DOI: 10.1126/science.3399893. Selective Activation of Transcription by a Novel CCAAT Binding Factor

Selective Activation of Transcription by a Novel CCAAT Binding Factor

10.1126/science.3399893

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A novel CCAAT binding factor (CBF) composed of two different subunits has been extensively purified from rat liver. Both subunits are needed for specific binding to DNA. Addition of this purified protein to nuclear extracts of NIH 3T3 fibroblasts stimulates transcription from several promoters including the α2(I) collagen, the α1(I) collagen, the Rous sarcoma virus long terminal repeat (RSV-LTR), and the adenovirus major late promoter. Point mutations in the CCAAT motif that show either no binding or a decreased binding of CBF likewise abolish or reduce activation of transcription by CBF. Activation of transcription requires, therefore, the specific binding of CBF to its recognition sites.

Bibliography

Maity, S. N., Golumbek, P. T., Karsenty, G., & de Crombrugghe, B. (1988). Selective Activation of Transcription by a Novel CCAAT Binding Factor. Science, 241(4865), 582â585.

Authors 4

Sankar N. Maity (first)
Paul T. Golumbek (additional)
Gerard Karsenty (additional)
Benoit de Crombrugghe (additional)

References 31 Referenced 155

BAGCHI, M.K., PURIFICATION AND CHARACTERIZATION OF CHICKEN OVALBUMIN GENE UPSTREAM PROMOTER TRANSCRIPTION FACTOR FROM HOMOLOGOUS OVIDUCT CELLS, MOLECULAR AND CELLULAR BIOLOGY 7: 4151 (1987). / MOLECULAR AND CELLULAR BIOLOGY (1987)
10.1016/0092-8674(88)90483-7
CHODOSH, L.A., A YEAST AND A HUMAN CCAAT-BINDING PROTEIN HAVE HETEROLOGOUS SUBUNITS THAT ARE FUNCTIONALLY INTERCHANGEABLE, CELL 53: 25 (1988). (10.1016/0092-8674(88)90484-9) / CELL (1988)
COHEN, R.B., PARTIAL-PURIFICATION OF A NUCLEAR-PROTEIN THAT BINDS TO THE CCAAT BOX OF THE MOUSE ALPHA-GLOBIN GENE, MOLECULAR AND CELLULAR BIOLOGY 6: 821 (1986). / MOLECULAR AND CELLULAR BIOLOGY (1986)
10.1093/nar/11.5.1475
DORN, A, A MULTIPLICITY OF CCAAT BOX-BINDING PROTEINS, CELL 50: 863 (1987). (10.1016/0092-8674(87)90513-7) / CELL (1987)
DYNAN, W.S., CONTROL OF EUKARYOTIC MESSENGER-RNA SYNTHESIS BY SEQUENCE-SPECIFIC DNA-BINDING PROTEINS, NATURE 316: 774 (1985). (10.1038/316774a0) / NATURE (1985)
10.1093/nar/9.23.6505
GALAS, D.J., DNAASE FOOTPRINTING - SIMPLE METHOD FOR DETECTION OF PROTEIN-DNA BINDING SPECIFICITY, NUCLEIC ACIDS RESEARCH 5: 3157 (1978). (10.1093/nar/5.9.3157) / NUCLEIC ACIDS RESEARCH (1978)
10.1093/nar/9.13.3047
GORMAN, C, EUKARYOTIC GENE EXPR: 129 (1982). / EUKARYOTIC GENE EXPR (1982)
GRAVES, B.J., HOMOLOGOUS RECOGNITION OF A PROMOTER DOMAIN COMMON TO THE MSV LTR AND THE HSV TK GENE, CELL 44: 565 (1986). (10.1016/0092-8674(86)90266-7) / CELL (1986)
Harbers, K., Proceedings of the National Academy of Sciences of the United States of America 81: 1504 (1984). / Proceedings of the National Academy of Sciences of the United States of America (1984)
HATAMOCHI, A, DIFFERENTIAL BINDING OF A CCAAT DNA-BINDING FACTOR TO THE PROMOTERS OF THE MOUSE ALPHA-2(I) AND ALPHA-1(III) COLLAGEN GENES, JOURNAL OF BIOLOGICAL CHEMISTRY 261: 11310 (1986). (10.1016/S0021-9258(18)67384-3) / JOURNAL OF BIOLOGICAL CHEMISTRY (1986)
HATAMOCHI, A, A CCAAT DNA-BINDING FACTOR CONSISTING OF 2 DIFFERENT COMPONENTS THAT ARE BOTH REQUIRED FOR DNA-BINDING, JOURNAL OF BIOLOGICAL CHEMISTRY 263: 5940 (1988). (10.1016/S0021-9258(18)60657-X) / JOURNAL OF BIOLOGICAL CHEMISTRY (1988)
HOPE, I.A., FUNCTIONAL DISSECTION OF A EUKARYOTIC TRANSCRIPTIONAL ACTIVATOR PROTEIN, GCN4 OF YEAST, CELL 46: 885 (1986). (10.1016/0092-8674(86)90070-X) / CELL (1986)
Hu, S. L., Proceedings of the National Academy of Sciences of the United States of America 78: 820 (1981). / Proceedings of the National Academy of Sciences of the United States of America (1981)
JONES, K.A., A CELLULAR DNA-BINDING PROTEIN THAT ACTIVATES EUKARYOTIC TRANSCRIPTION AND DNA-REPLICATION, CELL 48: 79 (1987). (10.1016/0092-8674(87)90358-8) / CELL (1987)
Karsenty, G., Journal of Biological Chemistry 263: 13909 (1988). (10.1016/S0021-9258(18)68330-9) / Journal of Biological Chemistry (1988)
10.1126/science.3080805
KUNKEL, T.A., RAPID AND EFFICIENT SITE-SPECIFIC MUTAGENESIS WITHOUT PHENOTYPIC SELECTION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 82: 488 (1985). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1985)
MA, J, DELETION ANALYSIS OF GAL4 DEFINES 2 TRANSCRIPTIONAL ACTIVATING SEGMENTS, CELL 48: 847 (1987). (10.1016/0092-8674(87)90081-X) / CELL (1987)
10.1126/science.3296191
MUDRYJ M THESIS G WASHINGTON (1988).
10.1126/science.3457470
OIKARINEN, J, SEPARATE BINDING-SITES FOR NUCLEAR FACTOR-I AND A CCAAT DNA-BINDING FACTOR IN THE MOUSE ALPHA-2(I) COLLAGEN PROMOTER, JOURNAL OF BIOLOGICAL CHEMISTRY 262: 11064 (1987). (10.1016/S0021-9258(18)60927-5) / JOURNAL OF BIOLOGICAL CHEMISTRY (1987)
PTASHNE M GENETIC SWITCH (1986).
ROSSI, P, IDENTIFICATION OF A CELL-SPECIFIC TRANSCRIPTIONAL ENHANCER IN THE 1ST INTRON OF THE MOUSE-ALPHA-2 (TYPE-I) COLLAGEN GENE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 84: 5590 (1987). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1987)
SAWADOGO, M, INTERACTION OF A GENE-SPECIFIC TRANSCRIPTION FACTOR WITH THE ADENOVIRUS MAJOR LATE PROMOTER UPSTREAM OF THE TATA BOX REGION, CELL 43: 165 (1985). (10.1016/0092-8674(85)90021-2) / CELL (1985)
TSAI, S.Y., INTERACTIONS BETWEEN A DNA-BINDING TRANSCRIPTION FACTOR (COUP) AND A NON-DNA BINDING-FACTOR (S300-II), CELL 50: 701 (1987). (10.1016/0092-8674(87)90328-X) / CELL (1987)
WEEKENS, M.P., J BIOL CHEM 253: 2483 (1978). (10.1016/S0021-9258(17)38098-5) / J BIOL CHEM (1978)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 10:09 p.m.)
Indexed	1 year, 1 month ago (July 16, 2024, 9:33 p.m.)
Issued	37 years ago (July 29, 1988)
Published	37 years ago (July 29, 1988)
Published Print	37 years ago (July 29, 1988)

Funders 0

None

BibTeX

@article{Maity_1988, title={Selective Activation of Transcription by a Novel CCAAT Binding Factor}, volume={241}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3399893}, DOI={10.1126/science.3399893}, number={4865}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Maity, Sankar N. and Golumbek, Paul T. and Karsenty, Gerard and de Crombrugghe, Benoit}, year={1988}, month=jul, pages={582–585} }

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