Transformation of Human Bronchial Epithelial Cells Transfected by Harvey ras Oncogene
10.1126/science.3975607
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Transfection of normal human bronchial epithelial (NHBE) cells with a plasmid carrying the ras oncogene of Harvey murine sarcoma virus (v-Ha ras ) changed the growth requirements, terminal differentiation, and tumorigenicity of the recipient cells. One of the cell lines isolated after transfection (TBE-1) was studied extensively and shown to contain v-Ha ras DNA. Total cellular RNA from TBE-1 cells hybridized to v-Ha ras structural gene fragment probes five to eight times more than RNA from parental NHBE cells. The TBE-1 cells expressed phosphorylated v-Ha ras polypeptide p21, showed a reduced requirement for growth-factor supplements, and became aneuploid as an early cellular response to v-Ha ras expression. As the transfectants acquire an indefinite life-span and anchorage independence they became transplantable tumor cells and showed many phenotypic changes suggesting a pleiotropic mechanism for the role of Ha ras in human carcinogenesis.

Bibliography

Yoakum, G. H., Lechner, J. F., Gabrielson, E. W., Korba, B. E., Malan-Shibley, L., Willey, J. C., Valerio, M. G., Shamsuddin, A. M., Trump, B. F., & Harris, C. C. (1985). Transformation of Human Bronchial Epithelial Cells Transfected by Harvey ras Oncogene. Science, 227(4691), 1174–1179.

Authors 10
  1. George H. Yoakum (first)
  2. John F. Lechner (additional)
  3. Edward W. Gabrielson (additional)
  4. Brent E. Korba (additional)
  5. Louise Malan-Shibley (additional)
  6. James C. Willey (additional)
  7. Marion G. Valerio (additional)
  8. Abulkalam M. Shamsuddin (additional)
  9. Benjamin F. Trump (additional)
  10. Curtis C. Harris (additional)
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Dates
Type When
Created 18 years, 10 months ago (Oct. 5, 2006, 3:42 p.m.)
Deposited 1 year, 7 months ago (Jan. 14, 2024, 4:14 a.m.)
Indexed 11 months, 3 weeks ago (Sept. 6, 2024, 7:17 p.m.)
Issued 40 years, 5 months ago (March 8, 1985)
Published 40 years, 5 months ago (March 8, 1985)
Published Print 40 years, 5 months ago (March 8, 1985)
Funders 0

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@article{Yoakum_1985, title={Transformation of Human Bronchial Epithelial Cells Transfected by Harvey ras Oncogene}, volume={227}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3975607}, DOI={10.1126/science.3975607}, number={4691}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Yoakum, George H. and Lechner, John F. and Gabrielson, Edward W. and Korba, Brent E. and Malan-Shibley, Louise and Willey, James C. and Valerio, Marion G. and Shamsuddin, Abulkalam M. and Trump, Benjamin F. and Harris, Curtis C.}, year={1985}, month=mar, pages={1174–1179} }