DOI: 10.1126/science.322276. Recombinational Switch for Gene Expression

Recombinational Switch for Gene Expression

10.1126/science.322276

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Flagellar antigens are specified by two genes, H1 and H2. The expression of these genes is regulated such that only one gene activity, or phase, is expressed at a given time. Molecular cloning techniques were used to isolate the segments of Salmonella DNA which contain these genetic loci. Heteroduplex analyses revealed an anomaly in the cloned fragment, that is, an apparent inversion, which was shown to be adjacent to the H2 gene. A correlation was demonstrated between the phase state of the H2 gene and the sequence of the adjacent segment. We propose that an inversion of this region is the phase-determining event in flagellar gene expression in Salmonella .

Bibliography

Zieg, J., Silverman, M., Hilmen, M., & Simon, M. (1977). Recombinational Switch for Gene Expression. Science, 196(4286), 170â172.

Authors 4

Janine Zieg (first)
Michael Silverman (additional)
Marcia Hilmen (additional)
Melvin Simon (additional)

References 19 Referenced 238

Andrewes, F.W., Journal of Pathology and Bacteriology 25: 505 (1922). (10.1002/path.1700250411) / Journal of Pathology and Bacteriology (1922)
10.1126/science.847462
FUJITA, H, STUDIES ON H-O VARIANTS IN SALMONELLA IN RELATION TO PHASE VARIATION, JOURNAL OF GENERAL MICROBIOLOGY 76: 127 (1973). (10.1099/00221287-76-1-127) / JOURNAL OF GENERAL MICROBIOLOGY (1973)
HERSHFIELD, V, PLASMID COLE1 AS A MOLECULAR VEHICLE FOR CLONING AND AMPLIFICATION OF DNA, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 71: 3455 (1974). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1974)
Hicks, J. B., Genetics 85: 373 (1977). / Genetics (1977)
10.1126/science.187.4173.226
IINO, T, GENETICS AND CHEMISTRY OF BACTERIAL FLAGELLA, BACTERIOLOGICAL REVIEWS 33: 454 (1969). (10.1128/br.33.4.454-475.1969) / BACTERIOLOGICAL REVIEWS (1969)
KONDOH, H, BIOCHIMICA ET BIOPHYSICA ACTA 336: 117 (1974). (10.1016/0005-2795(74)90390-0) / BIOCHIMICA ET BIOPHYSICA ACTA (1974)
Lederberg, J., Genetics 41: 743 (1956). (10.1093/genetics/41.5.743) / Genetics (1956)
MCCLINTOCK, B, CONTROLLING ELEMENTS AND THE GENE, COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY 21: 197 (1956). (10.1101/SQB.1956.021.01.017) / COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY (1956)
MCCLINTOCK B SYMPOSIUM OF THE SOCIETY FOR DEVELOPMENTAL BIOLOGY 184 (1967).
PEARCE, U.B., PHASE VARIATION OF FLAGELLAR ANTIGENS IN SALMONELLA - ABORTIVE TRANSDUCTION STUDIES, JOURNAL OF GENERAL MICROBIOLOGY 49: 335 (1967). (10.1099/00221287-49-2-335) / JOURNAL OF GENERAL MICROBIOLOGY (1967)
SAEDLER, H, MOLECULAR & GENERAL GENETICS 132: 265 (1974). (10.1007/BF00268569) / MOLECULAR & GENERAL GENETICS (1974)
SILVERMAN, M, IDENTIFICATION OF MOT GENE PRODUCT WITH ESCHERICHIA-COLI LAMBDA HYBRIDS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 73: 3126 (1976). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1976)
10.1093/genetics/81.4.595
STOCKER, BAD, MEASUREMENTS OF RATE OF MUTATION OF FLAGELLAR ANTIGENIC PHASE IN SALMONELLA-TYPHI-MURIUM, JOURNAL OF HYGIENE 47: 398 (1949). / JOURNAL OF HYGIENE (1949)
Tonegawa, S., Cold Spring Harbor Symposia on Quantitative Biology 41: 877 (1977). (10.1101/SQB.1977.041.01.097) / Cold Spring Harbor Symposia on Quantitative Biology (1977)
TOUSSAINT, A, DNA MODIFICATION FUNCTION OF TEMPERATE PHAGE MU-1, VIROLOGY 70: 17 (1976). (10.1016/0042-6822(76)90232-4) / VIROLOGY (1976)
Hicks J. B. DNA Insertion Elements Plasmids and Episomes (1977).

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 12:03 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 3:57 p.m.)
Indexed	1 month, 4 weeks ago (June 27, 2025, 5:23 a.m.)
Issued	48 years, 4 months ago (April 8, 1977)
Published	48 years, 4 months ago (April 8, 1977)
Published Print	48 years, 4 months ago (April 8, 1977)

Funders 0

None

BibTeX

@article{Zieg_1977, title={Recombinational Switch for Gene Expression}, volume={196}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.322276}, DOI={10.1126/science.322276}, number={4286}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Zieg, Janine and Silverman, Michael and Hilmen, Marcia and Simon, Melvin}, year={1977}, month=apr, pages={170–172} }

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