DOI: 10.1126/science.3906896. Systematic Variation in DNA Length Yields Highly Ordered Repressor-Operator Cocrystals

Systematic Variation in DNA Length Yields Highly Ordered Repressor-Operator Cocrystals

10.1126/science.3906896

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Crystals have been grown that contain the operator-binding domain of the λ repressor and the λ operator site O L 1. Crystallization conditions were tested with a set of DNA fragments, ranging in length from 17 to 23 base pairs. The best crystals were grown with a 20-base pair DNA fragment. These crystals have space-group symmetry P2 1 , with unit cell dimensions a = 37.1 Å, b = 68.8 Å, c = 56.8 Å, and a β angle of 91.5°. They diffracted to at least 2.5 Å resolution. High resolution data from these crystals should allow the direct determination of how a repressor recognizes its operator site.

Bibliography

Jordan, S. R., Whitcombe, T. V., Berg, J. M., & Pabo, C. O. (1985). Systematic Variation in DNA Length Yields Highly Ordered Repressor-Operator Cocrystals. Science, 230(4732), 1383â1385.

Authors 4

Steven R. Jordan (first)
Timothy V. Whitcombe (additional)
Jeremy M. Berg (additional)
Carl O. Pabo (additional)

References 27 Referenced 47

Anderson, J., Proceedings of the National Academy of Sciences of the United States of America 81: 1307 (1984). / Proceedings of the National Academy of Sciences of the United States of America (1984)
ANDERSON, J.E., A PHAGE REPRESSOR OPERATOR COMPLEX AT 7-A RESOLUTION, NATURE 316: 596 (1985). (10.1038/316596a0) / NATURE (1985)
ANDERSON, W.F., STRUCTURE OF THE CRO REPRESSOR FROM BACTERIOPHAGE-LAMBDA AND ITS INTERACTION WITH DNA, NATURE 290: 754 (1981). (10.1038/290754a0) / NATURE (1981)
ARNOTT, S, OPTIMIZED PARAMETERS FOR A-DNA AND B-DNA, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 47: 1504 (1972). (10.1016/0006-291X(72)90243-4) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1972)
BEAUCAGE, S.L., DEOXYNUCLEOSIDE PHOSPHORAMIDITES - A NEW CLASS OF KEY INTERMEDIATES FOR DEOXYPOLYNUCLEOTIDE SYNTHESIS, TETRAHEDRON LETTERS 22: 1859 (1981). (10.1016/S0040-4039(01)90461-7) / TETRAHEDRON LETTERS (1981)
DICKERSON, R.E., STRUCTURE OF A B-DNA DODECAMER .2. INFLUENCE OF BASE SEQUENCE ON HELIX STRUCTURE, JOURNAL OF MOLECULAR BIOLOGY 149: 761 (1981). (10.1016/0022-2836(81)90357-0) / JOURNAL OF MOLECULAR BIOLOGY (1981)
10.1038/309327a0
10.1093/nar/9.23.6505
Hecht, M. H., Proceedings of the National Academy of Sciences of the United States of America 80: 2676 (1983). / Proceedings of the National Academy of Sciences of the United States of America (1983)
JOHNSON A.D. THESIS HARVARD U (1980).
Lewis, M., Cold Spring Harbor Symposia on Quantitative Biology 47: 435 (1983). (10.1101/SQB.1983.047.01.051) / Cold Spring Harbor Symposia on Quantitative Biology (1983)
Maniatis T. Molecular Cloning: A Laboratory Manual (1982).
10.1016/0022-2836(68)90205-2
10.1038/290744a0
McPherson A. Preparation and Analysis of Protein Crystals (1982).
Nelson, H. C. M., Cold Spring Harbor Symposia on Quantitative Biology 47: 441 (1983). (10.1101/SQB.1983.047.01.052) / Cold Spring Harbor Symposia on Quantitative Biology (1983)
OHLENDORF, D.H., THE MOLECULAR-BASIS OF DNA PROTEIN RECOGNITION INFERRED FROM THE STRUCTURE OF CRO REPRESSOR, NATURE 298: 718 (1982). (10.1038/298718a0) / NATURE (1982)
10.1146/annurev.bi.53.070184.001453
10.1038/298443a0
PABO, C.O., LAMBDA REPRESSOR CONTAINS 2 DOMAINS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 76: 1608 (1979). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1979)
PABO C.O. THESIS HARVARD U (1980).
SAUER, R.T., HOMOLOGY AMONG DNA-BINDING PROTEINS SUGGESTS USE OF A CONSERVED SUPER-SECONDARY STRUCTURE, NATURE 298: 447 (1982). (10.1038/298447a0) / NATURE (1982)
SCHEVITZ, R.W., THE 3-DIMENSIONAL STRUCTURE OF TRP REPRESSOR, NATURE 317: 782 (1985). (10.1038/317782a0) / NATURE (1985)
Steitz, T. A., Proceedings of the National Academy of Sciences of the United States of America 79: 3097 (1982). / Proceedings of the National Academy of Sciences of the United States of America (1982)
WANG, AHJ, MOLECULAR-STRUCTURE OF A LEFT-HANDED DOUBLE HELICAL DNA FRAGMENT AT ATOMIC RESOLUTION, NATURE 282: 680 (1979). (10.1038/282680a0) / NATURE (1979)
WANG, J.C., HELICAL REPEAT OF DNA IN SOLUTION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 76: 200 (1979). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1979)
Weber, I. T., Proceedings of the National Academy of Sciences of the United States of America 81: 3973 (1984). / Proceedings of the National Academy of Sciences of the United States of America (1984)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 3:42 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 4:33 a.m.)
Indexed	21 hours, 2 minutes ago (Sept. 4, 2025, 9:32 a.m.)
Issued	39 years, 8 months ago (Dec. 20, 1985)
Published	39 years, 8 months ago (Dec. 20, 1985)
Published Print	39 years, 8 months ago (Dec. 20, 1985)

Funders 0

None

BibTeX

@article{Jordan_1985, title={Systematic Variation in DNA Length Yields Highly Ordered Repressor-Operator Cocrystals}, volume={230}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3906896}, DOI={10.1126/science.3906896}, number={4732}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Jordan, Steven R. and Whitcombe, Timothy V. and Berg, Jeremy M. and Pabo, Carl O.}, year={1985}, month=dec, pages={1383–1385} }

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