DOI: 10.1126/science.3420415. Dynamic Instability of Sheared Microtubules Observed by Quasi-Elastic Light Scattering

Dynamic Instability of Sheared Microtubules Observed by Quasi-Elastic Light Scattering

10.1126/science.3420415

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The kinetics of microtubule reassembly was studied in vitro by quasi-elastic light scattering (QELS). When microtubules assembled in the absence of microtubule-associated proteins (MAPs) were sheared, they rapidly depolymerized, recovered, and reassembled. The mean length of the recovered microtubules was the same as that observed just before shearing, implying that on average one fragment per original microtubule survived the fragmentation and recovery. When microtubules that contained 25 percent brain MAP were sheared, the fragments did not depolymerize extensively and the average length of the fragments decreased by a factor of 3 relative to the unsheared sample. The results support the dynamic instability model, which predicts that cellular microtubules are latently unstable structures protected on their ends by stabilizing caps.

Bibliography

Keates, R. A. B., & Hallett, F. R. (1988). Dynamic Instability of Sheared Microtubules Observed by Quasi-Elastic Light Scattering. Science, 241(4873), 1642â1645.

Authors 2

Robert A. B. Keates (first)
F. Ross Hallett (additional)

References 16 Referenced 13

10.1016/S0021-9258(18)66704-3
Carlier, M. F., Proceedings of the National Academy of Sciences of the United States of America 81: 771 (1984). / Proceedings of the National Academy of Sciences of the United States of America (1984)
10.1073/pnas.82.4.1131
10.1002/bip.360240604
10.1002/bip.360241216
10.1038/321605a0
10.1139/o84-102
10.1016/0092-8674(86)90318-1
10.1083/jcb.102.3.1007
10.1038/312232a0
10.1038/312237a0
10.1021/la00064a016
10.1083/jcb.105.2.863
10.1083/jcb.104.3.395
10.1083/jcb.104.2.277
WALKER, R.A., JOURNAL OF CELL BIOLOGY 105: 29a (1987). (10.1083/jcb.105.1.29) / JOURNAL OF CELL BIOLOGY (1987)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 10:04 p.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 4:38 p.m.)
Issued	36 years, 11 months ago (Sept. 23, 1988)
Published	36 years, 11 months ago (Sept. 23, 1988)
Published Print	36 years, 11 months ago (Sept. 23, 1988)

Funders 0

None

BibTeX

@article{Keates_1988, title={Dynamic Instability of Sheared Microtubules Observed by Quasi-Elastic Light Scattering}, volume={241}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3420415}, DOI={10.1126/science.3420415}, number={4873}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Keates, Robert A. B. and Hallett, F. Ross}, year={1988}, month=sep, pages={1642–1645} }

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