DOI: 10.1126/science.3839937. Molecular Cloning of Complementary DNA for the Alpha Subunit of the G Protein that Stimulates Adenylate Cyclase

Molecular Cloning of Complementary DNA for the Alpha Subunit of the G Protein that Stimulates Adenylate Cyclase

10.1126/science.3839937

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A complementary DNA clone encoding the α subunit of the adenylate cyclase stimulatory G protein (G s ) was isolated and identified. A bovine brain complementary DNA library was screened with an oligonucleotide probe derived from amino acid sequence common to known G proteins. The only clone that was obtained with this probe has a complementary DNA insert of approximately 1670 base pairs. An antibody to a peptide synthesized according to deduced amino acid sequence reacts specifically with the α subunit of G s . In addition, RNA that hybridizes with probes made from the clone is detected in wild-type S49 cells; however, cyc - S49 cells, which are deficient in G sα activity, are devoid of this messenger RNA.

Bibliography

Harris, B. A., Robishaw, J. D., Mumby, S. M., & Gilman, A. G. (1985). Molecular Cloning of Complementary DNA for the Alpha Subunit of the G Protein that Stimulates Adenylate Cyclase. Science, 229(4719), 1274â1277.

Authors 4

Bruce A. Harris (first)
Janet D. Robishaw (additional)
Susanne M. Mumby (additional)
Alfred G. Gilman (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 3:42 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 5:13 a.m.)
Indexed	3 months, 1 week ago (May 23, 2025, 6:51 a.m.)
Issued	39 years, 11 months ago (Sept. 20, 1985)
Published	39 years, 11 months ago (Sept. 20, 1985)
Published Print	39 years, 11 months ago (Sept. 20, 1985)

Funders 0

None

BibTeX

@article{Harris_1985, title={Molecular Cloning of Complementary DNA for the Alpha Subunit of the G Protein that Stimulates Adenylate Cyclase}, volume={229}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3839937}, DOI={10.1126/science.3839937}, number={4719}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Harris, Bruce A. and Robishaw, Janet D. and Mumby, Susanne M. and Gilman, Alfred G.}, year={1985}, month=sep, pages={1274–1277} }

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