DOI: 10.1126/science.6322302. Increased Phosphorylation of Myosin Light Chain Kinase After an Increase in Cyclic AMP in Intact Smooth Muscle

Increased Phosphorylation of Myosin Light Chain Kinase After an Increase in Cyclic AMP in Intact Smooth Muscle

10.1126/science.6322302

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The role of cyclic adenosine monophosphate-mediated phosphorylation of myosin light chain kinase in relaxing smooth muscle was examined. The kinase was immunoprecipitated from tissue extracts and the phosphate content was determined. The addition of forskolin to resting or methacholine-contracted muscles resulted in an increase in myosin light chain kinase phosphorylation and a relaxation of contracted muscles. These findings suggest that phosphorylation of myosin light chain kinase is one of the reactions in the process by which cyclic adenosine monophosphate causes relaxation of smooth muscle.

Bibliography

de Lanerolle, P., Nishikawa, M., Yost, D. A., & Adelstein, R. S. (1984). Increased Phosphorylation of Myosin Light Chain Kinase After an Increase in Cyclic AMP in Intact Smooth Muscle. Science, 223(4643), 1415â1417.

Authors 4

Primal de Lanerolle (first)
Masakatsu Nishikawa (additional)
David A. Yost (additional)
Robert S. Adelstein (additional)

References 24 Referenced 102

ADELSTEIN, R.S., REGULATION AND KINETICS OF THE ACTIN-MYOSIN-ATP INTERACTION, ANNUAL REVIEW OF BIOCHEMISTRY 49: 921 (1980). (10.1146/annurev.bi.49.070180.004421) / ANNUAL REVIEW OF BIOCHEMISTRY (1980)
AKSOY, M.O., ROLE OF CA-2+ AND MYOSIN LIGHT CHAIN PHOSPHORYLATION IN REGULATION OF SMOOTH-MUSCLE, AMERICAN JOURNAL OF PHYSIOLOGY 242: C109 (1982). (10.1152/ajpcell.1982.242.1.C109) / AMERICAN JOURNAL OF PHYSIOLOGY (1982)
BARRON, J.T., REVERSIBLE PHOSPHORYLATION AND DEPHOSPHORYLATION OF THE 20,000-DALTON LIGHT CHAIN OF MYOSIN DURING THE CONTRACTION-RELAXATION-CONTRACTION CYCLE OF ARTERIAL SMOOTH-MUSCLE, JOURNAL OF BIOLOGICAL CHEMISTRY 255: 6238 (1980). (10.1016/S0021-9258(18)43729-5) / JOURNAL OF BIOLOGICAL CHEMISTRY (1980)
10.1126/science.184530
CONTI, M.A., THE RELATIONSHIP BETWEEN CALMODULIN BINDING AND PHOSPHORYLATION OF SMOOTH-MUSCLE MYOSIN KINASE BY THE CATALYTIC SUBUNIT OF 3'-5' CAMP-DEPENDENT PROTEIN-KINASE, JOURNAL OF BIOLOGICAL CHEMISTRY 256: 3178 (1981). (10.1016/S0021-9258(19)69586-4) / JOURNAL OF BIOLOGICAL CHEMISTRY (1981)
DABROWSKA, R, MODULATOR PROTEIN AS A COMPONENT OF MYOSIN LIGHT CHAIN KINASE FROM CHICKEN GIZZARD, BIOCHEMISTRY 17: 253 (1978). (10.1021/bi00595a010) / BIOCHEMISTRY (1978)
DELANEROLLE, P, J BIOL CHEM 255: 9993 (1980). (10.1016/S0021-9258(18)43491-6) / J BIOL CHEM (1980)
DELANEROLLE, P, MYOSIN PHOSPHORYLATION, AGONIST CONCENTRATION AND CONTRACTION OF TRACHEAL SMOOTH-MUSCLE, NATURE 298: 871 (1982). (10.1038/298871a0) / NATURE (1982)
DELANEROLLE, P, CHARACTERIZATION OF ANTIBODIES TO SMOOTH-MUSCLE MYOSIN KINASE AND THEIR USE IN LOCALIZING MYOSIN KINASE IN NON-MUSCLE CELLS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 78: 4738 (1981). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1981)
DELANEROLLE P unpublished data.
HARPER, J.F., FEMTOMOLE SENSITIVE RADIOIMMUNOASSAY FOR CYCLIC-AMP AND CYCLIC-GMP AFTER 2'0 ACETYLATION BY ACETIC-ANHYDRIDE IN AQUEOUS-SOLUTION, JOURNAL OF CYCLIC NUCLEOTIDE RESEARCH 1: 207 (1975). / JOURNAL OF CYCLIC NUCLEOTIDE RESEARCH (1975)
HILL, A.B., PRINCIPLES MED STAT: 146 (1971). / PRINCIPLES MED STAT (1971)
INNES, I.R., PHARMACOL BASIS THER: 514 (1975). / PHARMACOL BASIS THER (1975)
KATSUKI, S, REGULATION OF ADENOSINE CYCLIC 3',5'-MONOPHOSPHATE AND GUANOSINE CYCLIC 3',5'-MONOPHOSPHATE LEVELS AND CONTRACTILITY IN BOVINE TRACHEAL SMOOTH-MUSCLE, MOLECULAR PHARMACOLOGY 13: 330 (1977). / MOLECULAR PHARMACOLOGY (1977)
KERRICK, WGL, INHIBITION OF SMOOTH-MUSCLE TENSION BY CYCLIC AMP-DEPENDENT PROTEIN-KINASE, NATURE 292: 253 (1981). (10.1038/292253a0) / NATURE (1981)
MILLER, J.R., THE ROLE OF MYOSIN LIGHT CHAIN KINASE PHOSPHORYLATION IN BETA-ADRENERGIC RELAXATION OF TRACHEAL SMOOTH-MUSCLE, MOLECULAR PHARMACOLOGY 24: 235 (1983). / MOLECULAR PHARMACOLOGY (1983)
MUELLER, E, ROLE OF INTRACELLULAR CA-2+ SEQUESTRATION IN BETA-ADRENERGIC RELAXATION OF A SMOOTH-MUSCLE, NATURE 281: 682 (1979). (10.1038/281682a0) / NATURE (1979)
NISHIKORI, K, EFFECTS OF RELAXIN ON RAT UTERINE MYOSIN LIGHT CHAIN KINASE-ACTIVITY AND MYOSIN LIGHT CHAIN PHOSPHORYLATION, JOURNAL OF BIOLOGICAL CHEMISTRY 258: 2468 (1983). (10.1016/S0021-9258(18)32949-1) / JOURNAL OF BIOLOGICAL CHEMISTRY (1983)
RINARD, G.A., DEPRESSED CYCLIC-AMP LEVELS IN AIRWAY SMOOTH-MUSCLE FROM ASTHMATIC DOGS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 76: 1472 (1979). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1979)
SEAMON, K.B., FORSKOLIN - A UNIQUE DITERPENE ACTIVATOR OF CYCLIC AMP-GENERATING SYSTEMS, JOURNAL OF CYCLIC NUCLEOTIDE RESEARCH 7: 201 (1981). / JOURNAL OF CYCLIC NUCLEOTIDE RESEARCH (1981)
SILVER, P.J., REGULATION OF MYOSIN LIGHT CHAIN AND PHOSPHORYLASE PHOSPHORYLATION IN TRACHEAL SMOOTH-MUSCLE, JOURNAL OF BIOLOGICAL CHEMISTRY 257: 6145 (1982). (10.1016/S0021-9258(20)65118-3) / JOURNAL OF BIOLOGICAL CHEMISTRY (1982)
TORPHY, T.J., CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN-KINASES IN AIRWAY SMOOTH-MUSCLE, JOURNAL OF BIOLOGICAL CHEMISTRY 257: 11609 (1982). (10.1016/S0021-9258(18)33806-7) / JOURNAL OF BIOLOGICAL CHEMISTRY (1982)
WALSH, M.P., BOVINE STOMACH MYOSIN LIGHT CHAIN KINASE - PURIFICATION, CHARACTERIZATION, AND COMPARISON WITH THE TURKEY GIZZARD ENZYME, BIOCHEMISTRY 21: 6890 (1982). (10.1021/bi00269a041) / BIOCHEMISTRY (1982)
YOST D.A. unpublished data.

Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 3:41 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 5 p.m.)
Indexed	1 day, 14 hours ago (Sept. 4, 2025, 10:26 a.m.)
Issued	41 years, 5 months ago (March 30, 1984)
Published	41 years, 5 months ago (March 30, 1984)
Published Print	41 years, 5 months ago (March 30, 1984)

Funders 0

None

BibTeX

@article{de_Lanerolle_1984, title={Increased Phosphorylation of Myosin Light Chain Kinase After an Increase in Cyclic AMP in Intact Smooth Muscle}, volume={223}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.6322302}, DOI={10.1126/science.6322302}, number={4643}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={de Lanerolle, Primal and Nishikawa, Masakatsu and Yost, David A. and Adelstein, Robert S.}, year={1984}, month=mar, pages={1415–1417} }

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