DOI: 10.1126/science.3547651. Molecular Cloning and Expression of a Human B-Cell Growth Factor Gene in <i>Escherichia coli</i>

Molecular Cloning and Expression of a Human B-Cell Growth Factor Gene in Escherichia coli

10.1126/science.3547651

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A human B-cell growth factor (BCGF) (12 kilodaltons) supports the clonal proliferation of B lymphocytes. A clone was isolated that contained the proper structural sequence to encode biologically active, 12-kilodalton BCGF in Escherichia coli and to hybridize to a specific messenger RNA, identified by in vitro translation in Xenopus laevis oocytes. A relatively hydrophobic region of 18 amino acids was found at the amino terminal of the 124-amino acid-long polypeptide. The carboxyl terminal is composed of at least 32 amino acids that are derived from nucleotide sequences bearing significant homology to the Alu repeat family.

Bibliography

Sharma, S., Mehta, S., Morgan, J., & Maizel, A. (1987). Molecular Cloning and Expression of a Human B-Cell Growth Factor Gene in Escherichia coli. Science, 235(4795), 1489â1492.

Authors 4

Surendra Sharma (first)
Shashi Mehta (additional)
John Morgan (additional)
Abby Maizel (additional)

References 33 Referenced 64

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HEIDECKER, G, SEQUENCE-ANALYSIS OF ZEIN CDNAS OBTAINED BY AN EFFICIENT MESSENGER-RNA CLONING METHOD, NUCLEIC ACIDS RESEARCH 11: 4891 (1983). (10.1093/nar/11.14.4891) / NUCLEIC ACIDS RESEARCH (1983)
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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 4:28 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 4:57 a.m.)
Indexed	1 year, 7 months ago (Jan. 15, 2024, 12:26 a.m.)
Issued	38 years, 5 months ago (March 20, 1987)
Published	38 years, 5 months ago (March 20, 1987)
Published Print	38 years, 5 months ago (March 20, 1987)

Funders 0

None

BibTeX

@article{Sharma_1987, title={Molecular Cloning and Expression of a Human B-Cell Growth Factor Gene in Escherichia coli}, volume={235}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3547651}, DOI={10.1126/science.3547651}, number={4795}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Sharma, Surendra and Mehta, Shashi and Morgan, John and Maizel, Abby}, year={1987}, month=mar, pages={1489–1492} }

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