DOI: 10.1126/science.36664. Cellular Applications of <sup>31</sup> P and <sup>13</sup> C Nuclear Magnetic Resonance

Cellular Applications of 31 P and 13 C Nuclear Magnetic Resonance

10.1126/science.36664

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

High-resolution nuclear magnetic resonance (NMR) studies of cells and purified mitochondria are discussed to show the kind of information that can be obtained in vivo. In suspensions of Escherichia coli both phosphorus-31 and carbon-13 NMR studies of glycolysis and bioenergetics are presented. In rat liver cells the pathways of gluconeogenesis from carbon-13-labeled glycerol are followed by carbon-13 NMR. In the intact liver cells cytosolic and mitochondrial p H's were separately measured by phosphorus-31 NMR. In purified mitochondria the internal and external concentrations of inorganic phosphate, adenosine diphosphate, and adenosine triphosphate were determined by phosphorus-31 NMR while the p H difference across the membrane was measured simultaneously.

Bibliography

Shulman, R. G., Brown, T. R., Ugurbil, K., Ogawa, S., Cohen, S. M., & den Hollander, J. A. (1979). Cellular Applications of 31 P and 13 C Nuclear Magnetic Resonance. Science, 205(4402), 160â166.

Authors 6

R. G. Shulman (first)
T. R. Brown (additional)
K. Ugurbil (additional)
S. Ogawa (additional)
S. M. Cohen (additional)
J. A. den Hollander (additional)

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Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 12:39 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 4:47 a.m.)
Indexed	1 day, 11 hours ago (Sept. 4, 2025, 10:20 a.m.)
Issued	46 years, 1 month ago (July 13, 1979)
Published	46 years, 1 month ago (July 13, 1979)
Published Print	46 years, 1 month ago (July 13, 1979)

Funders 0

None

BibTeX

@article{Shulman_1979, title={Cellular Applications of 31 P and 13 C Nuclear Magnetic Resonance}, volume={205}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.36664}, DOI={10.1126/science.36664}, number={4402}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Shulman, R. G. and Brown, T. R. and Ugurbil, K. and Ogawa, S. and Cohen, S. M. and den Hollander, J. A.}, year={1979}, month=jul, pages={160–166} }

JSON

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