DOI: 10.1126/science.3413476. A Ligase-Mediated Gene Detection Technique

A Ligase-Mediated Gene Detection Technique

10.1126/science.3413476

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

An assay for the presence of given DNA sequences has been developed, based on the ability of two oligonucleotides to anneal immediately adjacent to each other on a complementary target DNA molecule. The two oligonucleotides are then joined covalently by the action of a DNA ligase, provided that the nucleotides at the junction are correctly base-paired. Thus single nucleotide substitutions can be distinguished. This strategy permits the rapid and standardized identification of single-copy gene sequences in genomic DNA.

Bibliography

Landegren, U., Kaiser, R., Sanders, J., & Hood, L. (1988). A Ligase-Mediated Gene Detection Technique. Science, 241(4869), 1077â1080.

Authors 4

Ulf Landegren (first)
Robert Kaiser (additional)
Jane Sanders (additional)
Leroy Hood (additional)

References 25 Referenced 598

Whiteley N. M. European Patent application 0 185 494 A2 (1986).
BOWTELL, DDL, RAPID ISOLATION OF EUKARYOTIC DNA, ANALYTICAL BIOCHEMISTRY 162: 463 (1987). (10.1016/0003-2697(87)90421-0) / ANALYTICAL BIOCHEMISTRY (1987)
10.1126/science.3296189
CONNELL, C, AUTOMATED DNA-SEQUENCE ANALYSIS, BIOTECHNIQUES 5: 342 (1987). / BIOTECHNIQUES (1987)
Conner, B. J., Proceedings of the National Academy of Sciences of the United States of America 80: 278 (1983). / Proceedings of the National Academy of Sciences of the United States of America (1983)
COOPER, D.N., HUMAN-GENE CLONING AND DISEASE ANALYSIS, LANCET 1: 273 (1987). / LANCET (1987)
Geever, R. F., Proceedings of the National Academy of Sciences of the United States of America 78: 5081 (1981). / Proceedings of the National Academy of Sciences of the United States of America (1981)
HORVATH, S.J., AN AUTOMATED DNA SYNTHESIZER EMPLOYING DEOXYNUCLEOSIDE 3'-PHOSPHORAMIDITES, METHODS IN ENZYMOLOGY 154: 314 (1987). (10.1016/0076-6879(87)54082-4) / METHODS IN ENZYMOLOGY (1987)
HUNKAPILLER T COMMUNICATION.
KARK, J.A., SICKLE-CELL TRAIT AS A RISK FACTOR FOR SUDDEN-DEATH IN PHYSICAL-TRAINING, NEW ENGLAND JOURNAL OF MEDICINE 317: 781 (1987). (10.1056/NEJM198709243171301) / NEW ENGLAND JOURNAL OF MEDICINE (1987)
McKusick V. A. Mendelian Inheritance in Man 7 (1986).
MYERS, R.M., DETECTION OF SINGLE BASE SUBSTITUTIONS IN TOTAL GENOMIC DNA, NATURE 313: 495 (1985). (10.1038/313495a0) / NATURE (1985)
10.1126/science.4071043
ROTTER, J.I., WHAT MAINTAINS THE FREQUENCIES OF HUMAN GENETIC-DISEASES, NATURE 329: 289 (1987). (10.1038/329289a0) / NATURE (1987)
SAIKI, R.K., A NOVEL METHOD FOR THE DETECTION OF POLYMORPHIC RESTRICTION SITES BY CLEAVAGE OF OLIGONUCLEOTIDE PROBES - APPLICATION TO SICKLE-CELL-ANEMIA, BIO-TECHNOLOGY 3: 1008 (1985). / BIO-TECHNOLOGY (1985)
SAIKI, R.K., ANALYSIS OF ENZYMATICALLY AMPLIFIED BETA-GLOBIN AND HLA-DQ-ALPHA DNA WITH ALLELE-SPECIFIC OLIGONUCLEOTIDE PROBES, NATURE 324: 163 (1986). (10.1038/324163a0) / NATURE (1986)
10.1126/science.2999980
SMITH, L.M., FLUORESCENCE DETECTION IN AUTOMATED DNA-SEQUENCE ANALYSIS, NATURE 321: 674 (1986). (10.1038/321674a0) / NATURE (1986)
SMITH, L.M., THE SYNTHESIS OF OLIGONUCLEOTIDES CONTAINING AN ALIPHATIC AMINO GROUP AT THE 5' TERMINUS - SYNTHESIS OF FLUORESCENT DNA PRIMERS FOR USE IN DNA-SEQUENCE ANALYSIS, NUCLEIC ACIDS RESEARCH 13: 2399 (1985). (10.1093/nar/13.7.2399) / NUCLEIC ACIDS RESEARCH (1985)
SOINI, E, TIME-RESOLVED FLUOROMETER FOR LANTHANIDE CHELATES - A NEW GENERATION OF NON-ISOTOPIC IMMUNOASSAYS, CLINICAL CHEMISTRY 29: 65 (1983). (10.1093/clinchem/29.1.65) / CLINICAL CHEMISTRY (1983)
SVEJGAARD, A, HLA AND DISEASE 1982 - A SURVEY, IMMUNOLOGICAL REVIEWS 70: 193 (1983). (10.1111/j.1600-065X.1983.tb00715.x) / IMMUNOLOGICAL REVIEWS (1983)
Tiwari J. L. HLA and Disease Associations (1985). (10.1007/978-1-4613-8545-5)
WETMUR, J.G., KINETICS OF RENATURATION OF DNA, JOURNAL OF MOLECULAR BIOLOGY 31: 349 (1968). (10.1016/0022-2836(68)90414-2) / JOURNAL OF MOLECULAR BIOLOGY (1968)
Winslow, R. M., The Metabolic Basis of Inheritance: 1666 (1983). / The Metabolic Basis of Inheritance (1983)
10.1073/pnas.82.22.7575

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 10:07 p.m.)
Indexed	1 week, 6 days ago (Aug. 12, 2025, 6:24 p.m.)
Issued	37 years ago (Aug. 26, 1988)
Published	37 years ago (Aug. 26, 1988)
Published Print	37 years ago (Aug. 26, 1988)

Funders 0

None

BibTeX

@article{Landegren_1988, title={A Ligase-Mediated Gene Detection Technique}, volume={241}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3413476}, DOI={10.1126/science.3413476}, number={4869}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Landegren, Ulf and Kaiser, Robert and Sanders, Jane and Hood, Leroy}, year={1988}, month=aug, pages={1077–1080} }

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