DOI: 10.1126/science.6310754. Expression of a Platelet-Derived Growth Factor-Like Protein in Simian Sarcoma Virus Transformed Cells

Expression of a Platelet-Derived Growth Factor-Like Protein in Simian Sarcoma Virus Transformed Cells

10.1126/science.6310754

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The near identity of the partial amino acid sequence of human platelet-derived growth factor (PDGF) and that predicted for p28 sis , the putative transforming protein of the simian sarcoma virus (SSV), suggests expression of a growth factor activity may be central for transformation by SSV. It is now reported that SSV-transformed cells but not control cells contain a growth factor activity that is identical to PDGF in immunoassay, in mitogenic dose response, and in specific mitogenic activity. The protein immunoprecipitated by antiserum to human PDGF has an apparent molecular weight of 20,000, identical to that of p20 sis , the putative intracellular degradation product of p28 sis . The results support the concept that expression of a PDGF-like molecule, which appears to be the product of the viral- sis gene, is responsible for the abnormal regulation of growth is SSV-transformed cells.

Bibliography

Deuel, T. F., Huang, J. S., Huang, S. S., Stroobant, P., & Waterfield, M. D. (1983). Expression of a Platelet-Derived Growth Factor-Like Protein in Simian Sarcoma Virus Transformed Cells. Science, 221(4618), 1348â1350.

Authors 5

Thomas F. Deuel (first)
Jung San Huang (additional)
Shuan Shian Huang (additional)
Paul Stroobant (additional)
Michael D. Waterfield (additional)

References 32 Referenced 166

Antoniades, H. N., Proceedings of the National Academy of Sciences of the United States of America 78: 7314 (1981). / Proceedings of the National Academy of Sciences of the United States of America (1981)
ANTONIADES, H.N., PURIFICATION OF HUMAN PLATELET-DERIVED GROWTH-FACTOR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 76: 1809 (1979). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1979)
10.1126/science.6844921
BALK, S.D., CALCIUM AS A REGULATOR OF PROLIFERATION OF NORMAL, BUT NOT OF TRANSFORMED, CHICKEN FIBROBLASTS IN A PLASMA-CONTAINING MEDIUM, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 68: 271 (1971). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1971)
BALK, S.D., ROLES OF CALCIUM, SERUM, PLASMA, AND FOLIC-ACID IN CONTROL OF PROLIFERATION OF NORMAL AND ROUS-SARCOMA VIRUS-INFECTED CHICKEN FIBROBLASTS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 70: 675 (1973). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1973)
Cassel, D., Proceedings of the National Academy of Sciences of the United States of America 80: 6224 (1983). / Proceedings of the National Academy of Sciences of the United States of America (1983)
DEUEL, T.F., HUMAN PLATELET-DERIVED GROWTH-FACTOR - PURIFICATION AND RESOLUTION INTO 2 ACTIVE PROTEIN-FRACTIONS, JOURNAL OF BIOLOGICAL CHEMISTRY 256: 8896 (1981). (10.1016/S0021-9258(19)52482-6) / JOURNAL OF BIOLOGICAL CHEMISTRY (1981)
DEUEL, T.F., CHEMOTAXIS OF MONOCYTES AND NEUTROPHILS TO PLATELET-DERIVED GROWTH-FACTOR, JOURNAL OF CLINICAL INVESTIGATION 69: 1046 (1982). (10.1172/JCI110509) / JOURNAL OF CLINICAL INVESTIGATION (1982)
DEUEL, T.F., JOURNAL OF SUPRAMOLECULAR STRUCTURE AND CELLULAR BIOCHEMISTRY 17 5: 128 (1981). / JOURNAL OF SUPRAMOLECULAR STRUCTURE AND CELLULAR BIOCHEMISTRY (1981)
Deuel T. F. Progress in Hematology 13 (1983).
Devare, S. G., Proceedings of the National Academy of Sciences of the United States of America 80: 731 (1983). / Proceedings of the National Academy of Sciences of the United States of America (1983)
10.1126/science.6304883
EK, B, STIMULATION OF TYROSINE-SPECIFIC PHOSPHORYLATION BY PLATELET-DERIVED GROWTH-FACTOR, NATURE 295: 419 (1982). (10.1038/295419a0) / NATURE (1982)
EVA, A, CELLULAR GENES ANALOGOUS TO RETROVIRAL ONC GENES ARE TRANSCRIBED IN HUMAN-TUMOR CELLS, NATURE 295: 116 (1982). (10.1038/295116a0) / NATURE (1982)
Grotendorst, G. R., Proceedings of the National Academy of Sciences of the United States of America 78: 3669 (1981). / Proceedings of the National Academy of Sciences of the United States of America (1981)
HELDIN, C.H., PLATELET-DERIVED GROWTH-FACTOR - ISOLATION BY A LARGE-SCALE PROCEDURE AND ANALYSIS OF SUBUNIT COMPOSITION, BIOCHEMICAL JOURNAL 193: 907 (1981). (10.1042/bj1930907) / BIOCHEMICAL JOURNAL (1981)
HELDIN, C.H., PLATELET-DERIVED GROWTH-FACTOR - PURIFICATION AND PARTIAL CHARACTERIZATION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 76: 3722 (1979). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1979)
Huang, J. S., Differentiation and Function of Hematopoietic Cell Surfaces: 225 (1982). / Differentiation and Function of Hematopoietic Cell Surfaces (1982)
HUANG, J.S., HUMAN PLATELET-DERIVED GROWTH-FACTOR - RADIOIMMUNOASSAY AND DISCOVERY OF A SPECIFIC PLASMA-BINDING PROTEIN, JOURNAL OF CELL BIOLOGY 97: 383 (1983). (10.1083/jcb.97.2.383) / JOURNAL OF CELL BIOLOGY (1983)
KOHLER, N, PLATELETS AS A SOURCE OF FIBROBLAST GROWTH-PROMOTING ACTIVITY, EXPERIMENTAL CELL RESEARCH 87: 297 (1974). (10.1016/0014-4827(74)90484-4) / EXPERIMENTAL CELL RESEARCH (1974)
NISHIMURA, J, STIMULATION OF PROTEIN-PHOSPHORYLATION IN SWISS MOUSE 3T3-CELLS BY HUMAN-PLATELET DERIVED GROWTH-FACTOR, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 103: 355 (1981). (10.1016/0006-291X(81)91700-9) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1981)
NISHIMURA, J, PLATELET-DERIVED GROWTH-FACTOR STIMULATES THE PHOSPHORYLATION OF RIBOSOMAL PROTEIN-S6, FEBS LETTERS 156: 130 (1983). (10.1016/0014-5793(83)80263-4) / FEBS LETTERS (1983)
NISHIMURA, J, PLATELET-DERIVED GROWTH-FACTOR STIMULATES TYROSINE-SPECIFIC PROTEIN-KINASE ACTIVITY IN SWISS MOUSE 3T3 CELL-MEMBRANES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 79: 4303 (1982). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1982)
RAINES, E.W., PLATELET-DERIVED GROWTH-FACTOR .1. HIGH-YIELD PURIFICATION AND EVIDENCE FOR MULTIPLE FORMS, JOURNAL OF BIOLOGICAL CHEMISTRY 257: 5154 (1982). (10.1016/S0021-9258(18)34649-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1982)
ROBBINS K.C. COMMUNICATION.
ROSS, R, PLATELET-DERIVED GROWTH-FACTOR, CELL 14: 203 (1978). (10.1016/0092-8674(78)90107-1) / CELL (1978)
ROSS, R, PLATELET-DEPENDENT SERUM FACTOR THAT STIMULATES PROLIFERATION OF ARTERIAL SMOOTH-MUSCLE CELLS INVITRO, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 71: 1207 (1974). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1974)
Rozengurt, E., Cell 34: 265 (1983). (10.1016/0092-8674(83)90157-5) / Cell (1983)
SENIOR, R.M., CHEMOTACTIC ACTIVITY OF PLATELET ALPHA-GRANULE PROTEINS FOR FIBROBLASTS, JOURNAL OF CELL BIOLOGY 96: 382 (1983). (10.1083/jcb.96.2.382) / JOURNAL OF CELL BIOLOGY (1983)
SEPPA, H, PLATELET-DERIVED GROWTH-FACTOR IS CHEMOTACTIC FOR FIBROBLASTS, JOURNAL OF CELL BIOLOGY 92: 584 (1982). (10.1083/jcb.92.2.584) / JOURNAL OF CELL BIOLOGY (1982)
USHIRO, H, IDENTIFICATION OF PHOSPHOTYROSINE AS A PRODUCT OF EPIDERMAL GROWTH FACTOR-ACTIVATED PROTEIN-KINASE IN A-431 CELL-MEMBRANES, JOURNAL OF BIOLOGICAL CHEMISTRY 255: 8363 (1980). (10.1016/S0021-9258(18)43497-7) / JOURNAL OF BIOLOGICAL CHEMISTRY (1980)
10.1038/304035a0

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 2:37 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 5:04 p.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 9:15 a.m.)
Issued	41 years, 11 months ago (Sept. 30, 1983)
Published	41 years, 11 months ago (Sept. 30, 1983)
Published Print	41 years, 11 months ago (Sept. 30, 1983)

Funders 0

None

BibTeX

@article{Deuel_1983, title={Expression of a Platelet-Derived Growth Factor-Like Protein in Simian Sarcoma Virus Transformed Cells}, volume={221}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.6310754}, DOI={10.1126/science.6310754}, number={4618}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Deuel, Thomas F. and Huang, Jung San and Huang, Shuan Shian and Stroobant, Paul and Waterfield, Michael D.}, year={1983}, month=sep, pages={1348–1350} }

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