DOI: 10.1126/science.6199844. Heparin Affinity: Purification of a Tumor-Derived Capillary Endothelial Cell Growth Factor

Heparin Affinity: Purification of a Tumor-Derived Capillary Endothelial Cell Growth Factor

10.1126/science.6199844

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A tumor-derived growth factor that stimulates the proliferation of capillary endothelial cells has a very strong affinity for heparin. This heparin affinity makes it possible to purify the growth factor to a single-band preparation in a rapid two-step procedure. The purified growth factor is a cationic polypeptide, has a molecular weight of about 18,000, and stimulates capillary endothelial cell proliferation at a concentration of about 1 nanogram per milliliter.

Bibliography

Shing, Y., Folkman, J., Sullivan, R., Butterfield, C., Murray, J., & Klagsbrun, M. (1984). Heparin Affinity: Purification of a Tumor-Derived Capillary Endothelial Cell Growth Factor. Science, 223(4642), 1296â1299.

Authors 6

Y. Shing (first)
J. Folkman (additional)
R. Sullivan (additional)
C. Butterfield (additional)
J. Murray (additional)
M. Klagsbrun (additional)

References 20 Referenced 675

AZIZKHAN, J.C., CANCER RES 43: 328 (1983). / CANCER RES (1983)
BUONASSISI, V, ENZYMATIC DEGRADATION OF HEPARIN-RELATED MUCOPOLYSACCHARIDES FROM SURFACE OF ENDOTHELIAL CELL-CULTURES, BIOCHIMICA ET BIOPHYSICA ACTA 385: 1 (1975). (10.1016/0304-4165(75)90067-7) / BIOCHIMICA ET BIOPHYSICA ACTA (1975)
BUSCH, C, SURFACE-PROPERTIES OF CULTURED ENDOTHELIAL-CELLS, HAEMOSTASIS 8: 142 (1979). / HAEMOSTASIS (1979)
CAVALLO, T, TUMOR ANGIOGENESIS - RAPID INDUCTION OF ENDOTHELIAL MITOSES DEMONSTRATED BY AUTORADIOGRAPHY, JOURNAL OF CELL BIOLOGY 54: 408 (1972). (10.1083/jcb.54.2.408) / JOURNAL OF CELL BIOLOGY (1972)
FENSELAU, A, TUMOR ANGIOGENIC FACTOR - PURIFICATION FROM THE WALKER 256 RAT-TUMOR, JOURNAL OF BIOLOGICAL CHEMISTRY 256: 9605 (1981). (10.1016/S0021-9258(19)68805-8) / JOURNAL OF BIOLOGICAL CHEMISTRY (1981)
FOLKMAN, J, ADVANCES IN CANCER RESEARCH 19: 331 (1974). (10.1016/S0065-230X(08)60058-5) / ADVANCES IN CANCER RESEARCH (1974)
FOLKMAN, J, LONG-TERM CULTURE OF CAPILLARY ENDOTHELIAL-CELLS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 76: 5217 (1979). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1979)
10.1126/science.6192498
HIEBERT, L.M., OBSERVATION OF HEPARIN ON ENDOTHELIUM AFTER INJECTION, THROMBOSIS RESEARCH 8: 195 (1976). (10.1016/0049-3848(76)90262-0) / THROMBOSIS RESEARCH (1976)
KEEGAN, A, PURIFIED TUMOR ANGIOGENESIS FACTOR ENHANCES PROLIFERATION OF CAPILLARY, BUT NOT AORTIC, ENDOTHELIAL-CELLS INVITRO, JOURNAL OF CELL SCIENCE 55: 261 (1982). (10.1242/jcs.55.1.261) / JOURNAL OF CELL SCIENCE (1982)
KLAGSBRUN, M, STIMULATION OF DNA-SYNTHESIS AND CELL-DIVISION IN CHONDROCYTES AND 3T3 CELLS BY A GROWTH-FACTOR ISOLATED FROM CARTILAGE, EXPERIMENTAL CELL RESEARCH 105: 99 (1977). (10.1016/0014-4827(77)90155-0) / EXPERIMENTAL CELL RESEARCH (1977)
KRAMER, P.M., BIOCHEMISTRY-US 10: 1445 (1971). (10.1021/bi00784a027) / BIOCHEMISTRY-US (1971)
10.1038/227680a0
OAKLEY, B.R., A SIMPLIFIED ULTRASENSITIVE SILVER STAIN FOR DETECTING PROTEINS IN POLYACRYLAMIDE GELS, ANALYTICAL BIOCHEMISTRY 105: 361 (1980). (10.1016/0003-2697(80)90470-4) / ANALYTICAL BIOCHEMISTRY (1980)
RIFKIN, D.B., PATHOBIOLOGY ENDOTHE: 191 (1982). / PATHOBIOLOGY ENDOTHE (1982)
TAYLOR, S, PROTAMINE IS AN INHIBITOR OF ANGIOGENESIS, NATURE 297: 307 (1982). (10.1038/297307a0) / NATURE (1982)
10.1126/science.6635659
WASTESON, A, EFFECT OF A PLATELET ENDOGLYCOSIDASE ON CELL-SURFACE ASSOCIATED HEPARAN SULFATE OF HUMAN CULTURED ENDOTHELIAL AND GLIAL CELLS, THROMBOSIS RESEARCH 11: 309 (1977). (10.1016/0049-3848(77)90184-0) / THROMBOSIS RESEARCH (1977)
ZETTER, B.R., J AM DIABETES ASS 30: 24 (1981). / J AM DIABETES ASS (1981)
ZETTER, B.R., MIGRATION OF CAPILLARY ENDOTHELIAL-CELLS IS STIMULATED BY TUMOUR-DERIVED FACTORS, NATURE 285: 41 (1980). (10.1038/285041a0) / NATURE (1980)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 3:41 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 10:25 a.m.)
Indexed	3 weeks, 6 days ago (July 30, 2025, 11:18 a.m.)
Issued	41 years, 5 months ago (March 23, 1984)
Published	41 years, 5 months ago (March 23, 1984)
Published Print	41 years, 5 months ago (March 23, 1984)

Funders 0

None

BibTeX

@article{Shing_1984, title={Heparin Affinity: Purification of a Tumor-Derived Capillary Endothelial Cell Growth Factor}, volume={223}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.6199844}, DOI={10.1126/science.6199844}, number={4642}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Shing, Y. and Folkman, J. and Sullivan, R. and Butterfield, C. and Murray, J. and Klagsbrun, M.}, year={1984}, month=mar, pages={1296–1299} }

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