DOI: 10.1126/science.3685975. Purification and Properties of <i>Drosophila</i> Heat Shock Activator Protein

Purification and Properties of Drosophila Heat Shock Activator Protein

10.1126/science.3685975

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Drosophila heat shock activator protein, a rare transacting factor which is induced upon heat shock to bind specifically to the heat shock regulatory sequence in vivo, has been purified from shocked cells to more than 95 percent homogeneity by sequence-specific duplex oligonucleotide affinity chromatography. The purified protein has a relative molecular mass of 110 kilodaltons, binds to the regulatory sequence with great affinity and specificity, and strongly stimulates transcription of the Drosophila hsp70 gene. Studies with this regulatory protein should lead to an understanding of the biochemical pathway underlying the heat shock phenomenon.

Bibliography

Wu, C., Wilson, S., Walker, B., Dawid, I., Paisley, T., Zimarino, V., & Ueda, H. (1987). Purification and Properties of Drosophila Heat Shock Activator Protein. Science, 238(4831), 1247â1253.

Authors 7

Carl Wu (first)
Susan Wilson (additional)
Barbara Walker (additional)
Igor Dawid (additional)
Thomas Paisley (additional)
Vincenzo Zimarino (additional)
Hitoshi Ueda (additional)

References 44 Referenced 272

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 4:28 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 5:21 a.m.)
Indexed	11 months, 2 weeks ago (Sept. 11, 2024, 1:53 a.m.)
Issued	37 years, 9 months ago (Nov. 27, 1987)
Published	37 years, 9 months ago (Nov. 27, 1987)
Published Print	37 years, 9 months ago (Nov. 27, 1987)

Funders 0

None

BibTeX

@article{Wu_1987, title={Purification and Properties of Drosophila Heat Shock Activator Protein}, volume={238}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3685975}, DOI={10.1126/science.3685975}, number={4831}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Wu, Carl and Wilson, Susan and Walker, Barbara and Dawid, Igor and Paisley, Thomas and Zimarino, Vincenzo and Ueda, Hitoshi}, year={1987}, month=nov, pages={1247–1253} }

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