, volume={225}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.6328661}, DOI={10.1126/science.6328661}, number={4657}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Montagnier, L. and Gruest, J. and Chamaret, S. and Dauguet, C. and Axler, C. and Guétard, D. and Nugeyre, M. T. and Barré-Sinoussi, F. and Chermann, J.-C. and Brunet, J. B. and Klatzmann, D. and Gluckman, J. C.}, year={1984}, month=jul, pages={63–66} }

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