DOI: 10.1126/science.3952500. Electrophoretic Separations of Large DNA Molecules by Periodic Inversion of the Electric Field

Electrophoretic Separations of Large DNA Molecules by Periodic Inversion of the Electric Field

10.1126/science.3952500

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

In gel electrophoresis, nucleic acids and protein-detergent complexes larger than a threshold size all migrate at the same rate. For DNA molecules, this effect can be overcome by the simple procedure of periodically inverting the electric field. Tuning the frequency of the field inversions from 10 to 0.01 hertz, makes it possible to resolve selectively DNA's in the size range 15 to >700 kilobase pairs.

Bibliography

Carle, G. F., Frank, M., & Olson, M. V. (1986). Electrophoretic Separations of Large DNA Molecules by Periodic Inversion of the Electric Field. Science, 232(4746), 65â68.

Authors 3

Georges F. Carle (first)
Mark Frank (additional)
Maynard V. Olson (additional)

References 16 Referenced 654

ALLEN R.C. GEL ELECTROPHORESIS (1984).
10.1093/nar/12.14.5647
CARLE, G.F., AN ELECTROPHORETIC KARYOTYPE FOR YEAST, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 82: 3756 (1985). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1985)
DEGENNES, P.G., REPTATION OF A POLYMER CHAIN IN PRESENCE OF FIXED OBSTACLES, JOURNAL OF CHEMICAL PHYSICS 55: 572 (1971). (10.1063/1.1675789) / JOURNAL OF CHEMICAL PHYSICS (1971)
FANGMAN, W.L., SEPARATION OF VERY LARGE DNA-MOLECULES BY GEL-ELECTROPHORESIS, NUCLEIC ACIDS RESEARCH 5: 653 (1978). (10.1093/nar/5.3.653) / NUCLEIC ACIDS RESEARCH (1978)
JAMIL, T, DEPENDENCE OF THE ELECTROPHORETIC MOBILITY OF DNA IN GELS ON FIELD INTERMITTENCY, JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS 2: 963 (1985). (10.1080/07391102.1985.10507612) / JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS (1985)
KEMP, D.J., SIZE VARIATION IN CHROMOSOMES FROM INDEPENDENT CULTURED ISOLATES OF PLASMODIUM-FALCIPARUM, NATURE 315: 347 (1985). (10.1038/315347a0) / NATURE (1985)
LERMAN, L.S., WHY DOES THE ELECTROPHORETIC MOBILITY OF DNA IN GELS VARY WITH THE LENGTH OF THE MOLECULE, BIOPOLYMERS 21: 995 (1982). (10.1002/bip.360210511) / BIOPOLYMERS (1982)
LUMPKIN, O.J., THEORY OF GEL-ELECTROPHORESIS OF DNA, BIOPOLYMERS 24: 1573 (1985). (10.1002/bip.360240812) / BIOPOLYMERS (1985)
Maniatis, T., Molecular Cloning: A Laboratory Manual: 153 (1982). / Molecular Cloning: A Laboratory Manual (1982)
RICKWOOD D GEL ELECTROPHORESIS (1982).
10.1016/0092-8674(84)90301-5
Schwartz, D. C., Cold Spring Harbor Symposia on Quantitative Biology 47: 189 (1983). (10.1101/SQB.1983.047.01.024) / Cold Spring Harbor Symposia on Quantitative Biology (1983)
SERWER, P, ELECTROPHORESIS OF DUPLEX DEOXYRIBONUCLEIC-ACID IN MULTIPLE-CONCENTRATION AGAROSE GELS - FRACTIONATION OF MOLECULES WITH MOLECULAR-WEIGHTS BETWEEN 2 X 106 AND 110 X 106, BIOCHEMISTRY 19: 3001 (1980). (10.1021/bi00554a026) / BIOCHEMISTRY (1980)
VANDERPLOEG, LHT, ANTIGENIC VARIATION IN TRYPANOSOMA BRUCEI ANALYZED BY ELECTROPHORETIC SEPARATION OF CHROMOSOME-SIZED DNA-MOLECULES, CELL 37: 77 (1984). (10.1016/0092-8674(84)90302-7) / CELL (1984)
VANDERPLOEG, LHT, CHROMOSOME-SIZED DNA-MOLECULES OF PLASMODIUM-FALCIPARUM, SCIENCE 229: 658 (1985). (10.1126/science.3895435) / SCIENCE (1985)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 4:28 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 4:27 a.m.)
Indexed	3 weeks, 1 day ago (Aug. 5, 2025, 8:58 a.m.)
Issued	39 years, 4 months ago (April 4, 1986)
Published	39 years, 4 months ago (April 4, 1986)
Published Print	39 years, 4 months ago (April 4, 1986)

Funders 0

None

BibTeX

@article{Carle_1986, title={Electrophoretic Separations of Large DNA Molecules by Periodic Inversion of the Electric Field}, volume={232}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3952500}, DOI={10.1126/science.3952500}, number={4746}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Carle, Georges F. and Frank, Mark and Olson, Maynard V.}, year={1986}, month=apr, pages={65–68} }

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