DOI: 10.1126/science.6163216. Induction of Hemoglobin Accumulation in Human K562 Cells by Hemin Is Reversible

Induction of Hemoglobin Accumulation in Human K562 Cells by Hemin Is Reversible

10.1126/science.6163216

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Twenty micromolar hemin causes no change in the rate of division of K562 cells but results in accumulation of 11 to 14 picograms of embryonic and fetal hemoglobins per cell. This effect is reversible, and hemoglobin induction in response to hemin, and loss of hemoglobin upon removal of hemin, can be cyclically repeated. The cells can be indefinitely subcultured in the presence of the inducer. Thus, the control of hemoglobin levels in K562 cells does not depend on irreversible differentiation.

Bibliography

Dean, A., Erard, F., Schneider, A. B., & Schechter, A. N. (1981). Induction of Hemoglobin Accumulation in Human K562 Cells by Hemin Is Reversible. Science, 212(4493), 459â461.

Authors 4

Ann Dean (first)
Francois Erard (additional)
Arthur B. Schneider (additional)
Alan N. Schechter (additional)

References 20 Referenced 89

ALTER, B.P., ELECTROPHORETIC SEPARATION OF HUMAN-EMBRYONIC GLOBIN DEMONSTRATES ALPHA-THALASSEMIA IN HUMAN-LEUKEMIA CELL-LINE K562, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 94: 843 (1980). (10.1016/0006-291X(80)91311-X) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1980)
ALTER, B.P., GLOBIN-SYNTHESIS IN MOUSE ERYTHROLEUKEMIA CELLS INVITRO - SWITCH IN BETA-CHAINS DUE TO INDUCING AGENT, BLOOD 50: 867 (1977). (10.1182/blood.V50.5.867.867) / BLOOD (1977)
ANDERSSON, L.C., INDUCTION OF ERYTHROID-DIFFERENTIATION IN THE HUMAN LEUKEMIA CELL-LINE K562, NATURE 278: 364 (1979). (10.1038/278364a0) / NATURE (1979)
BASSET, P, ISOELECTRIC-FOCUSING OF HUMAN HEMOGLOBIN - ITS APPLICATION TO SCREENING, TO CHARACTERIZATION OF 70 VARIANTS, AND TO STUDY OF MODIFIED FRACTIONS OF NORMAL HEMOGLOBINS, BLOOD 51: 971 (1978). (10.1182/blood.V51.5.971.971) / BLOOD (1978)
BENZ, E.J., NEW ENGLAND JOURNAL OF MEDICINE 297: 1371 (1977). (10.1056/NEJM197712222972504) / NEW ENGLAND JOURNAL OF MEDICINE (1977)
BENZ, E.J., EMBRYONIC-FETAL ERYTHROID CHARACTERISTICS OF A HUMAN-LEUKEMIC CELL-LINE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 77: 3509 (1980). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1980)
GUSELLA, J.F., HEMIN DOES NOT CAUSE COMMITMENT OF MURINE ERYTHROLEUKEMIA (MEL) CELLS TO TERMINAL DIFFERENTIATION, BLOOD 56: 481 (1980). (10.1182/blood.V56.3.481.481) / BLOOD (1980)
GUSELLA, J, COMMITMENT TO ERYTHROID DIFFERENTIATION BY FRIEND ERYTHROLEUKEMIA CELLS - STOCHASTIC ANALYSIS, CELL 9: 221 (1976). (10.1016/0092-8674(76)90113-6) / CELL (1976)
HOFFMAN, R, INDUCTION OF ERYTHROPOIETIC COLONIES IN A HUMAN CHRONIC MYELOGENOUS LEUKEMIA-CELL LINE, BLOOD 54: 1182 (1979). (10.1182/blood.V54.5.1182.1182) / BLOOD (1979)
HOFFMAN, R, HEMIN CONTROL OF HEME-BIOSYNTHESIS AND CATABOLISM IN A HUMAN-LEUKEMIA CELL-LINE, BLOOD 56: 567 (1980). (10.1182/blood.V56.3.567.567) / BLOOD (1980)
LOWENHAUPT, K, SYNTHESIS AND TURNOVER OF GLOBIN MESSENGER-RNA IN MURINE ERYTHROLEUKEMIA-CELLS INDUCED WITH HEMIN, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 76: 5173 (1979). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1979)
LOZZIO, C.B., HUMAN CHRONIC MYELOGENOUS LEUKEMIA CELL-LINE WITH POSITIVE PHILADELPHIA CHROMOSOME, BLOOD 45: 321 (1975). (10.1182/blood.V45.3.321.321) / BLOOD (1975)
MARKS, P.A., ERYTHROLEUKEMIC DIFFERENTIATION, ANNUAL REVIEW OF BIOCHEMISTRY 47: 419 (1978). (10.1146/annurev.bi.47.070178.002223) / ANNUAL REVIEW OF BIOCHEMISTRY (1978)
ROSS, J, INDUCTION OF GLOBIN MESSENGER-RNA ACCUMULATION BY HEMIN IN CULTURED ERYTHROLEUKEMIC CELLS, CELL 8: 513 (1976). (10.1016/0092-8674(76)90219-1) / CELL (1976)
ROVERA, G, EFFECT OF HEMIN ON EXPRESSION OF BETA-GLOBIN GENES IN FRIEND CELLS, FEBS LETTERS 81: 366 (1977). (10.1016/0014-5793(77)80556-5) / FEBS LETTERS (1977)
Rutherford, T., Proceedings of the National Academy of Sciences of the United States of America 78: 348 (1981). / Proceedings of the National Academy of Sciences of the United States of America (1981)
RUTHERFORD, T.R., DEFICIENT HEME SYNTHESIS AS THE CAUSE OF NON-INDUCIBILITY OF HEMOGLOBIN SYNTHESIS IN A FRIEND-ERYTHROLEUKEMIA CELL-LINE, CELL 16: 415 (1979). (10.1016/0092-8674(79)90017-5) / CELL (1979)
RUTHERFORD, T.R., K562 HUMAN-LEUKEMIC CELLS SYNTHESIZE EMBRYONIC HEMOGLOBIN IN RESPONSE TO HEMIN, NATURE 280: 164 (1979). (10.1038/280164a0) / NATURE (1979)
STAMATOYANNOPOU.G CELLULAR MOL REGULAT (1978).
WEATHERALL, D.J., RECENT DEVELOPMENTS IN THE MOLECULAR GENETICS OF HUMAN-HEMOGLOBIN, CELL 16: 467 (1979). (10.1016/0092-8674(79)90022-9) / CELL (1979)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 1:45 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 11:19 a.m.)
Indexed	1 year, 1 month ago (July 18, 2024, 3:49 a.m.)
Issued	44 years, 4 months ago (April 24, 1981)
Published	44 years, 4 months ago (April 24, 1981)
Published Print	44 years, 4 months ago (April 24, 1981)

Funders 0

None

BibTeX

@article{Dean_1981, title={Induction of Hemoglobin Accumulation in Human K562 Cells by Hemin Is Reversible}, volume={212}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.6163216}, DOI={10.1126/science.6163216}, number={4493}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Dean, Ann and Erard, Francois and Schneider, Arthur B. and Schechter, Alan N.}, year={1981}, month=apr, pages={459–461} }

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