DOI: 10.1126/science.424755. Distribution of Transmembrane Polypeptides in Freeze Fracture

Distribution of Transmembrane Polypeptides in Freeze Fracture

10.1126/science.424755

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Human erythrocytes have been freeze-fractured, and the polypeptides associated with the separate halves of the membrane bilayer have been analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The transmembrane proteins were differentially separated by the fracture process. Although sialoglycoproteins associated with the outer half of the membrane, the anion transport protein (band 3) mainly remained with the inner half of the membrane. Well-defined fragments of the sialoglycoproteins were produced by the freeze-fracture procedure, indicating that selected covalent bonds of these transmembrane proteins were broken.

Bibliography

Edwards, H. H., Mueller, T. J., & Morrison, M. (1979). Distribution of Transmembrane Polypeptides in Freeze Fracture. Science, 203(4387), 1343â1346.

Authors 3

Harold H. Edwards (first)
Thomas J. Mueller (additional)
Martin Morrison (additional)

References 36 Referenced 46

ARNON, R, METHODS IN ENZYMOLOGY 19: 226 (1970). (10.1016/0076-6879(70)19017-3) / METHODS IN ENZYMOLOGY (1970)
BACHI, T, BIOCHIM BIOPHYS ACTA 464: 635 (1977). (10.1016/0005-2736(77)90039-6) / BIOCHIM BIOPHYS ACTA (1977)
BEUTLER, E, REMOVAL OF LEUKOCYTES AND PLATELETS FROM WHOLE-BLOOD, JOURNAL OF LABORATORY AND CLINICAL MEDICINE 88: 328 (1976). / JOURNAL OF LABORATORY AND CLINICAL MEDICINE (1976)
BLUMENFELD, O.O., MODIFICATION AND INTRODUCTION OF A SPECIFIC RADIOACTIVE LABEL INTO ERYTHROCYTE-MEMBRANE SIALOGLYCOPROTEINS, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 48: 242 (1972). (10.1016/0006-291X(72)90369-5) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1972)
10.1111/j.1432-1033.1974.tb03599.x
BRANTON, D, FRACTURE FACES OF FROZEN MEMBRANES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 55: 1048 (1966). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1966)
BRETSCHER, M.S., HUMAN ERYTHROCYTE MEMBRANES - SPECIFIC LABELLING OF SURFACE PROTEINS, JOURNAL OF MOLECULAR BIOLOGY 58: 775 (1971). (10.1016/0022-2836(71)90039-8) / JOURNAL OF MOLECULAR BIOLOGY (1971)
ELGSAETER, A, INTRAMEMBRANE PARTICLE AGGREGATION IN ERYTHROCYTE-GHOSTS .2. INFLUENCE OF SPECTRIN AGGREGATION, BIOCHIMICA ET BIOPHYSICA ACTA 426: 101 (1976). (10.1016/0005-2736(76)90433-8) / BIOCHIMICA ET BIOPHYSICA ACTA (1976)
ENGSTROM L.H. THESIS U CALIFORNIA (1970).
FAIRBANKS, G, ELECTROPHORETIC ANALYSIS OF MAJOR POLYPEPTIDES OF HUMAN ERYTHROCYTE MEMBRANE, BIOCHEMISTRY 10: 2606 (1971). (10.1021/bi00789a030) / BIOCHEMISTRY (1971)
FISHER, K.A., ANALYSIS OF MEMBRANE HALVES - CHOLESTEROL, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 73: 173 (1976). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1976)
10.1126/science.1188378
GAHMBERG, C.G., SELECTIVE RADIOACTIVE LABELING OF CELL-SURFACE SIALOGLYCOPROTEINS BY PERIODATE-TRITIATED BOROHYDRIDE, JOURNAL OF BIOLOGICAL CHEMISTRY 252: 5888 (1977). (10.1016/S0021-9258(17)40107-4) / JOURNAL OF BIOLOGICAL CHEMISTRY (1977)
GRANT, CWM, GLYCOPHORIN IN LIPID BILAYERS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 71: 4653 (1974). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1974)
KOBYLKA, D, PROTEINS AND GLYCOPROTEINS OF ERYTHROCYTE-MEMBRANE, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 148: 475 (1972). (10.1016/0003-9861(72)90166-X) / ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS (1972)
10.1038/227680a0
LIU, S.C., SPONTANEOUS, REVERSIBLE PROTEIN CROSS-LINKING IN HUMAN ERYTHROCYTE-MEMBRANE - TEMPERATURE AND PH-DEPENDENCE, BIOCHEMISTRY 16: 4066 (1977). (10.1021/bi00637a020) / BIOCHEMISTRY (1977)
MUELLER, T.J., HETEROGENEITY OF SIALOGLYCOPROTEINS OF NORMAL HUMAN ERYTHROCYTE-MEMBRANE, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 72: 94 (1976). (10.1016/0006-291X(76)90965-7) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1976)
MUELLER, T.J., TRANSMEMBRANE PROTEINS IN PLASMA-MEMBRANE OF NORMAL HUMAN ERYTHROCYTES, JOURNAL OF BIOLOGICAL CHEMISTRY 249: 7568 (1974). (10.1016/S0021-9258(19)81275-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1974)
NAKAI, T, BIOCH BIOPHYS ACTA 422: 280 (1976). / BIOCH BIOPHYS ACTA (1976)
NICOLSON, G.L., ANIONIC SITES OF HUMAN ERYTHROCYTE-MEMBRANES .1. EFFECTS OF TRYPSIN, PHOSPHOLIPASE-C, AND PH ON TOPOGRAPHY OF BOUND POSITIVELY CHARGED COLLOIDAL PARTICLES, JOURNAL OF CELL BIOLOGY 57: 373 (1973). (10.1083/jcb.57.2.373) / JOURNAL OF CELL BIOLOGY (1973)
NICOLSON, G.L., ANIONIC SITES OF HUMAN ERYTHROCYTE-MEMBRANES .2. ANTISPECTRIN-INDUCED TRANSMEMBRANE AGGREGATION OF BINDING-SITES FOR POSITIVELY CHARGED COLLOIDAL PARTICLES, JOURNAL OF CELL BIOLOGY 59: 395 (1973). (10.1083/jcb.59.2.395) / JOURNAL OF CELL BIOLOGY (1973)
PHILLIPS, D.R., POSITION OF GLYCOPROTEIN POLYPEPTIDE CHAIN IN HUMAN ERYTHROCYTE MEMBRANE, FEBS LETTERS 18: 95 (1971). (10.1016/0014-5793(71)80416-7) / FEBS LETTERS (1971)
PINTODASILVA, P, FREEZE-ETCH LOCALIZATION OF CONCANAVALIN-A RECEPTORS TO MEMBRANE INTERCALATED PARTICLES OF HUMAN ERYTHROCYTE GHOST MEMBRANES, BIOCHIMICA ET BIOPHYSICA ACTA 363: 311 (1974). (10.1016/0005-2736(74)90071-6) / BIOCHIMICA ET BIOPHYSICA ACTA (1974)
PINTODASILVA, P, MEMBRANE SPLITTING IN FREEZE-ETCHING - COVALENTLY BOUND FERRITIN AS A MEMBRANE MARKER, JOURNAL OF CELL BIOLOGY 45: 598 (1970). (10.1083/jcb.45.3.598) / JOURNAL OF CELL BIOLOGY (1970)
PRICAM, C, INTRAMEMBRANOUS PARTICLE DISTRIBUTION IN HUMAN ERYTHROCYTES - EFFECTS OF LYSIS, GLUTARALDEHYDE, AND POLY-L-LYSINE, ANATOMICAL RECORD 189: 595 (1977). (10.1002/ar.1091890405) / ANATOMICAL RECORD (1977)
PRINGLE, J.R., METHOD CELL BIOL 12: 149 (1975). (10.1016/S0091-679X(08)60956-5) / METHOD CELL BIOL (1975)
SEGREST, J.P., ASSOCIATION OF MEMBRANE-PENETRATING POLYPEPTIDE SEGMENT OF HUMAN ERYTHROCYTE MN-GLYCOPROTEIN WITH PHOSPHOLIPID BILAYERS .1. FORMATION OF FREEZE-ETCH INTRAMEMBRANOUS PARTICLES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 71: 3294 (1974). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1974)
SILVERBERG, M, EFFECT OF CARBOXYMETHYLATING A SINGLE METHIONINE RESIDUE ON SUBUNIT INTERACTIONS OF GLYCOPHORIN-A, BIOCHEMISTRY 15: 1448 (1976). (10.1021/bi00652a015) / BIOCHEMISTRY (1976)
STECK, T.L., ORGANIZATION OF PROTEINS IN HUMAN RED BLOOD-CELL MEMBRANE - REVIEW, JOURNAL OF CELL BIOLOGY 62: 1 (1974). (10.1083/jcb.62.1.1) / JOURNAL OF CELL BIOLOGY (1974)
TILLACK, T.W., DEMONSTRATION OF OUTER SURFACE OF FREEZE-ETCHED RED BLOOD CELL MEMBRANES, JOURNAL OF CELL BIOLOGY 45: 649 (1970). (10.1083/jcb.45.3.649) / JOURNAL OF CELL BIOLOGY (1970)
TILLACK, T.W., STRUCTURE OF ERYTHROCYTE-MEMBRANES STUDIED BY FREEZE-ETCHING .2. LOCALIZATION OF RECEPTORS FOR PHYTOHEMAGGLUTININ AND INFLUENZA-VIRUS TO INTRAMEMBRANOUS PARTICLES, JOURNAL OF EXPERIMENTAL MEDICINE 135: 1209 (1972). (10.1084/jem.135.6.1209) / JOURNAL OF EXPERIMENTAL MEDICINE (1972)
TRICHE, T.J., LOCALIZATION OF BINDING-SITES FOR RICINUS-COMMUNIS, AGARICUS-BISPORUS AND WHEAT-GERM LECTINS ON HUMAN ERYTHROCYTE-MEMBRANES, BIOCHIMICA ET BIOPHYSICA ACTA 394: 540 (1975). (10.1016/0005-2736(75)90139-X) / BIOCHIMICA ET BIOPHYSICA ACTA (1975)
TUECH, J.K., HUMAN ERYTHROCYTE-MEMBRANE SIALOGLYCOPROTEINS - STUDY OF INTERCONVERSION, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 59: 352 (1974). (10.1016/S0006-291X(74)80214-7) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1974)
WOLOSIN, J.M., FUNCTIONAL CHARACTERIZATION OF ANION TRANSPORT-SYSTEM ISOLATED FROM HUMAN ERYTHROCYTE-MEMBRANES, JOURNAL OF BIOLOGICAL CHEMISTRY 252: 2419 (1977). (10.1016/S0021-9258(17)40570-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1977)
YU, J, RECONSTITUTION OF INTRAMEMBRANE PARTICLES IN RECOMBINANTS OF ERYTHROCYTE PROTEIN BAND-3 AND LIPID - EFFECTS OF SPECTRIN-ACTIN ASSOCIATION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 73: 3891 (1976). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1976)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 12:39 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 5:09 a.m.)
Indexed	1 year, 7 months ago (Jan. 14, 2024, 7:04 p.m.)
Issued	46 years, 4 months ago (March 30, 1979)
Published	46 years, 4 months ago (March 30, 1979)
Published Print	46 years, 4 months ago (March 30, 1979)

Funders 0

None

BibTeX

@article{Edwards_1979, title={Distribution of Transmembrane Polypeptides in Freeze Fracture}, volume={203}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.424755}, DOI={10.1126/science.424755}, number={4387}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Edwards, Harold H. and Mueller, Thomas J. and Morrison, Martin}, year={1979}, month=mar, pages={1343–1346} }

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