Abstract
A 2.7 angstrom resolution x-ray diffraction analysis of a trypsin-modified form of the Escherichia coli elongation factor Tu reveals that the GDP-binding domain has a structure similar to that of other nucleotide-binding proteins. The GDP ligand is located at the COOH-terminal end of the β sheet and is linked to the protein via a Mg 2+ ion salt bridge. The location of the guanine ring is unusual; the purine ring is located on the outer edge of the domain, not deep within a hydrophobic pocket. The amino acids from Pro 10 to Arg 44 and from Gly 59 to Glu 190 have been assigned to the electron density with computer graphic techniques, and the resulting model is consistent with all known biochemical data. An analysis of the structure reveals that four regions of the amino acid sequence that are homologous with the family of ras oncogene proteins, termed p21, are located in the vicinity of the GDP-binding site, and most of the invariant amino acids shared by the proteins interact directly with the GDP ligand.
References
23
Referenced
470
- ARAI, K, PRIMARY STRUCTURE OF ELONGATION-FACTOR TU FROM ESCHERICHIA-COLI, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 77: 1326 (1980). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1980)
- BHAT, T.N., ACTA CRYST A 38: 22 (1982). / ACTA CRYST A (1982)
- BLUNDELL, T.L., PROTEIN CRYSTALLOGRA: 373 (1977). / PROTEIN CRYSTALLOGRA (1977)
- CLARK, BFC, CELL FUNCTION DIFF C: 59 (1982). / CELL FUNCTION DIFF C (1982)
- CLARK, R, ANTIBODIES SPECIFIC FOR AMINO-ACID 12 OF THE RAS ONCOGENE PRODUCT INHIBIT GTP BINDING, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 82: 5280 (1985). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1985)
- HALLIDAY, K.R., REGIONAL HOMOLOGY IN GTP-BINDING PROTO-ONCOGENE PRODUCTS AND ELONGATION-FACTORS, JOURNAL OF CYCLIC NUCLEOTIDE AND PROTEIN PHOSPHORYLATION RESEARCH 9: 435 (1984). / JOURNAL OF CYCLIC NUCLEOTIDE AND PROTEIN PHOSPHORYLATION RESEARCH (1984)
-
HAMLIN, R, CHARACTERISTICS OF A FLAT MULTIWIRE AREA DETECTOR FOR PROTEIN CRYSTALLOGRAPHY, JOURNAL OF APPLIED CRYSTALLOGRAPHY 14: 85 (1981).
(
10.1107/S0021889881008856
) / JOURNAL OF APPLIED CRYSTALLOGRAPHY (1981) -
HOL, WGJ, ALPHA-HELIX DIPOLE AND PROPERTIES OF PROTEINS, NATURE 273: 443 (1978).
(
10.1038/273443a0
) / NATURE (1978) -
JURNAK, F, BIOCHEMICAL AND STRUCTURAL STUDIES OF THE TETRAGONAL CRYSTALLINE MODIFICATION OF THE ESCHERICHIA-COLI ELONGATION FACTOR-TU, JOURNAL OF BIOLOGICAL CHEMISTRY 255: 6751 (1980).
(
10.1016/S0021-9258(18)43636-8
) / JOURNAL OF BIOLOGICAL CHEMISTRY (1980) -
Jurnak, F., Journal of Molecular Biology 185: 215 (1985).
(
10.1016/0022-2836(85)90194-9
) / Journal of Molecular Biology (1985) -
JURNAK, F, PRELIMINARY X-RAY-DIFFRACTION DATA FOR TETRAGONAL CRYSTALS OF TRYPSINIZED ESCHERICHIA-COLI ELONGATION-FACTOR, JOURNAL OF MOLECULAR BIOLOGY 115: 103 (1977).
(
10.1016/0022-2836(77)90250-9
) / JOURNAL OF MOLECULAR BIOLOGY (1977) -
LAURSEN, R.A., AMINO-ACID SEQUENCE OF ELONGATION FACTOR-TU CHARACTERIZATION AND ALIGNMENT OF CYANOGEN-BROMIDE FRAGMENTS AND LOCATION OF CYSTEINE RESIDUES, FEBS LETTERS 80: 103 (1977).
(
10.1016/0014-5793(77)80416-X
) / FEBS LETTERS (1977) -
LEBERMAN, R, HOMOLOGIES IN THE PRIMARY STRUCTURE OF GTP-BINDING PROTEINS - THE NUCLEOTIDE-BINDING SITE OF EF-TU AND P21, EMBO JOURNAL 3: 339 (1984).
(
10.1002/j.1460-2075.1984.tb01808.x
) / EMBO JOURNAL (1984) 10.1126/science.3856323
- MILLER, D.L., MOL MECHANISMS PROTE: 323 (1977). / MOL MECHANISMS PROTE (1977)
-
MORIKAWA, K, HIGH-RESOLUTION X-RAY CRYSTALLOGRAPHIC ANALYSIS OF A MODIFIED FORM OF ELONGATION-FACTOR TU - GUANOSINE DIPHOSPHATE COMPLEX, JOURNAL OF MOLECULAR BIOLOGY 125: 325 (1978).
(
10.1016/0022-2836(78)90406-0
) / JOURNAL OF MOLECULAR BIOLOGY (1978) - SCOLNICK, E.M., GUANINE NUCLEOTIDE-BINDING ACTIVITY AS AN ASSAY FOR SRC PROTEIN OF RAT-DERIVED MURINE SARCOMA-VIRUSES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 76: 5355 (1979). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1979)
-
SMITH, S.B., THE PURIFICATION AND CHARACTERIZATION OF DELTA-5-3-KETOSTEROID ISOMERASE FROM PSEUDOMONAS-PUTIDA, A CYSTEINE-CONTAINING ISOMERASE, JOURNAL OF BIOLOGICAL CHEMISTRY 255: 2678 (1980).
(
10.1016/S0021-9258(19)85789-7
) / JOURNAL OF BIOLOGICAL CHEMISTRY (1980) -
TABIN, C.J., MECHANISM OF ACTIVATION OF A HUMAN ONCOGENE, NATURE 300: 143 (1982).
(
10.1038/300143a0
) / NATURE (1982) -
TERWILLIGER, T.C., UNBIASED 3-DIMENSIONAL REFINEMENT OF HEAVY-ATOM PARAMETERS BY CORRELATION OF ORIGIN-REMOVED PATTERSON FUNCTIONS, ACTA CRYSTALLOGRAPHICA SECTION A 39: 813 (1983).
(
10.1107/S0108767383001592
) / ACTA CRYSTALLOGRAPHICA SECTION A (1983) - WANG, B.C., A METHOD FOR RESOLVING PHASE AMBIGUITY IN SIR OR SINGLE-WAVELENGTH ANOMALOUS SCATTERING DATA, ACTA CRYSTALLOGRAPHICA SECTION A 40: C12 (1984). / ACTA CRYSTALLOGRAPHICA SECTION A (1984)
-
WITTINGHOFER, A, COMPOSITION AND PROPERTIES OF TRYPSIN-CLEAVED ELONGATION FACTOR-TU, EUROPEAN JOURNAL OF BIOCHEMISTRY 108: 423 (1980).
(
10.1111/j.1432-1033.1980.tb04738.x
) / EUROPEAN JOURNAL OF BIOCHEMISTRY (1980) -
WITTINGHOFER, A, THE STRUCTURE OF THE EF-TU.GDP.ME2+ COMPLEX, EUROPEAN JOURNAL OF BIOCHEMISTRY 124: 109 (1982).
(
10.1111/j.1432-1033.1982.tb05912.x
) / EUROPEAN JOURNAL OF BIOCHEMISTRY (1982)
Dates
Type | When |
---|---|
Created | 18 years, 10 months ago (Oct. 5, 2006, 3:42 p.m.) |
Deposited | 1 year, 7 months ago (Jan. 14, 2024, 4:26 a.m.) |
Indexed | 4 months ago (April 19, 2025, 12:10 a.m.) |
Issued | 39 years, 10 months ago (Oct. 4, 1985) |
Published | 39 years, 10 months ago (Oct. 4, 1985) |
Published Print | 39 years, 10 months ago (Oct. 4, 1985) |
@article{Jurnak_1985, title={Structure of the GDP Domain of EF-Tu and Location of the Amino Acids Homologous to ras Oncogene Proteins}, volume={230}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3898365}, DOI={10.1126/science.3898365}, number={4721}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Jurnak, Frances}, year={1985}, month=oct, pages={32–36} }