DOI: 10.1126/science.4001944. Filamentous Fusion Phage: Novel Expression Vectors That Display Cloned Antigens on the Virion Surface

Filamentous Fusion Phage: Novel Expression Vectors That Display Cloned Antigens on the Virion Surface

10.1126/science.4001944

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Foreign DNA fragments can be inserted into filamentous phage gene III to create a fusion protein with the foreign sequence in the middle. The fusion protein is incorporated into the virion, which retains infectivity and displays the foreign amino acids in immunologically accessible form. These "fusion phage" can be enriched more than 1000-fold over ordinary phage by affinity for antibody directed against the foreign sequence. Fusion phage may provide a simple way of cloning a gene when an antibody against the product of that gene is available.

Bibliography

Smith, G. P. (1985). Filamentous Fusion Phage: Novel Expression Vectors That Display Cloned Antigens on the Virion Surface. Science, 228(4705), 1315â1317.

Authors 1

George P. Smith (first)

References 18 Referenced 3,190

ARMSTRONG, J, EMBO JOURNAL 2: 1638 (1983). / EMBO JOURNAL (1983)
BECK, E, NUCLEOTIDE-SEQUENCE AND GENOME ORGANIZATION OF FILAMENTOUS BACTERIOPHAGES FL AND FD, GENE 16: 35 (1981). (10.1016/0378-1119(81)90059-7) / GENE (1981)
CRISSMAN, J.W., GENE-III PROTEIN OF FILAMENTOUS PHAGES - EVIDENCE FOR A CARBOXYL-TERMINAL DOMAIN WITH A ROLE IN MORPHOGENESIS, VIROLOGY 132: 445 (1984). (10.1016/0042-6822(84)90049-7) / VIROLOGY (1984)
DAY, L.A., CONFORMATIONS OF SINGLE-STRANDED DNA AND COAT PROTEIN IN FD BACTERIOPHAGE AS REVEALED BY ULTRAVIOLET ABSORPTION SPECTROSCOPY, JOURNAL OF MOLECULAR BIOLOGY 39: 265 (1969). (10.1016/0022-2836(69)90316-7) / JOURNAL OF MOLECULAR BIOLOGY (1969)
GRANT, R.A., STRUCTURE OF THE FILAMENTOUS BACTERIOPHAGE-FL - LOCATION OF THE MINOR COAT PROTEIN-A, PROTEIN-C AND PROTEIN-D, JOURNAL OF BIOLOGICAL CHEMISTRY 256: 539 (1981). (10.1016/S0021-9258(19)70172-0) / JOURNAL OF BIOLOGICAL CHEMISTRY (1981)
GRAY, C.W., ADSORPTION COMPLEX OF FILAMENTOUS FD VIRUS, JOURNAL OF MOLECULAR BIOLOGY 146: 621 (1981). (10.1016/0022-2836(81)90050-4) / JOURNAL OF MOLECULAR BIOLOGY (1981)
Hackett P. B. An Introduction to Recombinant DNA Techniques (1984).
HILL, D.F., NUCLEOTIDE-SEQUENCE OF BACTERIOPHAGE-F1 DNA, JOURNAL OF VIROLOGY 44: 32 (1982). (10.1128/jvi.44.1.32-46.1982) / JOURNAL OF VIROLOGY (1982)
LYONS, L.B., GENETIC MAP OF FILAMENTOUS BACTERIOPHAGE F1, VIROLOGY 49: 45 (1972). (10.1016/S0042-6822(72)80006-0) / VIROLOGY (1972)
Maniatis T. Molecular Cloning: A Laboratory Manual (1982).
MESSING, J, A NEW PAIR OF M13 VECTORS FOR SELECTING EITHER DNA STRAND OF DOUBLE-DIGEST RESTRICTION FRAGMENTS, GENE 19: 269 (1982). (10.1016/0378-1119(82)90016-6) / GENE (1982)
MODRICH, P, ECORI ENDONUCLEASE - PHYSICAL AND CATALYTIC PROPERTIES OF HOMOGENEOUS ENZYME, JOURNAL OF BIOLOGICAL CHEMISTRY 251: 5866 (1976). (10.1016/S0021-9258(17)33032-6) / JOURNAL OF BIOLOGICAL CHEMISTRY (1976)
NELSON, F.K., FILAMENTOUS PHAGE DNA CLONING VECTORS - A NON-INFECTIVE MUTANT WITH A NON-POLAR DELETION IN GENE-III, VIROLOGY 108: 338 (1981). (10.1016/0042-6822(81)90442-6) / VIROLOGY (1981)
NEWMAN, A.K., DNA-SEQUENCES OF STRUCTURAL GENES FOR ECO RI DNA RESTRICTION AND MODIFICATION ENZYMES, JOURNAL OF BIOLOGICAL CHEMISTRY 256: 2131 (1981). (10.1016/S0021-9258(19)69750-4) / JOURNAL OF BIOLOGICAL CHEMISTRY (1981)
PRATT, D, CONDITIONAL LETHAL MUTANTS OF SMALL FILAMENTOUS COLIPHAGE M13 .2. 2 GENES FOR COAT PROTEINS, VIROLOGY 39: 42 (1969). (10.1016/0042-6822(69)90346-8) / VIROLOGY (1969)
RUBIN, R.A., ECORI METHYLASE - PHYSICAL AND CATALYTIC PROPERTIES OF HOMOGENEOUS ENZYME, JOURNAL OF BIOLOGICAL CHEMISTRY 252: 7265 (1977). (10.1016/S0021-9258(19)66964-4) / JOURNAL OF BIOLOGICAL CHEMISTRY (1977)
SALIVAR, W.O., SOME PHYSICAL-CHEMICAL + BIOLOGICAL PROPERTIES OF ROD-SHAPED COLIPHAGE M13, VIROLOGY 24: 359 (1964). (10.1016/0042-6822(64)90173-4) / VIROLOGY (1964)
Young, R. A., Proceedings of the National Academy of Sciences of the United States of America 80: 1194 (1983). / Proceedings of the National Academy of Sciences of the United States of America (1983)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 3:42 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 4:18 a.m.)
Indexed	6 hours, 30 minutes ago (Aug. 27, 2025, 11:39 a.m.)
Issued	40 years, 2 months ago (June 14, 1985)
Published	40 years, 2 months ago (June 14, 1985)
Published Print	40 years, 2 months ago (June 14, 1985)

Funders 0

None

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