DOI: 10.1126/science.3685996. Identification of the Iron-Responsive Element for the Translational Regulation of Human Ferritin mRNA

Identification of the Iron-Responsive Element for the Translational Regulation of Human Ferritin mRNA

10.1126/science.3685996

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Regulated translation of messenger RNA offers an important mechanism for the control of gene expression. The biosynthesis of the intracellular iron storage protein ferritin is translationally regulated by iron. A cis -acting element that is both necessary and sufficient for this translational regulation is present within the 5′ nontranslated leader region of the human ferritin H-chain messenger RNA. In this report the iron-responsive element (IRE) was identified by deletional analysis. Moreover, a synthetic oligodeoxynucleotide was shown to be able to transfer iron regulation to a construct that would otherwise not be able to respond to iron. The IRE has been highly conserved and predates the evolutionary segregation between amphibians, birds, and man. The IRE may prove to be useful for the design of translationally regulated expression systems.

Bibliography

Hentze, M. W., Caughman, S. W., Rouault, T. A., Barriocanal, J. G., Dancis, A., Harford, J. B., & Klausner, R. D. (1987). Identification of the Iron-Responsive Element for the Translational Regulation of Human Ferritin mRNA. Science, 238(4833), 1570â1573.

Authors 7

Matthias W. Hentze (first)
S. Wright Caughman (additional)
Tracey A. Rouault (additional)
Javier G. Barriocanal (additional)
Andrew Dancis (additional)
Joe B. Harford (additional)
Richard D. Klausner (additional)

References 30 Referenced 431

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HENTZE, M.W., A CIS-ACTING ELEMENT IS NECESSARY AND SUFFICIENT FOR TRANSLATIONAL REGULATION OF HUMAN FERRITIN EXPRESSION IN RESPONSE TO IRON, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 84: 6730 (1987). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1987)
HENTZE, M.W., CLONING, CHARACTERIZATION, EXPRESSION, AND CHROMOSOMAL LOCALIZATION OF A HUMAN FERRITIN HEAVY-CHAIN GENE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 7226 (1986). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1986)
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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 4:28 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 5:23 a.m.)
Indexed	41 minutes ago (Aug. 25, 2025, 11:02 p.m.)
Issued	37 years, 8 months ago (Dec. 11, 1987)
Published	37 years, 8 months ago (Dec. 11, 1987)
Published Print	37 years, 8 months ago (Dec. 11, 1987)

Funders 0

None

BibTeX

@article{Hentze_1987, title={Identification of the Iron-Responsive Element for the Translational Regulation of Human Ferritin mRNA}, volume={238}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3685996}, DOI={10.1126/science.3685996}, number={4833}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Hentze, Matthias W. and Caughman, S. Wright and Rouault, Tracey A. and Barriocanal, Javier G. and Dancis, Andrew and Harford, Joe B. and Klausner, Richard D.}, year={1987}, month=dec, pages={1570–1573} }

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