DOI: 10.1126/science.3194757. The Presence of Fibroblast Growth Factor in the Frog Egg: Its Role as a Natural Mesoderm Inducer

The Presence of Fibroblast Growth Factor in the Frog Egg: Its Role as a Natural Mesoderm Inducer

10.1126/science.3194757

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A complementary DNA clone corresponding to a 4.2-kilobase transcript that is present in the Xenopus oocyte and newly transcribed in the neurula stages of development has been isolated. This messenger RNA encodes a 155-amino acid protein that is 84% identical to the human basic fibroblast growth factor (bFGF). When expressed in Escherichia coli and purified, the Xenopus FGF induced mesoderm in animal cell blastomeres as measured by muscle actin expression. Immunoblots with an antibody to a Xenopus FGF peptide show that the oocyte and early embryo contain a store of the FGF polypeptide at high enough concentrations to induce mesoderm. The presence of FGF in the oocyte, together with the apparent lack of a secretory signal sequence in the protein, suggest that the regulation of mesoderm induction may involve novel mechanisms that occur after the translation of FGF.

Bibliography

Kimelman, D., Abraham, J. A., Haaparanta, T., Palisi, T. M., & Kirschner, M. W. (1988). The Presence of Fibroblast Growth Factor in the Frog Egg: Its Role as a Natural Mesoderm Inducer. Science, 242(4881), 1053â1056.

Authors 5

David Kimelman (first)
Judith A. Abraham (additional)
Tapio Haaparanta (additional)
Thomas M. Palisi (additional)
Marc W. Kirschner (additional)

References 29 Referenced 305

ABRAHAM, J.A., HUMAN BASIC FIBROBLAST GROWTH-FACTOR - NUCLEOTIDE-SEQUENCE AND GENOMIC ORGANIZATION, EMBO JOURNAL 5: 2523 (1986). (10.1002/j.1460-2075.1986.tb04530.x) / EMBO JOURNAL (1986)
10.1126/science.2425435
BAIRD, A, RECEPTOR-BINDING AND HEPARIN-BINDING DOMAINS OF BASIC FIBROBLAST GROWTH-FACTOR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 85: 2324 (1988). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1988)
Blam S. B. Oncogene in press.
COOKE, J, THE ORGANIZATION OF MESODERMAL PATTERN IN XENOPUS-LAEVIS - EXPERIMENTS USING A XENOPUS MESODERM-INDUCING FACTOR, DEVELOPMENT 101: 893 (1987). (10.1242/dev.101.4.893) / DEVELOPMENT (1987)
GOSPODAROWICZ, D, ISOLATION OF BRAIN FIBROBLAST GROWTH-FACTOR BY HEPARIN-SEPHAROSE AFFINITY-CHROMATOGRAPHY - IDENTITY WITH PITUITARY FIBROBLAST GROWTH-FACTOR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 81: 6963 (1984). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1984)
GURDON, J.B., ACTIVATION OF MUSCLE-SPECIFIC ACTIN GENES IN XENOPUS DEVELOPMENT BY AN INDUCTION BETWEEN ANIMAL AND VEGETAL CELLS OF A BLASTULA, CELL 41: 913 (1985). (10.1016/S0092-8674(85)80072-6) / CELL (1985)
JONES, E.A., THE DEVELOPMENT OF ANIMAL CAP CELLS IN XENOPUS - A MEASURE OF THE START OF ANIMAL CAP COMPETENCE TO FORM MESODERM, DEVELOPMENT 101: 557 (1987). (10.1242/dev.101.3.557) / DEVELOPMENT (1987)
KIMELMAN, D, SYNERGISTIC INDUCTION OF MESODERM BY FGF AND TGF-BETA AND THE IDENTIFICATION OF AN MESSENGER-RNA CODING FOR FGF IN THE EARLY XENOPUS EMBRYO, CELL 51: 869 (1987). (10.1016/0092-8674(87)90110-3) / CELL (1987)
KIMELMAN D unpublished data.
KINTNER, C.R., EXPRESSION OF XENOPUS N-CAM RNA IN ECTODERM IS AN EARLY RESPONSE TO NEURAL INDUCTION, DEVELOPMENT 99: 311 (1987). (10.1242/dev.99.3.311) / DEVELOPMENT (1987)
KLAGSBRUN, M, HUMAN-TUMOR CELLS SYNTHESIZE AN ENDOTHELIAL-CELL GROWTH-FACTOR THAT IS STRUCTURALLY RELATED TO BASIC FIBROBLAST GROWTH-FACTOR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 2448 (1986). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1986)
10.1093/nar/12.18.7057
LOBB, R.R., PURIFICATION OF 2 DISTINCT GROWTH-FACTORS FROM BOVINE NEURAL TISSUE BY HEPARIN AFFINITY-CHROMATOGRAPHY, BIOCHEMISTRY 23: 6295 (1984). (10.1021/bi00321a001) / BIOCHEMISTRY (1984)
10.1126/science.6382607
MITCHELL R COMMUNICATION.
MOSCATELLI, D, MR 25,000 HEPARIN-BINDING PROTEIN FROM GUINEA-PIG BRAIN IS A HIGH-MOLECULAR-WEIGHT FORM OF BASIC FIBROBLAST GROWTH-FACTOR, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 84: 5778 (1987). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1987)
Nieuwkoop P. D. The Epigenetic Nature of Early Chordate Development. Inductive Interaction and Competence (1985).
REBAGLIATI, M.R., IDENTIFICATION AND CLONING OF LOCALIZED MATERNAL RNAS FROM XENOPUS EGGS, CELL 42: 769 (1985). (10.1016/0092-8674(85)90273-9) / CELL (1985)
ROGELJ, S, BASIC FIBROBLAST GROWTH-FACTOR FUSED TO A SIGNAL PEPTIDE TRANSFORMS CELLS, NATURE 331: 173 (1988). (10.1038/331173a0) / NATURE (1988)
10.1126/science.3422517
ROSENBERG, A.H., VECTORS FOR SELECTIVE EXPRESSION OF CLONED DNAS BY T7 RNA-POLYMERASE, GENE 56: 125 (1987). (10.1016/0378-1119(87)90165-X) / GENE (1987)
SENO, M, STABILIZING BASIC FIBROBLAST GROWTH-FACTOR USING PROTEIN ENGINEERING, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 151: 701 (1988). (10.1016/S0006-291X(88)80337-1) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1988)
10.1126/science.6199844
SILVER S unpublished data.
SLACK, JMW, MESODERM INDUCTION IN EARLY XENOPUS EMBRYOS BY HEPARIN-BINDING GROWTH-FACTORS, NATURE 326: 197 (1987). (10.1038/326197a0) / NATURE (1987)
SMITH, J.C., A MESODERM-INDUCING FACTOR IS PRODUCED BY A XENOPUS CELL-LINE, DEVELOPMENT 99: 3 (1987). (10.1242/dev.99.1.3) / DEVELOPMENT (1987)
SOMMER, A, A FORM OF HUMAN BASIC FIBROBLAST GROWTH-FACTOR WITH AN EXTENDED AMINO TERMINUS, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 144: 543 (1987). (10.1016/S0006-291X(87)80001-3) / BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (1987)
WEEKS, D.L., A MATERNAL MESSENGER-RNA LOCALIZED TO THE VEGETAL HEMISPHERE IN XENOPUS EGGS CODES FOR A GROWTH-FACTOR RELATED TO TGF-BETA, CELL 51: 861 (1987). (10.1016/0092-8674(87)90109-7) / CELL (1987)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 4:23 p.m.)
Indexed	1 month ago (July 27, 2025, 3:48 a.m.)
Issued	36 years, 9 months ago (Nov. 18, 1988)
Published	36 years, 9 months ago (Nov. 18, 1988)
Published Print	36 years, 9 months ago (Nov. 18, 1988)

Funders 0

None

BibTeX

@article{Kimelman_1988, title={The Presence of Fibroblast Growth Factor in the Frog Egg: Its Role as a Natural Mesoderm Inducer}, volume={242}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3194757}, DOI={10.1126/science.3194757}, number={4881}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Kimelman, David and Abraham, Judith A. and Haaparanta, Tapio and Palisi, Thomas M. and Kirschner, Marc W.}, year={1988}, month=nov, pages={1053–1056} }

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}