Affinity Chromatography of Splicing Complexes: U2, U5, and U4 + U6 Small Nuclear Ribonucleoprotein Particles in the Spliceosome
10.1126/science.3638792
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

The splicing process, which removes intervening sequences from messenger RNA (mRNA) precursors is essential to gene expression in eukaryotic cells. This site-specific process requires precise sequence recognition at the boundaries of an intervening sequence, but the mechanism of this recognition is not understood. The splicing of mRNA precursors occurs in a multicomponent complex termed the spliceosome. Such an assembly of components is likely to play a key role in specifying those sequences to be spliced. In order to analyze spliceosome structure, a stringent approach was developed to obtain splicing complexes free of cellular contaminants. This approach is a form of affinity chromatography based on the high specificity of the biotin-streptavidin interaction. A minimum of three subunits: U2, U5, and U4 + U6 small nuclear ribonucleoprotein particles were identified in the 35 S spliceosome structure, which also contains the bipartite RNA intermediate of splicing. A 25 S presplicing complex contained only the U2 particle. The multiple subunit structure of the spliceosome has implications for the regulation of a splicing event and for its possible catalysis by ribozyme or ribozymes.

Bibliography

Grabowski, P. J., & Sharp, P. A. (1986). Affinity Chromatography of Splicing Complexes: U2, U5, and U4 + U6 Small Nuclear Ribonucleoprotein Particles in the Spliceosome. Science, 233(4770), 1294–1299.

Authors 2
  1. Paula J. Grabowski (first)
  2. Phillip A. Sharp (additional)
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Dates
Type When
Created 18 years, 10 months ago (Oct. 5, 2006, 4:28 p.m.)
Deposited 1 year, 7 months ago (Jan. 14, 2024, 4:46 a.m.)
Indexed 11 months, 4 weeks ago (Sept. 3, 2024, 11:46 a.m.)
Issued 38 years, 11 months ago (Sept. 19, 1986)
Published 38 years, 11 months ago (Sept. 19, 1986)
Published Print 38 years, 11 months ago (Sept. 19, 1986)
Funders 0

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@article{Grabowski_1986, title={Affinity Chromatography of Splicing Complexes: U2, U5, and U4 + U6 Small Nuclear Ribonucleoprotein Particles in the Spliceosome}, volume={233}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3638792}, DOI={10.1126/science.3638792}, number={4770}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Grabowski, Paula J. and Sharp, Phillip A.}, year={1986}, month=sep, pages={1294–1299} }