DOI: 10.1126/science.3856323. Sequence of the Alpha Subunit of Photoreceptor G Protein: Homologies Between Transducin, <i>ras</i> , and Elongation Factors

Sequence of the Alpha Subunit of Photoreceptor G Protein: Homologies Between Transducin, ras , and Elongation Factors

10.1126/science.3856323

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A bovine retinal complementary DNA clone encoding the α subunit of transducin (T α ) was isolated with the use of synthetic oligodeoxynucleotides as probes, and the complete nucleotide sequence of the insert was determined. The predicted protein sequence of 354 amino acids includes the known sequences of four tryptic peptides and sequences adjacent to the residues that undergo adenosine diphosphate ribosylation by cholera toxin and pertussis toxin. On the basis of homologies to other proteins, such as the elongation factors of protein synthesis and the ras oncogene proteins, regions are identified that are predicted to be acylated and involved in guanine nucleotide binding and hydrolysis. Amino acid sequence similarity between T α and ras is confined to these regions of the molecules.

Bibliography

Lochrie, M. A., Hurley, J. B., & Simon, M. I. (1985). Sequence of the Alpha Subunit of Photoreceptor G Protein: Homologies Between Transducin, ras , and Elongation Factors. Science, 228(4695), 96â99.

Authors 3

Michael A. Lochrie (first)
James B. Hurley (additional)
Melvin I. Simon (additional)

References 43 Referenced 198

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 3:42 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 5:01 a.m.)
Indexed	4 months, 2 weeks ago (April 8, 2025, 10:19 p.m.)
Issued	40 years, 4 months ago (April 5, 1985)
Published	40 years, 4 months ago (April 5, 1985)
Published Print	40 years, 4 months ago (April 5, 1985)

Funders 0

None

BibTeX

@article{Lochrie_1985, title={Sequence of the Alpha Subunit of Photoreceptor G Protein: Homologies Between Transducin, ras , and Elongation Factors}, volume={228}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3856323}, DOI={10.1126/science.3856323}, number={4695}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Lochrie, Michael A. and Hurley, James B. and Simon, Melvin I.}, year={1985}, month=apr, pages={96–99} }

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