DOI: 10.1126/science.3538420. Separation of Large DNA Molecules by Contour-Clamped Homogeneous Electric Fields

Separation of Large DNA Molecules by Contour-Clamped Homogeneous Electric Fields

10.1126/science.3538420

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Electric fields can be manipulated by a method in which multiple electrodes are arranged along a closed contour and clamped to predetermined electric potentials. This method may be applied to a broad range of problems in the separation of macromolecules by gel electrophoresis. DNA molecules as large as 2 megabases can be well separated with a contour-clamped homogeneous electric field alternating between two orientations 120° apart. The pattern of separation is independent of position in the gel, which is an advantage over previous methods. DNA less than 50 kilobases can be separated without distortion even at high voltage with a nonalternating contour-clamped homogeneous field. Decreased band broadening in DNA less than 200 bases can be achieved with a contour-clamped inhomogeneous field.

Bibliography

Chu, G., Vollrath, D., & Davis, R. W. (1986). Separation of Large DNA Molecules by Contour-Clamped Homogeneous Electric Fields. Science, 234(4783), 1582â1585.

Authors 3

Gilbert Chu (first)
Douglas Vollrath (additional)
Ronald W. Davis (additional)

References 14 Referenced 1,057

BELL, L, SEPARATION OF BRANCHED FROM LINEAR DNA BY TWO-DIMENSIONAL GEL-ELECTROPHORESIS, ANALYTICAL BIOCHEMISTRY 130: 527 (1983). (10.1016/0003-2697(83)90628-0) / ANALYTICAL BIOCHEMISTRY (1983)
BONCINELLI, E, AN AGAROSE-GEL RESOLVING A WIDE-RANGE OF DNA FRAGMENT LENGTHS, ANALYTICAL BIOCHEMISTRY 134: 40 (1983). (10.1016/0003-2697(83)90260-9) / ANALYTICAL BIOCHEMISTRY (1983)
10.1093/nar/12.14.5647
CARLE, G.F., AN ELECTROPHORETIC KARYOTYPE FOR YEAST, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 82: 3756 (1985). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1985)
10.1126/science.3952500
EIBEL, H, PREFERENTIAL INTEGRATION OF YEAST TRANSPOSABLE ELEMENT TY INTO A PROMOTER REGION, NATURE 307: 386 (1984). (10.1038/307386a0) / NATURE (1984)
FANGMAN, W.L., SEPARATION OF VERY LARGE DNA-MOLECULES BY GEL-ELECTROPHORESIS, NUCLEIC ACIDS RESEARCH 5: 653 (1978). (10.1093/nar/5.3.653) / NUCLEIC ACIDS RESEARCH (1978)
10.1016/0092-8674(85)90152-7
JOHNSON, P.H., ELECTROPHORESIS OF DNA IN AGAROSE GELS - OPTIMIZING SEPARATIONS OF CONFORMATIONAL ISOMERS OF DOUBLE-STRANDED AND SINGLE-STRANDED DNAS, BIOCHEMISTRY 16: 4217 (1977). (10.1021/bi00638a014) / BIOCHEMISTRY (1977)
LUMPKIN, O.J., THEORY OF GEL-ELECTROPHORESIS OF DNA, BIOPOLYMERS 24: 1573 (1985). (10.1002/bip.360240812) / BIOPOLYMERS (1985)
Maniatis, T., Molecular Cloning: A Laboratory Manual: 153 (1982). / Molecular Cloning: A Laboratory Manual (1982)
MORTIMER, R.K., GENETIC-MAP OF SACCHAROMYCES-CEREVISIAE, EDITION-9, MICROBIOLOGICAL REVIEWS 49: 181 (1985). (10.1128/mr.49.3.181-213.1985) / MICROBIOLOGICAL REVIEWS (1985)
Purcell, E. M., Electricity and Magnetism: 40 (1965). / Electricity and Magnetism (1965)
10.1016/0092-8674(84)90301-5

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 4:28 p.m.)
Deposited	1 year, 7 months ago (Jan. 14, 2024, 5:17 a.m.)
Indexed	3 weeks, 4 days ago (July 30, 2025, 8:25 p.m.)
Issued	38 years, 8 months ago (Dec. 19, 1986)
Published	38 years, 8 months ago (Dec. 19, 1986)
Published Print	38 years, 8 months ago (Dec. 19, 1986)

Funders 0

None

BibTeX

@article{Chu_1986, title={Separation of Large DNA Molecules by Contour-Clamped Homogeneous Electric Fields}, volume={234}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3538420}, DOI={10.1126/science.3538420}, number={4783}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Chu, Gilbert and Vollrath, Douglas and Davis, Ronald W.}, year={1986}, month=dec, pages={1582–1585} }

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