DOI: 10.1126/science.3883490. Receptor-Mediated Transport of Insulin Across Endothelial Cells

Receptor-Mediated Transport of Insulin Across Endothelial Cells

10.1126/science.3883490

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Hormones such as insulin are transported from the interior to the exterior of blood vessels. Whether endothelial cells, which line the inner walls of blood vessels have a role in this transport of hormones is not clear, but it is known that endothelial cells can internalize and release insulin rapidly with little degradation. The transport of iodine-125-labeled insulin was measured directly through the use of dual chambers separated by a horizontal monolayer of cultured bovine aortic endothelial cells. In this setting, endothelial cells took up and released the labeled insulin, thereby transporting it across the cells. The transport of insulin across the endothelial cells was temperature sensitive and was inhibited by unlabeled insulin and by antibody to insulin receptor in proportion to the ability of these substances to inhibit insulin binding to its receptor. More than 80 percent of the transported insulin was intact. These data suggest that insulin is rapidly transported across endothelial cells by a receptor-mediated process.

Bibliography

King, G. L., & Johnson, S. M. (1985). Receptor-Mediated Transport of Insulin Across Endothelial Cells. Science, 227(4694), 1583â1586.

Authors 2

George L. King (first)
Sandra M. Johnson (additional)

References 20 Referenced 344

BAR, R.S., RECEPTORS FOR MULTIPLICATION-STIMULATING ACTIVITY ON HUMAN ARTERIAL AND VENOUS ENDOTHELIAL-CELLS, JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM 52: 814 (1981). (10.1210/jcem-52-4-814) / JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM (1981)
CARSON, M. P., IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION 19: 833 (1983). / IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION (1983)
DERNOVSEK, K.D., RAPID-TRANSPORT OF BIOLOGICALLY INTACT INSULIN THROUGH CULTURED ENDOTHELIAL-CELLS, JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM 58: 761 (1984). (10.1210/jcem-58-4-761) / JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM (1984)
GLIEMANN, J, BIOLOGICAL POTENCY AND BINDING-AFFINITY OF MONOIODOINSULIN WITH IODINE IN TYROSINE-A14 OR TYROSINE-A19, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 87: 1183 (1979). (10.1016/S0006-291X(79)80032-7) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1979)
GORDEN, P, INTERNALIZATION OF POLYPEPTIDE HORMONES - MECHANISM, INTRACELLULAR-LOCALIZATION AND SIGNIFICANCE, DIABETOLOGIA 18: 263 (1980). / DIABETOLOGIA (1980)
GRIGORESCU, F, INSULIN BINDING AND INSULIN-DEPENDENT PHOSPHORYLATION OF THE INSULIN-RECEPTOR SOLUBILIZED FROM HUMAN-ERYTHROCYTES, JOURNAL OF BIOLOGICAL CHEMISTRY 258: 13708 (1983). (10.1016/S0021-9258(17)43975-5) / JOURNAL OF BIOLOGICAL CHEMISTRY (1983)
JIALAL, I, PROCESSING OF INSULIN BY BOVINE ENDOTHELIAL-CELLS IN CULTURE - INTERNALIZATION WITHOUT DEGRADATION, DIABETES 33: 794 (1984). (10.2337/diab.33.8.794) / DIABETES (1984)
KAHN, C.R., EFFECTS OF AUTOANTIBODIES TO INSULIN RECEPTOR ON ISOLATED ADIPOCYTES - STUDIES OF INSULIN BINDING AND INSULIN ACTION, JOURNAL OF CLINICAL INVESTIGATION 60: 1094 (1977). (10.1172/JCI108861) / JOURNAL OF CLINICAL INVESTIGATION (1977)
KAHN, C.R., DIRECT DEMONSTRATION THAT RECEPTOR CROSSLINKING OR AGGREGATION IS IMPORTANT IN INSULIN ACTION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 75: 4209 (1978). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1978)
KAISER, N, BINDING, INTERNALIZATION, AND DEGRADATION OF INSULIN IN VASCULAR ENDOTHELIAL-CELLS, DIABETES 31: 1077 (1982). (10.2337/diacare.31.12.1077) / DIABETES (1982)
KING, G.L., DIRECT DEMONSTRATION OF SEPARATE RECEPTORS FOR GROWTH AND METABOLIC-ACTIVITIES OF INSULIN AND MULTIPLICATION-STIMULATING ACTIVITY (AN INSULIN-LIKE GROWTH-FACTOR) USING ANTIBODIES TO THE INSULIN-RECEPTOR, JOURNAL OF CLINICAL INVESTIGATION 66: 130 (1980). (10.1172/JCI109826) / JOURNAL OF CLINICAL INVESTIGATION (1980)
KING, G.L., DIFFERENTIAL RESPONSIVENESS TO INSULIN OF ENDOTHELIAL AND SUPPORT CELLS FROM MICROVESSELS AND MACROVESSELS, JOURNAL OF CLINICAL INVESTIGATION 71: 974 (1983). (10.1172/JCI110852) / JOURNAL OF CLINICAL INVESTIGATION (1983)
PALADE, G.E., ACTA PHYSIOLOGICA SCANDINAVICA 463: 11 (1979). / ACTA PHYSIOLOGICA SCANDINAVICA (1979)
POSNER, B.I., RECENT PROGRESS IN HORMONE RESEARCH 37: 539 (1981). / RECENT PROGRESS IN HORMONE RESEARCH (1981)
RENKIN, E.M., ACTA PHYSIOLOGICA SCANDINAVICA 463: 81 (1979). / ACTA PHYSIOLOGICA SCANDINAVICA (1979)
ROSIO, E.A., DIABETES CARE 5: 158 (1982). (10.2337/diacare.5.3.158) / DIABETES CARE (1982)
ROSIO, E.A., JOURNAL OF CLINICAL INVESTIGATION 46: 903 (1967). (10.1172/JCI105596) / JOURNAL OF CLINICAL INVESTIGATION (1967)
SCHWARTZ, S. M., IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION 14: 966 (1978). / IN VITRO-JOURNAL OF THE TISSUE CULTURE ASSOCIATION (1978)
SIMIONESCU, N, PERMEABILITY OF MUSCLE CAPILLARIES TO SMALL HEME-PEPTIDES - EVIDENCE FOR EXISTENCE OF PATENT TRANSENDOTHELIAL CHANNELS, JOURNAL OF CELL BIOLOGY 64: 586 (1975). (10.1083/jcb.64.3.586) / JOURNAL OF CELL BIOLOGY (1975)
SONKSEN, P.H., DISTRIBUTION AND BINDING OF INSULIN IN DOG HINDLIMB, AMERICAN JOURNAL OF PHYSIOLOGY 221: 1672 (1971). (10.1152/ajplegacy.1971.221.6.1672) / AMERICAN JOURNAL OF PHYSIOLOGY (1971)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 3:42 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 4:14 a.m.)
Indexed	21 hours, 13 minutes ago (Aug. 31, 2025, 6:17 a.m.)
Issued	40 years, 5 months ago (March 29, 1985)
Published	40 years, 5 months ago (March 29, 1985)
Published Print	40 years, 5 months ago (March 29, 1985)

Funders 0

None

BibTeX

@article{King_1985, title={Receptor-Mediated Transport of Insulin Across Endothelial Cells}, volume={227}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3883490}, DOI={10.1126/science.3883490}, number={4694}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={King, George L. and Johnson, Sandra M.}, year={1985}, month=mar, pages={1583–1586} }

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