DOI: 10.1126/science.6192501. Neurofilament Accumulation Induced in Synapses by Leupeptin

Neurofilament Accumulation Induced in Synapses by Leupeptin

10.1126/science.6192501

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The hypothesis that the usual absence of neurofilaments in synaptic terminals is due to their degradation by the calcium-activated protease present in axoplasm was tested by injecting leupeptin, which inhibits the protease, into the optic tectum of goldfish kept at 15° and at 25°C. The resulting accumulation of neurofilaments in synaptic terminals provides in vivo evidence in support of the hypothesis. The significance of these results and the potential uses of this pharmacological tool are discussed.

Bibliography

Roots, B. I. (1983). Neurofilament Accumulation Induced in Synapses by Leupeptin. Science, 221(4614), 971â972.

Authors 1

Betty I. Roots (first)

References 28 Referenced 122

BAKER, P.F., DEPOLARIZATION AND CALCIUM ENTRY IN SQUID GIANT AXONS, JOURNAL OF PHYSIOLOGY-LONDON 218: 709 (1971). (10.1113/jphysiol.1971.sp009641) / JOURNAL OF PHYSIOLOGY-LONDON (1971)
BONDAR, R.L., NEUROFIBRILLAR CHANGES IN GOLDFISH (CARASSIUS-AURATUS L) BRAIN IN RELATION TO ENVIRONMENTAL-TEMPERATURE, EXPERIMENTAL BRAIN RESEARCH 30: 577 (1977). / EXPERIMENTAL BRAIN RESEARCH (1977)
BOYCOTT, B.B., SYNAPTIC STRUCTURE AND ITS ALTERATION WITH ENVIRONMENTAL TEMPERATURE - A STUDY BY LIGHT AND ELECTRON MICROSCOPY OF CENTRAL NERVOUS SYSTEM OF LIZARDS, PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES 154: 151 (1961). / PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES (1961)
COLONNIER, M, SYNAPTIC ORGANIZATION IN THE LATERAL GENICULATE NUCLEUS OF THE MONKEY, ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE 62: 333 (1964). (10.1007/BF00339284) / ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE (1964)
DROZ, B, AXONAL MIGRATION OF PROTEINS IN CENTRAL NERVOUS SYSTEM AND PERIPHERAL NERVES AS SHOWN BY RADIOAUTOGRAPHY, JOURNAL OF COMPARATIVE NEUROLOGY 121: 325 (1963). (10.1002/cne.901210304) / JOURNAL OF COMPARATIVE NEUROLOGY (1963)
GAMBETTI, P, BODIAN SILVER METHOD STAINS NEUROFILAMENT POLYPEPTIDES, SCIENCE 213: 1521 (1981). (10.1126/science.6169146) / SCIENCE (1981)
GILBERT, D.S., NEUROFILAMENT DISGUISE, DESTRUCTION AND DISCIPLINE, NATURE 256: 586 (1975). (10.1038/256586a0) / NATURE (1975)
GRAY, E.G., BASIS FOR SILVER STAINING OF SYNAPSES OF MAMMALIAN SPINAL CORD - A LIGHT AND ELECTRON MICROSCOPE STUDY, JOURNAL OF PHYSIOLOGY-LONDON 157: 581 (1961). (10.1113/jphysiol.1961.sp006744) / JOURNAL OF PHYSIOLOGY-LONDON (1961)
HODGKIN, A.L., MOVEMENTS OF LABELLED CALCIUM IN SQUID GIANT AXONS, JOURNAL OF PHYSIOLOGY-LONDON 138: 253 (1957). (10.1113/jphysiol.1957.sp005850) / JOURNAL OF PHYSIOLOGY-LONDON (1957)
HOFFMAN, P.N., SLOW COMPONENT OF AXONAL-TRANSPORT - IDENTIFICATION OF MAJOR STRUCTURAL POLYPEPTIDES OF AXON AND THEIR GENERALITY AMONG MAMMALIAN NEURONS, JOURNAL OF CELL BIOLOGY 66: 351 (1975). (10.1083/jcb.66.2.351) / JOURNAL OF CELL BIOLOGY (1975)
HOLMES, W, Silver staining of nerve axons in paraffin sections, ANATOMICAL RECORD 86: 157 (1943). (10.1002/ar.1090860205) / ANATOMICAL RECORD (1943)
KAMAKURA, K, IDENTIFICATION OF CA2+-ACTIVATED NEUTRAL PROTEASE IN THE PERIPHERAL-NERVE AND ITS EFFECTS ON NEUROFILAMENT DEGENERATION, JOURNAL OF NEUROCHEMISTRY 40: 908 (1983). (10.1111/j.1471-4159.1983.tb08072.x) / JOURNAL OF NEUROCHEMISTRY (1983)
KARLSSON, U, FIXATION OF CENTRAL NERVOUS SYSTEM FOR ELECTRON MICROSCOPY BY ALDEHYDE PERFUSION .I. PRESERVATION WITH ALDEHYDE PERFUSATES VERSUS DIRECT PERFUSION WITH OSMIUM TETROXIDE WITH SPECIAL REFERENCE TO MEMBRANES AND EXTRACELLULAR SPACE, JOURNAL OF ULTRASTRUCTURE RESEARCH 12: 160 (1965). (10.1016/S0022-5320(65)80014-4) / JOURNAL OF ULTRASTRUCTURE RESEARCH (1965)
KATZ, B, FURTHER STUDY OF ROLE OF CALCIUM IN SYNAPTIC TRANSMISSION, JOURNAL OF PHYSIOLOGY-LONDON 207: 789 (1970). (10.1113/jphysiol.1970.sp009095) / JOURNAL OF PHYSIOLOGY-LONDON (1970)
LASEK, R.J., BIOL FUNCTIONS MICRO: 329 (1982). / BIOL FUNCTIONS MICRO (1982)
LASEK, R.J., CELL MOTILITY: 1021 (1976). / CELL MOTILITY (1976)
LASEK, R.J., INTERNATIONAL REVIEW OF NEUROBIOLOGY 13: 289 (1970). (10.1016/S0074-7742(08)60173-7) / INTERNATIONAL REVIEW OF NEUROBIOLOGY (1970)
LASEK, R.J., MECHANISMS REGULATIO: 161 (1977). / MECHANISMS REGULATIO (1977)
10.1126/science.176.4039.1127
MALIK, M.N., CALCIUM ACTIVATED PROTEOLYSIS OF FIBROUS PROTEINS IN CENTRAL NERVOUS-SYSTEM, LIFE SCIENCES 29: 795 (1981). (10.1016/0024-3205(81)90035-7) / LIFE SCIENCES (1981)
POTTER, H.D., J COMP NEUROL 155: 409 (1975). / J COMP NEUROL (1975)
POTTER, H.D., ALTERATIONS IN NEUROFIBRILLAR RINGS IN LIZARD BRAIN DURING COLD-ACCLIMATION, JOURNAL OF NEUROCYTOLOGY 2: 29 (1973). (10.1007/BF01099206) / JOURNAL OF NEUROCYTOLOGY (1973)
ROOTS, B.I., NEUROFILAMENTOUS CHANGES IN GOLDFISH (CARASSIUS-AURATUS L) BRAIN IN RELATION TO ENVIRONMENTAL-TEMPERATURE, JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY 36: 453 (1977). (10.1097/00005072-197705000-00004) / JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY (1977)
ROOTS, B.I., TRANSACTIONS OF THE AMERICAN SOCIETY FOR NEUROCHEMISTRY 14: 216 (1983). / TRANSACTIONS OF THE AMERICAN SOCIETY FOR NEUROCHEMISTRY (1983)
SCHLAEPFER, W.W., CHARACTERIZATION OF THE CALCIUM-INDUCED DISRUPTION OF NEUROFILAMENTS IN RAT PERIPHERAL-NERVE, BRAIN RESEARCH 168: 299 (1979). (10.1016/0006-8993(79)90171-9) / BRAIN RESEARCH (1979)
SCHLAEPFER, W.W., PROGRESS IN NEUROPATHOLOGY 4: 101 (1979). / PROGRESS IN NEUROPATHOLOGY (1979)
WEISS, P, EXPERIMENTS ON THE MECHANISM OF NERVE GROWTH, JOURNAL OF EXPERIMENTAL ZOOLOGY 107: 315 (1948). (10.1002/jez.1401070302) / JOURNAL OF EXPERIMENTAL ZOOLOGY (1948)
YOUNG, J.Z., PUBBLICAZIONI DELLA STAZIONE ZOOLOGICA DI NAPOLI 12: 425 (1933). / PUBBLICAZIONI DELLA STAZIONE ZOOLOGICA DI NAPOLI (1933)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 2:37 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:04 a.m.)
Indexed	1 year, 5 months ago (March 5, 2024, 3:24 p.m.)
Issued	41 years, 11 months ago (Sept. 2, 1983)
Published	41 years, 11 months ago (Sept. 2, 1983)
Published Print	41 years, 11 months ago (Sept. 2, 1983)

Funders 0

None

BibTeX

@article{Roots_1983, title={Neurofilament Accumulation Induced in Synapses by Leupeptin}, volume={221}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.6192501}, DOI={10.1126/science.6192501}, number={4614}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Roots, Betty I.}, year={1983}, month=sep, pages={971–972} }

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    "0036-8075",
    "1095-9203"
  ],
  "subject": [],
  "container-title-short": "Science",
  "published": {
    "date-parts": [
      [
        1983,
        9,
        2
      ]
    ]
  }
}